Transcript: Human XM_011521062.1

PREDICTED: Homo sapiens myosin XVI (MYO16), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MYO16 (23026)
Length:
6980
CDS:
235..5811

Additional Resources:

NCBI RefSeq record:
XM_011521062.1
NBCI Gene record:
MYO16 (23026)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011521062.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000445134 ATGAACGCTCCCTACCATAAA pLKO_005 3886 CDS 100% 13.200 18.480 N MYO16 n/a
2 TRCN0000150310 CGCACCTAAATCTTGAAACTA pLKO.1 6421 3UTR 100% 5.625 7.875 N MYO16 n/a
3 TRCN0000444895 ATTCACTATCTAGTGCTATAA pLKO_005 5441 CDS 100% 13.200 10.560 N MYO16 n/a
4 TRCN0000151510 CCAGTTGCTTCATCAAGTATT pLKO.1 1883 CDS 100% 13.200 9.240 N MYO16 n/a
5 TRCN0000155527 GCCTGCGATAACCCTGATATT pLKO.1 637 CDS 100% 13.200 9.240 N MYO16 n/a
6 TRCN0000150309 CGGTATGATAATGCCTTCATT pLKO.1 538 CDS 100% 5.625 3.938 N MYO16 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011521062.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15004 pDONR223 97.7% 99.8% 47% None (many diffs) n/a
2 ccsbBroad304_15004 pLX_304 0% 99.8% 47% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000479281 GTACTGCACTCCCCCCACAGACTG pLX_317 7.9% 99.8% 47% V5 (not translated due to prior stop codon) (many diffs) n/a
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