Construct: ORF ccsbBroad304_15248
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017457.1_s304c1, BRDN0000399483
- Derived from:
- ccsbBroadEn_15248
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- NDUFAF6 (137682)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15248
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 137682 | NDUFAF6 | NADH:ubiquinone oxidoreduct... | NM_152416.4 | 34.9% | 7% | (many diffs) |
| 2 | human | 137682 | NDUFAF6 | NADH:ubiquinone oxidoreduct... | NR_148910.1 | 20% | (many diffs) | |
| 3 | human | 137682 | NDUFAF6 | NADH:ubiquinone oxidoreduct... | NR_148912.1 | 18.1% | (many diffs) | |
| 4 | human | 137682 | NDUFAF6 | NADH:ubiquinone oxidoreduct... | NR_148911.1 | 18% | (many diffs) | |
| 5 | human | 137682 | NDUFAF6 | NADH:ubiquinone oxidoreduct... | NR_148915.1 | 15.6% | (many diffs) | |
| 6 | human | 137682 | NDUFAF6 | NADH:ubiquinone oxidoreduct... | NR_148913.2 | 10% | (many diffs) | |
| 7 | human | 137682 | NDUFAF6 | NADH:ubiquinone oxidoreduct... | NR_148914.2 | 9.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 333
- ORF length:
- 267
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcctcgtgc acggctctgt ctgggggccg ttgcggcttg gcatccccgg 121 cctgtgctgc cgccggccgc ctctgggtct gtacgcgcgc atgcggcggc tgcccgggcc 181 ggaggtgtct gggcggagcg tggctgcggc cagcggaccg ggcgcctggg gcactgacca 241 ctactgcctg gagctgctgc ggaaacggga ttatgaaggt tatttatgct ccctgctgct 301 ccctgcagaa tcccgaagct ctgtttttgc actgagggcc tttaatgtgg aactggctca 361 ggctggatta ctcttgctgc tgtcttgctg tactgtatgc cactgggatc tgaacactaa 421 acattgcaac ccaactttct tgtacaaagt ggttggtaag cctatcccta accctctcct 481 cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta agtcgacaat 541 caacctctgg atta