Construct: ORF ccsbBroad304_15227
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018929.1_s304c1, BRDN0000459413
- Derived from:
- ccsbBroadEn_15227
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- ALPK2 (115701)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15227
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 115701 | ALPK2 | alpha kinase 2 | NM_052947.4 | 88.7% | 12.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 909
- ORF length:
- 840
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcatcaaag ttcacggatg gtgacctgaa caatgatggt cctcatgatg 121 aaggcttacg ctctagtcag caaaatccca aagtacagaa atacattagc ttcagcctcc 181 cgctgtctga ggcaactgca cacatttacc caggtgacag tgccgtggcc aacaaacaac 241 ccagcccaca gctttccagt gaagactctg acagtgacta tgaactttgc ccagagataa 301 ccctaaccta caccgaggag ttttcagatg atgacctgga gtatctggaa tgttctgatg 361 ttatgacgga ttactctaat gcagtttggc aaaggaacct gctggggact gagcatgttt 421 ttttattaga aagcgatgac gaagagatgg aattcggtga gcattgcctg ggtgggtgtg 481 agcatttcct cagtggaatg ggttgtgggt ctcgggtgtc gggtgacgct gggcctatgg 541 ttgccactgc tggcttctgt ggtcatcact cacaacccca agaagttggg gtgaggagca 601 gcagagtctc caagcacggt ccctcatccc cacaaacagg gatgactctc attttgggac 661 ctcaccagga tggaacgtct tcagtgacag aacaggggag atataaactc cccactgctc 721 ccgaggctgc tgaaaatgat tatccaggaa ttcaaggaga aaccagagac agccaccaag 781 caagagagga atttgccagt gacaatctgc tcaacatgga tgaatcagta agagagacag 841 agatgaagct ccttgtctgg tggagtcaga aaaacttcag ggatggagca gtgttgggag 901 acggcagctg acaagagagt ggggggaaag gacttatgga gcaagagggg ttcaaggaaa 961 tctgccaggg tgaggcagcc gggaatgaag ggaaatccca agaagccgaa tgccaacctg 1021 agagaaagta caacagaagg tacccttcat ctctgctctg ccaaagaatc tgctgagccc 1081 ccactaaccc agagtgataa aagagagact tctcacacca cagcagcagc gactggtcgg 1141 agttcccatg ctgatgcaag agaatgtgct atttcaaccc aggcagagca agaagcaaaa 1201 acccttcaaa cttcaacaga ctcagtctcc aaagaaggca acacaaattg caagggagaa 1261 ggcatgcaag ttaatactct atttgaaaca agccaggttc cagactggag tgatcctcct 1321 caggtacaag ttcaggaaac agtcagagag acaatctctt gcagccagat gccagctttc 1381 tcagagcctg ctggggagga gtccccattc actgggacca caacaatttc cttctcaaac 1441 ttaggagggg tccacaagga aaatgcatca ttagctcaac actcggaggt caaaccctgt 1501 acctgtggtc cacagcatga agaaaaacaa gacagagatg gcaacatacc tgacaatttc 1561 agggaagacc taaaatatga gcagagcatc tcagaagcca atgatgagac tatgtcccca 1621 ggtgtgttct caaggcatct ccccaaggat gctcgtgctg acttcaggga gcctgtggct 1681 gtctctgttg cttcccctga acccacagat actgccctca ccctggaaaa tgtgtgtgat 1741 gagccaaggg acagagaagc agtgtgtgca atggagtgtt ttgaggctgg tgaccaagga 1801 acgtgttttg ataccataga ttctcttgtt gggagaccag ttgataaata ttcgcctcaa 1861 gaaatttgct ctgtagatac ggaactggca gaaggtcaaa acaaagtatc tgatttatgt 1921 tcttctaatg acaagacact ggaagtcttt tttcagacac aagtgtctga gacttcagtg 1981 tctacgtgca aaagcagcaa ggacggcaac tcagtcatgt cccctctttt tatcagtact 2041 ttcaccttga acatttcaca cacagctagt gaaggtgcca caggagaaaa tctagccaag 2101 gtggagaatt ccacctaccc actggcctcc acagtacatg ctggccagga gcagccaagc 2161 cccagcaact caggagggct tgatgaaaca cagctccttt cttctgagaa caatccttta 2221 gtgcaattta aagaaggagg tgacaagagc cccagtccta gtgccgcaga caccacagcc 2281 acaccagcca gttatagttc aattgtgagt tttccttggg agaagccaac aacattaact 2341 gctaataatg agtgctttca agcgaccagg gagactgagg acacatcaac tgttaccatt 2401 gccaccgaag tccacccagc caaatacctt gctgtgtcaa ttcctgagga caagcatgca 2461 ggtggcactg aggagaggtt ccctcgtgca tcccatgaaa aggtttccca atttccttcc 2521 caagtgcagg tggatcatat tttaagtggt gctaccatca aatctacaaa agagctactt 2581 tgcagggcac ccagtgtgcc aggagtccca caccatgtcc tgcagctccc agagggagag 2641 ggtttctgca gtaattcccc tcttcaggtt gataacctgt ctggagataa gagccagact 2701 gtggacagag cagactttag gagctatgaa gagaatttcc aagaaagagg aagtgaaaca 2761 aagcaggggg tccagcagca gagcctgtcc cagcagggtt ctctttctgc acctgatttc 2821 caacaaagtt tgcctacgac atctgctgca caagaggaaa gaaacttggt gcccacggcc 2881 ccctcaccag caagctctag ggaaggagca gggcagcgct caggttgggg gacgagggtc 2941 tccgtggtgg ctgaaactgc tggggaagaa gacagtcagg ctctgagcaa cgttccatct 3001 ctctctgata tccttttgga agagtctaaa gaatatagac ctggaaattg ggaggcaggc 3061 aacaagctga agattataac tctagaggct tccgcttctg aaatctggcc accacgacaa 3121 ctgacaaatt ctgagagcaa ggcatcagac ggtggtctca taattcctga caaggtctgg 3181 gctgtacctg atagtctaaa ggcagatgct gttgtgcctg aattggcccc ctctgaaata 3241 gcagcattgg ctcacagtcc agaggatgct gagtcagccc ttgctgatag cagagaaagc 3301 cataaaggcg aagagcccac catcagtgta cactggagaa gtctttcttc ccggggtttc 3361 agccaaccga gactcctgga gtcatccgtg gaccctgtag atgaaaagga gttatctgtc 3421 acagattcac tgtcagcggc ttctgaaact ggagggaagg aaaatgttaa caatgtgagt 3481 caagaccagg aggaaaaaca actcaagatg gatcacactg ccttctttaa aaagtttctg 3541 acctgcccta aaatcctaga gtcctctgta gatcccattg atgagataag tgtgatagag 3601 tacaccaggg ctggaaaacc agagccctct gaaaccacac cacagggcgc cagagaaggg 3661 ggtcaatcaa atgacggaaa catgggccac gaagcggaaa tccagccggc cattttgcaa 3721 gttccatgtc tccagggaac cattctgagt gaaaatagaa tcagcagaag ccaagaaggc 3781 agtatgaagc aggaggcaga acaaattcaa cctgaggagg caaaaactgc catttggcaa 3841 gtcctgcaac ccagcgaagg cggtgaaaga attccaagtg gatgtagcat aggccaaata 3901 caagaaagca gtgatgggag cttaggggag gctgagcaaa gcaaaaagga caaagcagaa 3961 ttgatttccc ccacttcacc tctttctagt tgtcttccaa taatgactca cgcttctctt 4021 ggggttgaca cgcacaactc cacaggccaa attcatgacg tccctgaaaa tgacatagtt 4081 gagcccagaa agcgtcagta tgtgtttcct gtttcacaga aaaggggaac tattgagaat 4141 gagcgtggga aacctttgcc ctcttctcct gatcttacca ggttcccttg tacttcatct 4201 cctgaaggaa atgtcacaga ctttttgata agccacaaaa tggaggaacc taaaatagag 4261 gtgcttcaaa ttggggaaac caaaccccca agctcatcta gctcctcagc gaagaccttg 4321 gcatttattt caggagaacg tgagttagag aaagccccta agttactgca ggatccatgt 4381 caaaagggca ccctgggctg tgcgaaaaag tccagggaga gagagaagtc cctggaagcc 4441 cgagcaggca aatcgccagg gaccctcaca gcagtgacgg ggtcagagga ggtcaagagg 4501 aagccagaag ccccaggcag tggacattta gctgagggag taaagaagaa aattttgtcc 4561 agggtggcag cactgaggct gaaactggaa gaaaaggaaa atatcagaaa gaactcagcc 4621 tttcttaaaa agatgcccaa actcgaaaca tcattatcac acacagaaga gaaacaagac 4681 ccaaaaaagc catcttgcaa aagagaagga agagctccag tattactgaa aaagatccaa 4741 gctgagatgt tccctgaaca ctctggaaat gtaaaattaa gctgccaatt tgcagaaatt 4801 catgaagatt ctactatctg ctggacaaaa gattcaaagt ccatagccca agtgcagaga 4861 agtgcagggg acaactccac tgtttccttt gccatcgtgc aagccagtcc gaaggaccag 4921 ggactctatt actgctgcat caagaacagc tacggaaaag tgactgctga atttaacctc 4981 acagctgaag ttctcaaaca gctgtcaagt cgccaggata ctaaaggatg tgaagagatt 5041 gaattcagcc aactcatctt caaagaagac ttcctccatg acagctactt ttggggccgc 5101 ctgcgtggtc agatcgccac ggaggagctg cactnngaga angggttcac cgcaaagctt 5161 tcngcagcac agtgatgcac ggcttcatgc ctgtcttcaa acctggccat gcctgtgtgc 5221 ttaaggtgca caatgccatt gcctatggga ccagaaataa tgatgagctc atccaaagga 5281 actacaaact cgctgcccag gaatgctatg ttcaaaatac tgccaggtat tatgccaaga 5341 tctacgctgc tgaagcacag cctctggaag gctttggaga agtacctgag atcattccta 5401 tttttcttat ccatcggcct gagaacaata tcccgtatgc tacagtggag gaggagctga 5461 ttggagaatt tgtgaagtat tccatcaggg atgggaaaga aataaacttc ttgagaagag 5521 aatcagaagc tggtcagaaa tgttgcacct tccagcactg ggtgtaccag aaaacaagtg 5581 gctgcctcct ggtgacggac atgcaaggtg taggaatgaa gctaactgac gttggcatag 5641 caacgctggc taaagggtac aagggattta aaggcaactg ttccatgacc ttcattgatc 5701 agtttaaagc actacaccag tgtaacaagt attgcaaaat gctgggactg aaatcccttc 5761 aaaacaacaa ccagaaacag aagcagccga gcattgggaa aagcaaagtt caaacaaact 5821 ctatgacagt aaagaaggca gggcctgaga ccccaggcga aaagaaaacc ttgccaactt 5881 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 5941 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta