Construct: ORF ccsbBroad304_15044
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018976.1_s304c1, BRDN0000459459
- Derived from:
- ccsbBroadEn_15044
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- RPS6KA3 (6197)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15044
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27330 | RPS6KA6 | ribosomal protein S6 kinase A6 | NM_014496.5 | 91% | 28% | (many diffs) |
| 2 | human | 27330 | RPS6KA6 | ribosomal protein S6 kinase A6 | NM_001330512.1 | 87.9% | 24.2% | (many diffs) |
| 3 | human | 27330 | RPS6KA6 | ribosomal protein S6 kinase A6 | XM_011530917.2 | 87.9% | 24.2% | (many diffs) |
| 4 | human | 27330 | RPS6KA6 | ribosomal protein S6 kinase A6 | XM_017029423.2 | 86.2% | 23.6% | (many diffs) |
| 5 | human | 27330 | RPS6KA6 | ribosomal protein S6 kinase A6 | XM_017029424.1 | 86.2% | 23.6% | (many diffs) |
| 6 | human | 27330 | RPS6KA6 | ribosomal protein S6 kinase A6 | XM_017029425.1 | 86.2% | 23.6% | (many diffs) |
| 7 | human | 27330 | RPS6KA6 | ribosomal protein S6 kinase A6 | XM_011530919.2 | 77.6% | 14.2% | (many diffs) |
| 8 | human | 27330 | RPS6KA6 | ribosomal protein S6 kinase A6 | XM_011530920.2 | 75.2% | 11.8% | (many diffs) |
| 9 | human | 27330 | RPS6KA6 | ribosomal protein S6 kinase A6 | XM_017029426.1 | 59.8% | 11.8% | (many diffs) |
| 10 | mouse | 67071 | Rps6ka6 | ribosomal protein S6 kinase... | XM_011247707.2 | 79.4% | 23% | (many diffs) |
| 11 | mouse | 67071 | Rps6ka6 | ribosomal protein S6 kinase... | XM_006528404.2 | 77% | 24.1% | (many diffs) |
| 12 | mouse | 67071 | Rps6ka6 | ribosomal protein S6 kinase... | XM_006528403.2 | 71.8% | 22.4% | (many diffs) |
| 13 | mouse | 67071 | Rps6ka6 | ribosomal protein S6 kinase... | NM_025949.3 | 70.1% | 21.9% | (many diffs) |
| 14 | mouse | 67071 | Rps6ka6 | ribosomal protein S6 kinase... | XM_006528400.3 | 70.1% | 21.9% | (many diffs) |
| 15 | mouse | 67071 | Rps6ka6 | ribosomal protein S6 kinase... | XM_006528401.3 | 70.1% | 21.9% | (many diffs) |
| 16 | mouse | 67071 | Rps6ka6 | ribosomal protein S6 kinase... | XM_006528402.2 | 69.7% | 22% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 705
- ORF length:
- 639
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgct accattcgct cctcaggacg agccctggga ccgagaaatg gaagtgttca 121 gcggcggcgg cgcgagcagc ggcgaggtaa atggtcttaa aatggttgat gagccaatgg 181 aagagggaga agcagattct tgtcatgatg aaggagttgt taaagaaatc cctattactc 241 atcatgttaa ggaaggctat gagaaagcag atcctgcaca gtttgagttg ctcaaggttc 301 ttggtcaggg gtcatttgga aaggtttttc ttgttagaaa gaagaccggt cctgatgctg 361 ggcagctcta tgcaatgaag gtgttaaaaa aagcctcttt aaaagttcga gacagagttc 421 ggacaaagat ggagagggat atactggtgg aagtaaatca tccatttatt gtcaaattgc 481 actatgcctt tcagactgaa gggaaactgt acttaatact ggattttctc aggggaggag 541 atgttttcac aagattatcc aaagaggttc tgtttacaga ggaagatgtg aaattctacc 601 tcgcagaact ggcccttgct ttggatcatc tgcaccaatt aggaattgtt tatagagacc 661 tgaagccaga aaacattttg cttgatgaaa tagacatatc aaattaacag attttggact 721 cagcaaggag tcagtagatc aagaaaagaa gcttactcat tttgtggtac agtagagtat 781 atggctcctg aagtagtaaa taggagagca tcccagagtg ctgatggtgt tcatatggtg 841 ttcttatgtt tgaatgctta ctggtactct gccatttcaa ggtaaagaca gaaatgagac 901 aatgactatg attcttaaag ccaaacttgg aatgccacag tttttgagtc ctgaagcgca 961 gagtctttta cgaatgcttt tcaagcgaaa tcctgcaaac agattaggtg caggaccaga 1021 tggagttgaa gaaattaaaa gacattcatt tttctcaacg atagactgga ataaactgta 1081 tagaagagaa attcatccgc catttaaacc tgcaacgggc aggcctgaag atacattcta 1141 ttttgatcct gagtttactg caaaaactcc caaagattca cctggcattc cacctagtgc 1201 taatgcacat cagctttttc gggggtttag ttttgttgct attacctcag atgatgaaag 1261 ccaagctatg cagacagttg gtgtacattc aattgttcag cagttacaca ggaacagtat 1321 tcagtttact gatggatatg aagtaaaaga agatattgga gttggctcct actctgtttg 1381 caagagatgt atacataaag ctacaaacat ggagtttgca gtgaagatta ttgataaaag 1441 caagagagac ccaacagaag aaattgaaat tcttctgcgc tatggacaac atccccaacc 1501 atttttatac tttgaggatt ctttgatgat ggtagatatg tttacctgta cnggattaat 1561 ggaaaggagg agagttactt gaccgtattc tcaacaaaaa atgttttctc gaacggaggg 1621 ctagtgatat actatatgta ataagtaaga cagtgnacta tcttcattgt caaggagttg 1681 ttcatcgtga tcttaaacnt agtaatatnt tatacatgga tgaatcagcc agtgcagatt 1741 caatcaggat atgtgatttt gggtttgcaa aacaacttcg aggagaaaat ggacttctct 1801 taactccatg ctacactgca aactttgttg cacctgaggt tcttatgcaa cagggatatg 1861 atgctgcttg tgatatctgg agtttaggag tcctttttta cacaatgttg gctggctaca 1921 ctccatttgc taatggcccc aatgatactc ctgaagagat actgctgcgt ataggcaatg 1981 gaaaattctc tttgagtggt ggaaactggg acaatatttc agacggagca aaggatttgc 2041 tttcccatat gcttcatatg gacccacatc agcggtatac tgctgaacaa atattaaagc 2101 actcatggat aactcacaga gaccagttgc caaatgatca gccaaagaga aatgatgtgt 2161 cacatgttgt taagggagca atggttgcaa catactctgc cctgactcac aagacctttc 2221 aaccagtcct agagcctgta gctgcttcaa gcttagccca gcgacggagc atgaaaaagc 2281 gaacatcaac tggcctgttg ccaactttct tgtacaaagt ggttggtaag cctatcccta 2341 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 2401 agtcgacaat caacctctgg atta