Transcript: Human XM_011513759.1

PREDICTED: Homo sapiens cell wall biogenesis 43 C-terminal homolog (CWH43), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CWH43 (80157)
Length:
1925
CDS:
144..1736

Additional Resources:

NCBI RefSeq record:
XM_011513759.1
NBCI Gene record:
CWH43 (80157)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011513759.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000414239 GATGCTTCTAAGCCCTATATG pLKO_005 981 CDS 100% 13.200 18.480 N CWH43 n/a
2 TRCN0000417738 GATTAGGACTACGGCATAAAG pLKO_005 799 CDS 100% 13.200 18.480 N CWH43 n/a
3 TRCN0000429495 CATGACCATTGCCATGATATT pLKO_005 524 CDS 100% 13.200 10.560 N CWH43 n/a
4 TRCN0000157296 CCCATGCTGAACTGAGTGATT pLKO.1 1522 CDS 100% 4.950 3.465 N CWH43 n/a
5 TRCN0000154775 GAACATGGCAATGTGAAGGAT pLKO.1 1422 CDS 100% 3.000 2.100 N CWH43 n/a
6 TRCN0000155952 CAAGAAGTTATTGGCTGGGAA pLKO.1 1748 3UTR 100% 2.640 1.848 N CWH43 n/a
7 TRCN0000154431 GCTTCCATCTTGTTTGTGGTT pLKO.1 329 CDS 100% 2.640 1.848 N CWH43 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011513759.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15164 pDONR223 93% 70.1% 33.8% None (many diffs) n/a
2 ccsbBroad304_15164 pLX_304 0% 70.1% 33.8% V5 (not translated due to prior stop codon) (many diffs) n/a
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