Construct: ORF ccsbBroad304_15051

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017117.2_s304c1, BRDN0000459643
Derived from:
ccsbBroadEn_15051
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EEF2K (29904)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15051
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 29904 EEF2K eukaryotic elongation facto... NM_013302.4 99.9% 99.8% 68A>G;489C>A
2 human 29904 EEF2K eukaryotic elongation facto... XM_017023193.2 99.8% 99.7% 68A>G;489C>A;1082A>G
3 human 29904 EEF2K eukaryotic elongation facto... XM_017023194.2 96.9% 96.8% (many diffs)
4 human 29904 EEF2K eukaryotic elongation facto... XR_950786.3 78.3% (many diffs)
5 human 29904 EEF2K eukaryotic elongation facto... XR_001751897.2 72.8% (many diffs)
6 human 29904 EEF2K eukaryotic elongation facto... XR_950787.3 51.6% (many diffs)
7 mouse 13631 Eef2k eukaryotic elongation facto... NM_001267710.1 85.7% 89.9% (many diffs)
8 mouse 13631 Eef2k eukaryotic elongation facto... NM_001267711.1 85.7% 89.9% (many diffs)
9 mouse 13631 Eef2k eukaryotic elongation facto... NM_007908.4 85.7% 89.9% (many diffs)
10 mouse 13631 Eef2k eukaryotic elongation facto... XM_006507342.3 85.7% 89.9% (many diffs)
11 mouse 13631 Eef2k eukaryotic elongation facto... XM_006507343.3 85.7% 89.9% (many diffs)
12 mouse 13631 Eef2k eukaryotic elongation facto... XM_006507344.3 80.4% 84.5% (many diffs)
13 mouse 13631 Eef2k eukaryotic elongation facto... XM_006507345.3 72.9% 76.4% (many diffs)
14 mouse 13631 Eef2k eukaryotic elongation facto... XM_006507346.3 69.6% 73.5% (many diffs)
15 mouse 13631 Eef2k eukaryotic elongation facto... XR_378214.2 45.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2241
ORF length:
2175
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc agacgaagat ctcatcttcc gcctggaagg cgttgatggc ggccagtccc
121cccgagctgg ccgtgatggt gattctgatg gggacagcga cgatgaggaa ggttacttca
181tctgccccat cacggatgac ccaagctcga accagaatgt caattccaag gttaataagt
241actacagcaa cctaacaaaa agtgagcggt atagctccag cgggtccccg gcaaactcct
301tccacttcaa ggaagcctgg aagcacgcaa tccagaaggc caagcacatg cccgacccct
361gggctgagtt ccacctggaa gatattgcca ccgaacgtgc tactcgacac aggtacaacg
421ccgtcaccgg ggaatggctg gatgatgaag ttctgatcaa gatggcatct cagcccttcg
481gccgaggagc aatgagggag tgcttccgga cgaagaagct ctccaacttc ttgcatgccc
541agcagtggaa gggagcctcc aactacgtgg cgaagcgcta catcgagccc gtagaccggg
601atgtgtactt tgaggacgtg cgtctacaga tggaggccaa gctctggggg gaggagtata
661atcggcacaa gccccccaag caggtggaca tcatgcagat gtgcatcatc gagctgaagg
721acagaccggg caagcccctc ttccacctgg agcactacat cgagggcaag tacatcaagt
781acaactccaa ctctggcttt gtccgcgatg acaacatccg cctgacgccg caggccttca
841gccacttcac ttttgagcgt tccggccatc agctgatagt ggtggacatc cagggagttg
901gggatctcta cactgaccca cagatccaca cggagacggg cactgacttt ggagacggca
961acctaggtgt ccgcgggatg gcgctcttct tctactctca tgcctgcaac cggatttgcg
1021agagcatggg ccttgctccc tttgacctct cgccccggga gagggatgca gtgaatcaga
1081acaccaagct gctgcaatca gccaagacca tcttgagagg aacagaggaa aaatgtggga
1141gcccccgagt aaggaccctc tctgggagcc ggccacccct gctccgtccc ctttcagaga
1201actctggaga cgagaacatg agcgacgtga ccttcgactc tctcccttct tccccatctt
1261cggccacacc acacagccag aagctagacc acctccattg gccagtgttc agtgacctcg
1321ataacatggc atccagagac catgatcatc tagacaacca ccgggagtct gagaatagtg
1381gggacagcgg ataccccagt gagaagcggg gtgagctgga tgaccctgag ccccgagaac
1441atggccactc atacagtaat cggaagtacg agtctgacga agacagcctg ggcagctctg
1501gacgggtatg tgtagagaag tggaatctcc tcaactcctc ccgcctccac ctgccgaggg
1561cttcggccgt ggccctggaa gtgcaaaggc ttaatgctct ggacctcgaa aagaaaatcg
1621ggaagtccat tttggggaag gtccatctgg ccatggtgcg ctaccacgag ggtgggcgct
1681tctgcgagaa gggcgaggag tgggaccagg agtcggctgt cttccacctg gagcacgcag
1741ccaacctggg cgagctggag gccatcgtgg gcctgggact catgtactcg cagttgcctc
1801atcacatcct agccgatgtc tctctgaagg agacagaaga gaacaaaacc aaaggatttg
1861attacttact aaaggccgct gaagctggcg acaggcagtc catgatccta gtggcgcgag
1921cttttgactc tggccagaac ctcagcccgg acaggtgcca agactggcta gaggccctgc
1981actggtacaa cactgccctg gagatgacgg actgtgatga gggcggtgag tacgacggaa
2041tgcaggacga gccccggtac atgatgctgg ccagggaggc cgagatgctg ttcacaggag
2101gctacgggct ggagaaggac ccgcagagat caggggactt gtatacccag gcagcagagg
2161cagcgatgga agccatgaag ggccgactgg ccaaccagta ctaccaaaag gctgaagagg
2221cctgggccca gatggaggag tacccaactt tcttgtacaa agtggttggt aagcctatcc
2281ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg
2341ttaagtcgac aatcaacctc tggatta

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