Construct: ORF ccsbBroad304_15164
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008223.1_s304c1, BRDN0000461958
- Derived from:
- ccsbBroadEn_15164
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CWH43 (80157)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15164
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80157 | CWH43 | cell wall biogenesis 43 C-t... | NM_025087.3 | 98.7% | 61.3% | (many diffs) |
| 2 | human | 80157 | CWH43 | cell wall biogenesis 43 C-t... | XM_011513755.1 | 96.1% | 59.7% | (many diffs) |
| 3 | human | 80157 | CWH43 | cell wall biogenesis 43 C-t... | NM_001286791.1 | 94.9% | 57.6% | (many diffs) |
| 4 | human | 80157 | CWH43 | cell wall biogenesis 43 C-t... | XM_011513756.3 | 92.4% | 56.1% | (many diffs) |
| 5 | human | 80157 | CWH43 | cell wall biogenesis 43 C-t... | XM_011513757.2 | 92.4% | 56.1% | (many diffs) |
| 6 | human | 80157 | CWH43 | cell wall biogenesis 43 C-t... | XM_011513758.1 | 85.1% | 48.6% | (many diffs) |
| 7 | human | 80157 | CWH43 | cell wall biogenesis 43 C-t... | XM_011513759.1 | 70.1% | 33.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1386
- ORF length:
- 1317
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gacctcgctg tggagagaaa tcctcttgga gtcgctgctg ggatgtgttt 121 cttggtctct ctaccatgac ctgggaccga tgatctatta ctttcctttg caaacactag 181 aactcactgg gcttgaaggt tttagtatag catttctttc tccaatattc ctaacaatta 241 ctcctttctg gaaattggtt aacaagaagt ggatgctaac cctgctgagg ataatcacta 301 ttggcagcat agcctccttc caggctccaa atgccaaact tcgactgatg gttcttgcgc 361 ttggggtgtc ttcctcactg atagtgcaag ctgtgacttg gtggtcagga agtcatttgc 421 aaaggtacct cagaatttgg ggattcattt taggacagat tgttcttgtt gttccacgca 481 tatggtatac ttcactaaac ccaatctgga gttatcagat gtccaacaaa gtgatactga 541 cattaagtgc catagccaca cttgatcgta ttggcacaga tggtgactgc agtaaacctg 601 aagaaaagaa gactggtgag gtagccacgg ggatggcctc tagacccaac tggctgctgg 661 caggggctgc ttttggtagc cttgtgttcc tcacccactg ggtttttgga gaagtctctc 721 ttgtttccag atgggcagtg agtgggcatc cacatccagg gccagatcct aacccatttg 781 gaggtgcagt actgctgtgc ttggcaagtg gattgatgct tccatcttgt ttgtggtttc 841 gtggtactgg tttgatctgg tgggttacag gaacagcttc agctgcgggg ctcctttacc 901 tgcacacatg ggcagctgct gtgtctggct gtgtcttcgc catctttact gcatccatgt 961 ggccccaaac acttggacac cttattaact cagggacaaa ccctgggaaa accatgacca 1021 ttgccatgat attttatgca tatgagacac tagctgtagc ctggtgcaca gcctttaagt 1081 ttgtcccagg aggtgtctac gctagagaaa gatcagatgt gcttttgggg acaatgatgt 1141 taattatcgg gctgaatatg ctatttggtc ctaagaaaaa ccttgacttg cttcttcaaa 1201 caaaaaacag ttctaaagtg cttttcagaa agagtgaaaa atacatgaaa ctttttctgt 1261 ggctgcttgt tggtgtggga ttgttgggat taggactacg gcataaagcc tatgagagaa 1321 aactgggcaa agtggcacca accaaagagg tctctgctgc catctggcct ttcaggtttt 1381 ggatatgaca atgaagggtg gtctagtcta gaaagatcag ctcacctgct caatgaaaca 1441 ggtgcagatt tcataacaat tttggagagt gatgcttcta agccctatat ggggaacaat 1501 gacttaacca tgtggctagg ggaaaagttg ggtttctata cagactttgg tccaagcaca 1561 aggtatcaca cttgggggat tatggctttg tcaagatacc caattgtgaa atctgagcat 1621 caccttcttc cgtcaccaga gggcgagatc gcaccagcca tcacattgac cgttaacatt 1681 tcgggcaagc tggtggattt tgtcgtgaca cactttggga accacgaaga tgacctcgac 1741 aggaaactgc aggctattgc tgtttcaaaa ctactgaaaa gtagctctaa tcaagtgata 1801 tttctgggat atatcacttc agcacctggc tccagagatt atctacagct cactgaacat 1861 ggcaatgtga aggatatcga cagcactgat catgacagat ggtgtgaata cattatgtat 1921 cgagggctga tcaggttggg ttatgcaaga atctcccatg ctgaactgag tgattcagaa 1981 attcagatgg caaaatttag gatccctgat gaccccacta attatagaga caaccagaaa 2041 gtggtcatag accacagaga agtttctgag aaaattcatt ttaatcccag atttggatcc 2101 tacaaagaag gacacaatta tgaaaacaac canaattttc atatgaatac tcccaaatac 2161 tttttattgc caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc 2221 ggtctcgatt ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc 2281 aacctctgga tta