Construct: ORF ccsbBroad304_15091
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018855.1_s304c1, BRDN0000459339
- Derived from:
- ccsbBroadEn_15091
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PANK4 (55229)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15091
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 55229 | PANK4 | pantothenate kinase 4 (inac... | NM_018216.4 | 72.9% | 4.4% | (many diffs) |
2 | human | 55229 | PANK4 | pantothenate kinase 4 (inac... | XR_241034.3 | 64.4% | (many diffs) | |
3 | human | 55437 | STRADB | STE20 related adaptor beta | XM_005246669.2 | 22.6% | 41.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 480
- ORF length:
- 411
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gaaaaacaag tctgaaacca gtatccatca atacttggtc gaagagccaa 121 ccctttcctg gtcgcctcca tccactagag ccagtgaagt agtatgttcc gccaacgttt 181 ctcactacga gctccaagta gaaataggaa gaggatttga caagttgact tctgtccatc 241 tagcacggca tactcctacg ggaacgctgg taactacaaa aattacaaat ctggaaaacg 301 gcaataaaga acgcctgaaa gttttacaga aagccatgat tctatcccac tttttccggc 361 atcccaatat tacaacttat tggacagttt tcactgttgg cagctggctt tgggttattt 421 ctccatttat ggcctatggt tcagcaagac aactcttgag gacctatttt cacagtaaat 481 agtgaccgat tacacacacc atcctcaaaa accttctctc ctgccttctt tagctgggta 541 cagctgtgtt tgcaacaaga tccggagaaa aggccatcag caagcagttt attgtcccat 601 gttttcttca aacagatgaa agaagaaagc caggattcgg tactttcact gttgcctcct 661 gcttataaca agccatcaat atcactgcct ccggtgttan cntggactga gccagaatgt 721 ggttttcctn ntnnanctng ntncatattg ggnntnnntn nnnaacaaga tccggagaaa 781 aggccatcag caagcagttt attgtcccat gntttcttnn anaaagttgg cattanagat 841 gaaagaagaa agccagnatn cnnatcattt gtgcacgaca gtcactatca gtcaaatnac 901 tttcactgtt gcctcctgct tataacaagc catcantatn actgcntcng ntgntncctt 961 ggactgagcn anaangtggt tttccngnng natananatt catattggga attcttgcca 1021 actttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa 1081 caggtcacta tcagtcaaaa taaaatcatt attgccatcc agcgatccta aacctggcct 1141 cgaggggcca gcacagcaat gtggacatgc tggtgcggga cgtctacggc ggcgcccacc 1201 agactctcgg gctgagcggg aacctcatcg ccagcagctt cgggaagtcg gccaccgccg 1261 accaagagtt ctccaaagaa gacatggcga agagcctgct gcacatgatc agcaacgaca 1321 ttgggcagct ggcctgcctc cacgcacggc tgcacagcct ggaccgcgtg tactttggag 1381 gcttctttat ccggggccac cccgtgacca tgcgcaccat cacctatagc atcaacttct 1441 tctccaaggg ggaagtgcag gcgctgtttc tgaggcacga aggctacctg ggagccatcg 1501 gagcgttcct gaaaggagct gagcaggaca atcctaacca gtacagctgg ggagagaact 1561 atgcaggcag ctccgggctg atgagtgcat cacccgagct cggcccggcg cagcgggcgc 1621 ggagtggcac ttttgacttg ctggaaatgg accggctgga gaggccactg gttgacctgc 1681 cgctcctcct ggacccgccc tcctacgtgc ccgacacggt ggacctcacc gatgacgctc 1741 tggcccgaaa atactggctc acctgctttg aggaggccct ggacggggta gtgaagcgcg 1801 cagtggcgag ccagccagac tctgtggatg cagccgagag ggcggagaag ttccggcaga 1861 agtactggaa caagcttcag accctgaggc agcagccctt cgcctatggg accctgaccg 1921 tgcgcagcct gctggacacc agggagcact gtctgaacga gttcaacttc ccggatccct 1981 actccaaagt gaagcagcgg gagaatggcg tggcgctgag gtgcttcccc ggggtcgtgc 2041 gctccctgga cgcgctgggc tgggaggaac ggcagctggc gctggtgaaa ggcctcctgg 2101 cggggaatgt cttcgactgg ggggccaaag ccgtgtctgc tgtccttgaa tccgacccct 2161 actttgggtt tgaagaagca aagaggaagt tacaagaaag accctggctc gtggattcct 2221 acagcgagtg gcttcagaga ttaaaggggc cccctcataa atgtgcctta attttcgcag 2281 ataacagtgg aatagacatc attttgggag tcttcccctt tgtcagggag ctactcctta 2341 gagggacaga ggtcatcctg gcgtgcaact caggccccgc cctgaacgac gtgacccaca 2401 gcgagtccct catcgtggca gagcgtattg cgggcatgga ccctgtcgtg cactctgcgc 2461 tccaggaaga gaggctgctg ctggtgcaga cgggctccag ctccccgtgc ctcgacctca 2521 gccgcctgga taaggggctg gccgcactgg tgcgggagcg tggcgcggat ctggtggtca 2581 tcgagggcat gggccgtgct gtccacacaa actaccacgc agccctgcgc tgcgagagcc 2641 tcaagctggc cgtcatcaag aacgcgtggc tggccgagcg gctgggcggc cggctcttca 2701 gcgtcatctt caagtacgag gtcccagccg agttgccaac tttcttgtac aaagtggttg 2761 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 2821 accggtggcg cgttaagtcg acaatcaacc tctggatta