Construct: ORF ccsbBroad304_15174
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006107.1_s304c1, BRDN0000400114
- Derived from:
- ccsbBroadEn_15174
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM117A (81558)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15174
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81558 | FAM117A | family with sequence simila... | NM_030802.4 | 99.7% | 99.5% | 20_22delGCGinsNGA;510T>C |
| 2 | human | 81558 | FAM117A | family with sequence simila... | XM_017025182.1 | 84.9% | 79.4% | (many diffs) |
| 3 | mouse | 215512 | Fam117a | family with sequence simila... | NM_172543.4 | 87.4% | 87.4% | (many diffs) |
| 4 | mouse | 215512 | Fam117a | family with sequence simila... | XM_006532834.3 | 77.2% | 74.9% | (many diffs) |
| 5 | mouse | 215512 | Fam117a | family with sequence simila... | XM_006532835.2 | 76.2% | 74.1% | (many diffs) |
| 6 | mouse | 215512 | Fam117a | family with sequence simila... | XM_006532833.3 | 74.9% | 76.5% | (many diffs) |
| 7 | mouse | 215512 | Fam117a | family with sequence simila... | XM_006532836.2 | 59.8% | 58.9% | (many diffs) |
| 8 | mouse | 215512 | Fam117a | family with sequence simila... | XM_006532837.2 | 59.8% | 58.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1425
- ORF length:
- 1359
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaagg 61 ttggcatggc gggggccgca gcggngagca gaggcggagg tgcctggggg ccggggcgcg 121 gaggggccgg ggggctccgg cggggctgct ctcccccagc ccccgccggc tccccccggg 181 ctgggctgca gccgctcagg gccacgatcc ccttccagct gcagcagccg caccagcgcc 241 gggacggggg tggccgtgca gccagcgtcc catgctcggt ggccccagaa aagtcagtgt 301 gtaggcctca gccacttcag gtccggcgta cattctccct ggacaccatc ctcagctcct 361 accttctggg ccagtggcca cgagatgctg atggggcctt cacctgctgc accaatgaca 421 aggccaccca gacgcccctg tcctggcaag agctagaagg tgagcgtgcc agttcctgtg 481 cacacaagcg ctcagcatcc tggggcagca cagaccaccg aaaagagatt tccaagttga 541 agcaacaact gcagaggacg aagctgagcc gcagcgggaa agagaaggag cgaggttcac 601 cactcctagg ggaccacgca gtgcggggag cactgagggc gtcccctccc agcttcccct 661 cagggtcccc tgtcttgcga ctcagcccct gcctgcacag gagcctggaa gggctcaacc 721 aagagctgga ggaggtattt gtgaaggagc agggagaaga ggagctgctg aggatccttg 781 atatccctga tgggcaccgg gccccagctc ctccccagag tggcagctgt gatcatcccc 841 tcctcctcct ggagcctggc aaccttgcca gctctccttc catgtccttg gcatctcccc 901 agccttgtgg cctggccagt catgaggaac atcggggtgc cgccgaggag ctggcatcca 961 cccccaacga caaagcctcc tctccaggac acccagcctt tcttgaagat ggcagcccat 1021 ctccagtcct tgcctttgct gcctcccctc gacctaatca tagctacatc ttcaaacggg 1081 agcccccaga aggctgtgag aaagtgcgtg tgtttgaaga agccacgtct ccaggtcctg 1141 acctggcctt cctgacttcc tgtcctgaca agaacaaagt ccatttcaac ccgactggct 1201 cagccttctg ccccgtcaac ctgatgaagc ccctcttccc cggcatgggc ttcatcttcc 1261 gtaactgccc ctcaaacccg ggatctcccc ttcccccggc cagccccagg ccaccacctc 1321 ggaaggatcc ggaagcctcc aaggcctccc cactgccatt cgagccatgg cagcgcaccc 1381 caccatcaga agagcctgtg cttttccaga gctccctgat ggtctgccca accttcttgt 1441 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 1501 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a