Construct: ORF ccsbBroad304_15176

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016410.2_s304c1, BRDN0000459747
Derived from:
ccsbBroadEn_15176
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NUAK2 (81788)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15176
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 81788 NUAK2 NUAK family kinase 2 NM_030952.3 99.9% 100% 585T>C
2 human 81788 NUAK2 NUAK family kinase 2 XM_005245515.4 60.6% 60.6% 0_1ins741
3 mouse 74137 Nuak2 NUAK family, SNF1-like kina... NM_028778.4 83.7% 85.6% (many diffs)
4 mouse 74137 Nuak2 NUAK family, SNF1-like kina... NM_001195025.1 82.7% 84.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1950
ORF length:
1884
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga gtcgctggtt ttcgcgcggc gctccggccc cactccctcg gccgcagagc
121tagcccggcc gctggcggaa gggctgatca agtcgcccaa gcccctaatg aagaagcagg
181cggtgaagcg gcaccaccac aagcacaacc tgcggcaccg ctacgagttc ctggagaccc
241tgggcaaagg cacctacggg aaggtgaaga aggcgcggga gagctcgggg cgcctggtgg
301ccatcaagtc aatccggaag gacaaaatca aagatgagca agatctgatg cacatacgga
361gggagattga gatcatgtca tcactcaacc accctcacat cattgccatc catgaagtgt
421ttgagaacag cagcaagatc gtgatcgtca tggagtatgc cagccggggc gacctttatg
481actacatcag cgagcggcag cagctcagtg agcgcgaagc taggcatttc ttccggcaga
541tcgtctctgc cgtgcactat tgccatcaga acagagttgt ccaccgagat ctcaagctgg
601agaacatcct cttggatgcc aatgggaata tcaagattgc tgacttcggc ctctccaacc
661tctaccatca aggcaagttc ctgcagacat tctgtgggag ccccctctat gcctcgccag
721agattgtcaa tgggaagccc tacacaggcc cagaggtgga cagctggtcc ctgggtgttc
781tcctctacat cctggtgcat ggcaccatgc cctttgatgg gcatgaccat aagatcctag
841tgaaacagat cagcaacggg gcctaccggg agccacctaa accctctgat gcctgtggcc
901tgatccggtg gctgttgatg gtgaacccca cccgccgggc caccctggag gatgtggcca
961gtcactggtg ggtcaactgg ggctacgcca cccgagtggg agagcaggag gctccgcatg
1021agggtgggca ccctggcagt gactctgccc gcgcctccat ggctgactgg ctccggcgtt
1081cctcccgccc cctcctggag aatggggcca aggtgtgcag cttcttcaag cagcatgcac
1141ctggtggggg aagcaccacc cctggcctgg agcgccagca ttcgctcaag aagtcccgca
1201aggagaatga catggcccag tctctccaca gtgacacggc tgatgacact gcccatcgcc
1261ctggcaagag caacctcaag ctgccaaagg gcattctcaa gaagaaggtg tcagcctctg
1321cagaaggggt acaggaggac cctccggagc tcagcccaat ccctgcgagc ccagggcagg
1381ctgcccccct gctccccaag aagggcattc tcaagaagcc ccgacagcgc gagtctggct
1441actactcctc tcccgagccc agtgaatctg gggagctctt ggacgcaggc gacgtgtttg
1501tgagtgggga tcccaaggag cagaagcctc cgcaagcttc agggctgctc ctccatcgca
1561aaggcatcct caaactcaat ggcaagttct cccagacagc cttggagctc gcggccccca
1621ccaccttcgg ctccctggat gaactcgccc cacctcgccc cctggcccgg gccagccgac
1681cctcaggggc tgtgagcgag gacagcatcc tgtcctctga gtcctttgac cagctggact
1741tgcctgaacg gctcccagag cccccactgc ggggctgtgt gtctgtggac aacctcacgg
1801ggcttgagga gcccccctca gagggccctg gaagctgcct gaggcgctgg cggcaggatc
1861ctttggggga cagctgcttt tccctgacag actgccagga ggtgacagcg acctaccgac
1921aggcactgag ggtctgctca aagctcacct gcccaacttt cttgtacaaa gtggttggta
1981agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc
2041ggtggcgcgt taagtcgaca atcaacctct ggatta

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