Construct: ORF ccsbBroad304_15164

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008223.1_s304c1, BRDN0000461958
Derived from:
ccsbBroadEn_15164
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
CWH43 (80157)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15164
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 80157 CWH43 cell wall biogenesis 43 C-t... NM_025087.3 98.7% 61.3% (many diffs)
2 human 80157 CWH43 cell wall biogenesis 43 C-t... XM_011513755.1 96.1% 59.7% (many diffs)
3 human 80157 CWH43 cell wall biogenesis 43 C-t... NM_001286791.1 94.9% 57.6% (many diffs)
4 human 80157 CWH43 cell wall biogenesis 43 C-t... XM_011513756.3 92.4% 56.1% (many diffs)
5 human 80157 CWH43 cell wall biogenesis 43 C-t... XM_011513757.2 92.4% 56.1% (many diffs)
6 human 80157 CWH43 cell wall biogenesis 43 C-t... XM_011513758.1 85.1% 48.6% (many diffs)
7 human 80157 CWH43 cell wall biogenesis 43 C-t... XM_011513759.1 70.1% 33.8% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1386
ORF length:
1317
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gacctcgctg tggagagaaa tcctcttgga gtcgctgctg ggatgtgttt
121cttggtctct ctaccatgac ctgggaccga tgatctatta ctttcctttg caaacactag
181aactcactgg gcttgaaggt tttagtatag catttctttc tccaatattc ctaacaatta
241ctcctttctg gaaattggtt aacaagaagt ggatgctaac cctgctgagg ataatcacta
301ttggcagcat agcctccttc caggctccaa atgccaaact tcgactgatg gttcttgcgc
361ttggggtgtc ttcctcactg atagtgcaag ctgtgacttg gtggtcagga agtcatttgc
421aaaggtacct cagaatttgg ggattcattt taggacagat tgttcttgtt gttccacgca
481tatggtatac ttcactaaac ccaatctgga gttatcagat gtccaacaaa gtgatactga
541cattaagtgc catagccaca cttgatcgta ttggcacaga tggtgactgc agtaaacctg
601aagaaaagaa gactggtgag gtagccacgg ggatggcctc tagacccaac tggctgctgg
661caggggctgc ttttggtagc cttgtgttcc tcacccactg ggtttttgga gaagtctctc
721ttgtttccag atgggcagtg agtgggcatc cacatccagg gccagatcct aacccatttg
781gaggtgcagt actgctgtgc ttggcaagtg gattgatgct tccatcttgt ttgtggtttc
841gtggtactgg tttgatctgg tgggttacag gaacagcttc agctgcgggg ctcctttacc
901tgcacacatg ggcagctgct gtgtctggct gtgtcttcgc catctttact gcatccatgt
961ggccccaaac acttggacac cttattaact cagggacaaa ccctgggaaa accatgacca
1021ttgccatgat attttatgca tatgagacac tagctgtagc ctggtgcaca gcctttaagt
1081ttgtcccagg aggtgtctac gctagagaaa gatcagatgt gcttttgggg acaatgatgt
1141taattatcgg gctgaatatg ctatttggtc ctaagaaaaa ccttgacttg cttcttcaaa
1201caaaaaacag ttctaaagtg cttttcagaa agagtgaaaa atacatgaaa ctttttctgt
1261ggctgcttgt tggtgtggga ttgttgggat taggactacg gcataaagcc tatgagagaa
1321aactgggcaa agtggcacca accaaagagg tctctgctgc catctggcct ttcaggtttt
1381ggatatgaca atgaagggtg gtctagtcta gaaagatcag ctcacctgct caatgaaaca
1441ggtgcagatt tcataacaat tttggagagt gatgcttcta agccctatat ggggaacaat
1501gacttaacca tgtggctagg ggaaaagttg ggtttctata cagactttgg tccaagcaca
1561aggtatcaca cttgggggat tatggctttg tcaagatacc caattgtgaa atctgagcat
1621caccttcttc cgtcaccaga gggcgagatc gcaccagcca tcacattgac cgttaacatt
1681tcgggcaagc tggtggattt tgtcgtgaca cactttggga accacgaaga tgacctcgac
1741aggaaactgc aggctattgc tgtttcaaaa ctactgaaaa gtagctctaa tcaagtgata
1801tttctgggat atatcacttc agcacctggc tccagagatt atctacagct cactgaacat
1861ggcaatgtga aggatatcga cagcactgat catgacagat ggtgtgaata cattatgtat
1921cgagggctga tcaggttggg ttatgcaaga atctcccatg ctgaactgag tgattcagaa
1981attcagatgg caaaatttag gatccctgat gaccccacta attatagaga caaccagaaa
2041gtggtcatag accacagaga agtttctgag aaaattcatt ttaatcccag atttggatcc
2101tacaaagaag gacacaatta tgaaaacaac canaattttc atatgaatac tcccaaatac
2161tttttattgc caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc
2221ggtctcgatt ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc
2281aacctctgga tta

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