Construct: ORF ccsbBroad304_15004

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008549.1_s304c1, BRDN0000462425
Derived from:
ccsbBroadEn_15004
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
MYO16 (23026)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15004
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23026 MYO16 myosin XVI NM_015011.3 99.8% 47% (many diffs)
2 human 23026 MYO16 myosin XVI XM_011521062.1 99.8% 47% (many diffs)
3 human 23026 MYO16 myosin XVI NM_001198950.3 98.6% 46.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2808
ORF length:
2739
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggaaatcgac cagtgcttgc tagagtccct tccccttggc caacggcagc
121gtctagtgaa gcgcatgcgc tgtgagcaaa tcaaagccta ctatgagcgc gagaaggctt
181ttcagaagca ggaagggttc ctgaaaaggc tgaagcatgc gaagaatccg aaagttcact
241tcaacctcac ggacatgcta caggacgcga ttatccacca caatgacaaa gaagtgcttc
301ggctcctgaa ggagggggca gacccccaca ccctcgtctc ctcgggaggg tccctgctcc
361atctgtgtgc tcggtatgat aatgccttca ttgcagaaat tctgattgac agaggagtca
421acgtcaacca ccaggatgaa gacttctgga cgcccatgca cattgcctgt gcctgcgata
481accctgatat tgtcctgctt cttgtattag ctggagccaa tgtccttctc caggatgtga
541atggaaatat cccattagat tatgctgtag aagggacaga atccagctct atcctgttga
601cctatctgga tgaaaatgga gtggatttga cctcactgcg ccagatgaag cttcagagac
661caatgagtat gttaacagat gtcaaacact tcttatcatc tggaggaaat gtcaatgaga
721aaaacgatga aggagtaacc ctgttacaca tggcgtgtgc gagtggctac aaggaggtgg
781tgtctcttat cctggaacat ggtggagacc tcaacatagt agatgatcag tactggactc
841ccctccactt ggcagccaaa tatggccaga caaatctggt gaaacttctc ctgatgcatc
901aggcaaaccc acacctcgtg aactgtaatg aggagaaggc gtcagatatt gctgcctctg
961agtttattga ggaaatgctg ctgaaagccg aaattgcctg ggaagaaaaa atgaaagagc
1021ctttatctgc ttctacctta gctcaagaag agccctatga agagatcatt cacgatcttc
1081ccgtactgtc gagtaagctc agtcccctgg tgttaccaat tgccaagcaa gacagtttgt
1141tggaaaaaga cattatgttc aaagatgcaa caaaaggtct gtgtaagcag cagtctcagg
1201acagcatccc tgaaaacccc atgatgagcg gttccaccaa acccgagcag gtcaagctaa
1261tgcctcctgc cccaaacgat gacctggcaa cgctcagcga gctcaatgat ggcagcctgc
1321tctatgagat tcagaagcgc tttgggaaca atcagatcta tacattcatt ggagacattc
1381ttttgcttgt taacccatac aaggagcttc caatttattc ttccatggtg tcccagctgt
1441atttcagctc ctcagggaag ctgtgttcct cgctgcctcc tcacctcttc tcctgtgtgg
1501agagagcctt tcaccagctc ttccgggaac agcggcctca gtgtttcatc ctcagtggag
1561aaaggggatc aggaaagtct gaagccagca aacaaatcat aagacacctc acctgcaggg
1621ctggcgccag cagggccaca ctggattcca gattcaaaca tgtcgtgtgc atcttagaag
1681cctttggaca tgccaagacc acacttaatg atttgtccag ttgcttcatc aagtattttg
1741aacttcagtt ctgtgagagg aaacaacagc taaccggagc cagaatttat acatatttgc
1801tagagaaatc cagacttgtt tcacaacctc ttggccagag caattttctc attttctact
1861tgttgatgga tgggttatct gctgaagaaa aatatggact tcatcttaat aatttatgtg
1921cacaccggta tttgaaccag accatacagg atgatgcatc cacaggggag cgttctctga
1981acagggagaa attggctgtt ttgaaacgag ccctgaatgt agttggcttc agcagcttgg
2041aggtggagaa tctgttcgta attctagcag caatattgca ccttggagac attcggttta
2101ctgccctgaa tgaggggaac tccgccttcg tttctgacct ccagctcctg gaacaagtgg
2161ctggaatgtt acaagtatca acagatgaat tggcatctgc cttaacaact gatattcaat
2221attttaaagg ggatatgata atacgacgac ataccataca gattgctgag tttttccgag
2281acctcttggc caagtccctg tacagtcgtt tgtttagctt tttggtgaat accatgaatt
2341cttgcctcca cagtcaagat gaacagaaaa gcatgcagac attggatatt ggaatattgg
2401acatttttgg ttttgaagag tttcaaaaga atgaatttga acaactttgt gtcaacatga
2461ccaatgagaa gatgcaccac tatatcaatg aagtgctttt tctccacgag caagtggaat
2521gtgtacaaga gggagttacc atggaaacag catattctgc tggtaaccag aatggagttt
2581tggacttttt tttccagaag ccatctggat ttctcacctt attggatgaa gaaagtcaaa
2641tgatttggtc agtggaatca aattttccaa aaaaactaca aaagtctcct agaatcctca
2701aacacaaatg cggtgtactc ccccatgaag gatgggaatg ggaatgttgc cctcaaagac
2761cacggtacag ccttcaccat catgcactac gcaggaaggg taatgtatga tgttgttggg
2821gcgattgaaa aaataaagac tccctttcac agaatcttct atttgtaatg aaaactagtg
2881aaaatgtcgt gatcaatcat ttgttccagt cgaaattgtc acaaacagga tccctcgtat
2941ctgcctatcc ttcctttaaa ttccgaggac ataagtctgc cctgctcagt aagaaaatga
3001cagcttcttc aattattgga gaaaacaaga attatntaga acttagtaag ttattaaaaa
3061agaaaggaac ttctacattt cttcaaagat tggaacgagg agatccagtc accatagcat
3121cacaactcag gaaatcacta atggatatta ttggaaaact tcagaagtgc actccacact
3181tcattcattg catcaggccc aataactcaa agctgccaga tacttttgat aatttttacg
3241tgtctgctca gctacaatat attggggtcc tggagatggt gaagatcttc cgatatggat
3301accctgttcg cctttccttc tcggatttcc tgtcaaggta taagccactg gctgatacat
3361tcctgcgtga gaagaaggaa cagtcagctg ccgagcgatg tcgacttgtt ctccagcagt
3421gtaaattaca aggctggcag atgggagtcc gaaaagtgtt tctaaaatac tggcatgctg
3481accaactcaa tgatttgtgc ctacagttgc agagaaaaat tataacctgc caaaaagtta
3541tcagaggatt tttagcacgc cagcacctgc ttcagagaat aagcatcaga caacaagagg
3601tgacttctat caatagcttt ctgcagaaca cagaggacat ggggctgaaa acctacgatg
3661ccctggtcat tcagaatgct tcagacattg cccgggaaaa tgaccggctc cgtagtgaaa
3721tgaacgctcc ctaccataaa gagaagttag aggtcaggaa catgcaagag gaaggaagca
3781aaagaaccga tgacaagagt ggacccaggc atttccaccc cagctccatg tcagtctgcg
3841cggccgtgga tggcctgggc cagtgcctcg ttggcccgtc catctggtct ccttcgctgc
3901actcggtgtt cagcatggat gacagcagca gcctcccgtc tccacggaaa cagcccccgc
3961ccaagccaaa gagggacccc aacacccggc tgagtgcttc ctatgaggct gtgagcgcct
4021gcctctccgc ggccagggaa gcggccaacg aagctctggc ccggcccaga ccgcacagcg
4081acgactacag caccatgaag aagattcctc ctcgaaagcc caagcgcagc cccaacacca
4141agctcagcgg ctcctacgag gagatatcgg ggtcccggcc cggggacgcg aggcccgcgg
4201gcgccccggg ggcagcagcg cgcgttctga cccccgggac tccgcagtgc gcgctgcccc
4261cggcggcgcc tccgggtgac gaggacgaca gcgagcctgt gtacatcgag atgctggggc
4321acgcggccag gcccgatagc ccggaccccg gggagtccgt gtacgaggag atgaagtgtt
4381gcctgcccga cgacggcggc ccgggcgcgg gctccttcct gctccacggc gcatcgccgc
4441ccctgctcca ccgcgcgccg gaggacgagg cggcggggcc cccaggggac gcgtgcgaca
4501tcccgccgcc cttccccaac ctgctgccgc accggccgcc cctgctggtg ttccccccga
4561cccccgtcac ctgcctcccc cgcctccgac gagtcgcccc tgaccacccc tggaggtgaa
4621gaagctgcca gtcctggaga ccaacctcaa gtaccccgtg cagccggagg ggtcgagccc
4681gctgtccccg cagtactcca agagccagaa gggcgacggc gacaggcccg cgtcccccgg
4741cctggcgctg ttcaacgggt ccggccgagc ctccccgccg tccacgccgc cccccgcccc
4801cgcccccgcc cgggccgccc cccgcgccct acaggccctg cgcgcacttg gccttcccgc
4861cggagcccgc cccggtgaac gcggggaaag cggggccgag cgcagaggcg cccaaggtcc
4921acccaaagcc aaactctgcc cccgtggccg ggccctgcag ctccttcccc aagatcccat
4981attcccccgt gaaggccacc agggcggacg ccaggaaggc cggctccagt gcctcgcccc
5041ccgcgcccta cagccctccc agctccaggc ctctcagcag ccccctggac gagctcgcca
5101gcctcttcaa ctcggggagg agtgtgcttc ggaaatccgc ggcgggaaga aaaatcaggg
5161aagccgaagg ttttgaaact aacatgaaca taagtagccg agatgaccct agtacgtcag
5221aaattacttc cgagactcaa gacagaaatg caaataacca tggaattcag ttatctaatt
5281cactatctag tgctataact gctgaaaatg gaaattccat ctcaaatggt ttacctgaag
5341aagatggata ctcacggttg tctataagtg gcacagggac ttcgacattt caaagacaca
5401gggacagtca caccactcag gtaatccatc agctgaggct ctcagagaat gaaagtgtgg
5461ccctgcagga actcttggac tggaggagaa agctctgtga ggaaggacaa gactggcagc
5521agatcctgca ccacgctgag cccagggtgc ctcccccacc accttgcaag aagcccagcc
5581ttctgaagaa gccggaaggg gcctcctgca acaggctgcc gtctgagctc tgggacacca
5641ccattttgcc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg
5701gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca
5761acctctggat ta

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