Construct: ORF ccsbBroad304_15004
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008549.1_s304c1, BRDN0000462425
- Derived from:
- ccsbBroadEn_15004
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MYO16 (23026)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15004
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23026 | MYO16 | myosin XVI | NM_015011.3 | 99.8% | 47% | (many diffs) |
| 2 | human | 23026 | MYO16 | myosin XVI | XM_011521062.1 | 99.8% | 47% | (many diffs) |
| 3 | human | 23026 | MYO16 | myosin XVI | NM_001198950.3 | 98.6% | 46.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2808
- ORF length:
- 2739
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaaatcgac cagtgcttgc tagagtccct tccccttggc caacggcagc 121 gtctagtgaa gcgcatgcgc tgtgagcaaa tcaaagccta ctatgagcgc gagaaggctt 181 ttcagaagca ggaagggttc ctgaaaaggc tgaagcatgc gaagaatccg aaagttcact 241 tcaacctcac ggacatgcta caggacgcga ttatccacca caatgacaaa gaagtgcttc 301 ggctcctgaa ggagggggca gacccccaca ccctcgtctc ctcgggaggg tccctgctcc 361 atctgtgtgc tcggtatgat aatgccttca ttgcagaaat tctgattgac agaggagtca 421 acgtcaacca ccaggatgaa gacttctgga cgcccatgca cattgcctgt gcctgcgata 481 accctgatat tgtcctgctt cttgtattag ctggagccaa tgtccttctc caggatgtga 541 atggaaatat cccattagat tatgctgtag aagggacaga atccagctct atcctgttga 601 cctatctgga tgaaaatgga gtggatttga cctcactgcg ccagatgaag cttcagagac 661 caatgagtat gttaacagat gtcaaacact tcttatcatc tggaggaaat gtcaatgaga 721 aaaacgatga aggagtaacc ctgttacaca tggcgtgtgc gagtggctac aaggaggtgg 781 tgtctcttat cctggaacat ggtggagacc tcaacatagt agatgatcag tactggactc 841 ccctccactt ggcagccaaa tatggccaga caaatctggt gaaacttctc ctgatgcatc 901 aggcaaaccc acacctcgtg aactgtaatg aggagaaggc gtcagatatt gctgcctctg 961 agtttattga ggaaatgctg ctgaaagccg aaattgcctg ggaagaaaaa atgaaagagc 1021 ctttatctgc ttctacctta gctcaagaag agccctatga agagatcatt cacgatcttc 1081 ccgtactgtc gagtaagctc agtcccctgg tgttaccaat tgccaagcaa gacagtttgt 1141 tggaaaaaga cattatgttc aaagatgcaa caaaaggtct gtgtaagcag cagtctcagg 1201 acagcatccc tgaaaacccc atgatgagcg gttccaccaa acccgagcag gtcaagctaa 1261 tgcctcctgc cccaaacgat gacctggcaa cgctcagcga gctcaatgat ggcagcctgc 1321 tctatgagat tcagaagcgc tttgggaaca atcagatcta tacattcatt ggagacattc 1381 ttttgcttgt taacccatac aaggagcttc caatttattc ttccatggtg tcccagctgt 1441 atttcagctc ctcagggaag ctgtgttcct cgctgcctcc tcacctcttc tcctgtgtgg 1501 agagagcctt tcaccagctc ttccgggaac agcggcctca gtgtttcatc ctcagtggag 1561 aaaggggatc aggaaagtct gaagccagca aacaaatcat aagacacctc acctgcaggg 1621 ctggcgccag cagggccaca ctggattcca gattcaaaca tgtcgtgtgc atcttagaag 1681 cctttggaca tgccaagacc acacttaatg atttgtccag ttgcttcatc aagtattttg 1741 aacttcagtt ctgtgagagg aaacaacagc taaccggagc cagaatttat acatatttgc 1801 tagagaaatc cagacttgtt tcacaacctc ttggccagag caattttctc attttctact 1861 tgttgatgga tgggttatct gctgaagaaa aatatggact tcatcttaat aatttatgtg 1921 cacaccggta tttgaaccag accatacagg atgatgcatc cacaggggag cgttctctga 1981 acagggagaa attggctgtt ttgaaacgag ccctgaatgt agttggcttc agcagcttgg 2041 aggtggagaa tctgttcgta attctagcag caatattgca ccttggagac attcggttta 2101 ctgccctgaa tgaggggaac tccgccttcg tttctgacct ccagctcctg gaacaagtgg 2161 ctggaatgtt acaagtatca acagatgaat tggcatctgc cttaacaact gatattcaat 2221 attttaaagg ggatatgata atacgacgac ataccataca gattgctgag tttttccgag 2281 acctcttggc caagtccctg tacagtcgtt tgtttagctt tttggtgaat accatgaatt 2341 cttgcctcca cagtcaagat gaacagaaaa gcatgcagac attggatatt ggaatattgg 2401 acatttttgg ttttgaagag tttcaaaaga atgaatttga acaactttgt gtcaacatga 2461 ccaatgagaa gatgcaccac tatatcaatg aagtgctttt tctccacgag caagtggaat 2521 gtgtacaaga gggagttacc atggaaacag catattctgc tggtaaccag aatggagttt 2581 tggacttttt tttccagaag ccatctggat ttctcacctt attggatgaa gaaagtcaaa 2641 tgatttggtc agtggaatca aattttccaa aaaaactaca aaagtctcct agaatcctca 2701 aacacaaatg cggtgtactc ccccatgaag gatgggaatg ggaatgttgc cctcaaagac 2761 cacggtacag ccttcaccat catgcactac gcaggaaggg taatgtatga tgttgttggg 2821 gcgattgaaa aaataaagac tccctttcac agaatcttct atttgtaatg aaaactagtg 2881 aaaatgtcgt gatcaatcat ttgttccagt cgaaattgtc acaaacagga tccctcgtat 2941 ctgcctatcc ttcctttaaa ttccgaggac ataagtctgc cctgctcagt aagaaaatga 3001 cagcttcttc aattattgga gaaaacaaga attatntaga acttagtaag ttattaaaaa 3061 agaaaggaac ttctacattt cttcaaagat tggaacgagg agatccagtc accatagcat 3121 cacaactcag gaaatcacta atggatatta ttggaaaact tcagaagtgc actccacact 3181 tcattcattg catcaggccc aataactcaa agctgccaga tacttttgat aatttttacg 3241 tgtctgctca gctacaatat attggggtcc tggagatggt gaagatcttc cgatatggat 3301 accctgttcg cctttccttc tcggatttcc tgtcaaggta taagccactg gctgatacat 3361 tcctgcgtga gaagaaggaa cagtcagctg ccgagcgatg tcgacttgtt ctccagcagt 3421 gtaaattaca aggctggcag atgggagtcc gaaaagtgtt tctaaaatac tggcatgctg 3481 accaactcaa tgatttgtgc ctacagttgc agagaaaaat tataacctgc caaaaagtta 3541 tcagaggatt tttagcacgc cagcacctgc ttcagagaat aagcatcaga caacaagagg 3601 tgacttctat caatagcttt ctgcagaaca cagaggacat ggggctgaaa acctacgatg 3661 ccctggtcat tcagaatgct tcagacattg cccgggaaaa tgaccggctc cgtagtgaaa 3721 tgaacgctcc ctaccataaa gagaagttag aggtcaggaa catgcaagag gaaggaagca 3781 aaagaaccga tgacaagagt ggacccaggc atttccaccc cagctccatg tcagtctgcg 3841 cggccgtgga tggcctgggc cagtgcctcg ttggcccgtc catctggtct ccttcgctgc 3901 actcggtgtt cagcatggat gacagcagca gcctcccgtc tccacggaaa cagcccccgc 3961 ccaagccaaa gagggacccc aacacccggc tgagtgcttc ctatgaggct gtgagcgcct 4021 gcctctccgc ggccagggaa gcggccaacg aagctctggc ccggcccaga ccgcacagcg 4081 acgactacag caccatgaag aagattcctc ctcgaaagcc caagcgcagc cccaacacca 4141 agctcagcgg ctcctacgag gagatatcgg ggtcccggcc cggggacgcg aggcccgcgg 4201 gcgccccggg ggcagcagcg cgcgttctga cccccgggac tccgcagtgc gcgctgcccc 4261 cggcggcgcc tccgggtgac gaggacgaca gcgagcctgt gtacatcgag atgctggggc 4321 acgcggccag gcccgatagc ccggaccccg gggagtccgt gtacgaggag atgaagtgtt 4381 gcctgcccga cgacggcggc ccgggcgcgg gctccttcct gctccacggc gcatcgccgc 4441 ccctgctcca ccgcgcgccg gaggacgagg cggcggggcc cccaggggac gcgtgcgaca 4501 tcccgccgcc cttccccaac ctgctgccgc accggccgcc cctgctggtg ttccccccga 4561 cccccgtcac ctgcctcccc cgcctccgac gagtcgcccc tgaccacccc tggaggtgaa 4621 gaagctgcca gtcctggaga ccaacctcaa gtaccccgtg cagccggagg ggtcgagccc 4681 gctgtccccg cagtactcca agagccagaa gggcgacggc gacaggcccg cgtcccccgg 4741 cctggcgctg ttcaacgggt ccggccgagc ctccccgccg tccacgccgc cccccgcccc 4801 cgcccccgcc cgggccgccc cccgcgccct acaggccctg cgcgcacttg gccttcccgc 4861 cggagcccgc cccggtgaac gcggggaaag cggggccgag cgcagaggcg cccaaggtcc 4921 acccaaagcc aaactctgcc cccgtggccg ggccctgcag ctccttcccc aagatcccat 4981 attcccccgt gaaggccacc agggcggacg ccaggaaggc cggctccagt gcctcgcccc 5041 ccgcgcccta cagccctccc agctccaggc ctctcagcag ccccctggac gagctcgcca 5101 gcctcttcaa ctcggggagg agtgtgcttc ggaaatccgc ggcgggaaga aaaatcaggg 5161 aagccgaagg ttttgaaact aacatgaaca taagtagccg agatgaccct agtacgtcag 5221 aaattacttc cgagactcaa gacagaaatg caaataacca tggaattcag ttatctaatt 5281 cactatctag tgctataact gctgaaaatg gaaattccat ctcaaatggt ttacctgaag 5341 aagatggata ctcacggttg tctataagtg gcacagggac ttcgacattt caaagacaca 5401 gggacagtca caccactcag gtaatccatc agctgaggct ctcagagaat gaaagtgtgg 5461 ccctgcagga actcttggac tggaggagaa agctctgtga ggaaggacaa gactggcagc 5521 agatcctgca ccacgctgag cccagggtgc ctcccccacc accttgcaag aagcccagcc 5581 ttctgaagaa gccggaaggg gcctcctgca acaggctgcc gtctgagctc tgggacacca 5641 ccattttgcc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 5701 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 5761 acctctggat ta