Transcript: Human NM_025087.3

Homo sapiens cell wall biogenesis 43 C-terminal homolog (CWH43), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-29
Taxon:
Homo sapiens (human)
Gene:
CWH43 (80157)
Length:
2444
CDS:
156..2255

Additional Resources:

NCBI RefSeq record:
NM_025087.3
NBCI Gene record:
CWH43 (80157)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_025087.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000156321 CCTGGGACCGATGATCTATTA pLKO.1 227 CDS 100% 13.200 18.480 N CWH43 n/a
2 TRCN0000414239 GATGCTTCTAAGCCCTATATG pLKO_005 1557 CDS 100% 13.200 18.480 N CWH43 n/a
3 TRCN0000417738 GATTAGGACTACGGCATAAAG pLKO_005 1375 CDS 100% 13.200 18.480 N CWH43 n/a
4 TRCN0000429495 CATGACCATTGCCATGATATT pLKO_005 1100 CDS 100% 13.200 10.560 N CWH43 n/a
5 TRCN0000157296 CCCATGCTGAACTGAGTGATT pLKO.1 2041 CDS 100% 4.950 3.465 N CWH43 n/a
6 TRCN0000156009 CCTGCTGAGGATAATCACTAT pLKO.1 368 CDS 100% 4.950 3.465 N CWH43 n/a
7 TRCN0000154775 GAACATGGCAATGTGAAGGAT pLKO.1 1941 CDS 100% 3.000 2.100 N CWH43 n/a
8 TRCN0000155952 CAAGAAGTTATTGGCTGGGAA pLKO.1 2267 3UTR 100% 2.640 1.848 N CWH43 n/a
9 TRCN0000154431 GCTTCCATCTTGTTTGTGGTT pLKO.1 905 CDS 100% 2.640 1.848 N CWH43 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_025087.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15164 pDONR223 93% 98.7% 61.3% None (many diffs) n/a
2 ccsbBroad304_15164 pLX_304 0% 98.7% 61.3% V5 (not translated due to prior stop codon) (many diffs) n/a
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