Construct: ORF ccsbBroad304_15023
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006074.2_s304c1, BRDN0000459498
- Derived from:
- ccsbBroadEn_15023
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRKD3 (23683)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15023
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23683 | PRKD3 | protein kinase D3 | NM_005813.5 | 67.9% | 65.8% | (many diffs) |
| 2 | human | 23683 | PRKD3 | protein kinase D3 | XM_005264237.4 | 67.9% | 65.8% | (many diffs) |
| 3 | human | 23683 | PRKD3 | protein kinase D3 | XM_024452776.1 | 67.9% | 65.8% | (many diffs) |
| 4 | human | 23683 | PRKD3 | protein kinase D3 | XM_005264238.3 | 56.2% | 54.3% | (many diffs) |
| 5 | human | 23683 | PRKD3 | protein kinase D3 | XM_017003741.2 | 48.5% | 46.7% | (many diffs) |
| 6 | human | 23683 | PRKD3 | protein kinase D3 | XM_017003742.2 | 48.5% | 46.7% | (many diffs) |
| 7 | human | 23683 | PRKD3 | protein kinase D3 | XM_017003743.2 | 38.9% | 37.2% | (many diffs) |
| 8 | human | 23683 | PRKD3 | protein kinase D3 | XM_017003744.2 | 38.9% | 37.2% | (many diffs) |
| 9 | human | 23683 | PRKD3 | protein kinase D3 | XM_024452777.1 | 38.9% | 37.2% | (many diffs) |
| 10 | mouse | 75292 | Prkd3 | protein kinase D3 | NM_001171004.1 | 59.9% | 62.2% | (many diffs) |
| 11 | mouse | 75292 | Prkd3 | protein kinase D3 | NM_029239.3 | 59.7% | 62% | (many diffs) |
| 12 | mouse | 75292 | Prkd3 | protein kinase D3 | NM_001171005.1 | 50.4% | 52.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1899
- ORF length:
- 1833
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc tgcaaataat tcccctccat cagcccagaa gtctgtatta cccacagcta 121 ttcctgctgt gcttccagct gcttctccgt gttcaagtcc taagacggga ctctctgccc 181 gactctctaa tggaagcttc agtgcaccat cactcaccaa ctccagaggc tcagtgcata 241 cagtttcatt tctactgcaa attggcctca cacgggagag tgttaccatt gaagcccagg 301 aactgtcttt atctgctgtc aaggatcttg tgtgctccat agtttatcaa aagtttccag 361 agtgtggatt ctttggcatg tatgacaaaa ttcttctctt tcgccatgac atgaactcag 421 aaaacatttt gcagctgatt acctcaacag atgaaataca tgaaggagac ctagtggaag 481 tggttctttc agctttagcc acagtagaag acttccagat tcgtccacat actctctatg 541 tacattctta caaagctcct actttctgtg attactgtgg tgagatgctc tggggattgg 601 tacgtcaagg actgaaatgt gaaggctgtg gattaaatta ccataaacga tgtgccttca 661 agattccaaa taactgtagt ggagtaagaa agagacgtct gtcaaatgta tctttaccag 721 gacccggcct ctcagttcca agacccctac agcctgaata tgtagccctt cccagtgaag 781 agtcacatgt ccaccaggaa ccaagtaaga gaattccttc ttggagtggt cgcccaatct 841 ggatggaaaa gatggtaatg tgcagagtga aagttccaca cacatttgct gttcactctt 901 acacccgtcc cacgatatgt cagtactgca agcggttact gaaaggcctc tttcgccaag 961 gaatgcagtg taaagattgc aaattcaact gccataaacg ctgtgcatca aaagtaccaa 1021 gagactgcct tggagaggtt actttcaatg gagaaccttc cagtctggga acagatacag 1081 atataccaat ggatattgac aataatgaca taaatagtga tagtagtcgg ggtttggatg 1141 acacagaaga gccatcaccc ccagaagata agatgttctt cttggatcca tctgatctcg 1201 atgtggaaag agatgaagaa gccgttaaaa caatcagtcc atcaacaagc aataatattc 1261 cgctaatgag ggttgtacaa tccatcaagc acacaaagag gaagagcagc acaatggtga 1321 aggaagggtg gatggtccat tacaccagca gggataacct gagaaagagg cattattgga 1381 gacttgacag caaatgtcta acattatttc agaatgaatc tggatcaaag tattataagg 1441 aaattccact ttcagaaatt ctccgcatat cttcaccacg agatttcaca aacatttcac 1501 aaggcagcaa tccacactgt tttgaaatca ttactgatac tatggtatac ttcgttggtg 1561 agaacaatgg ggacagctct cataatcctg ttcttgctgc cactggagtt ggacttgatg 1621 tagcacagag ctgggaaaaa gcaattcgcc aagccctcat gcctgttact cctcaagcaa 1681 gtgtttgcac ttctccaggg cgagggaaag atcacaaaga tttgtctaca agtatctctg 1741 tatctaattg tcagattcag gagaatgtgg atatcagtac tgtttaccag atctttgcag 1801 atgaggtgct tggttcaggc cagtttggca tcgtttatgg aggtaagcaa ctacagccgt 1861 ttgcatattg tacacattat tttaaaaact ggaaaatgta cccaactttc ttgtacaaag 1921 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 1981 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta