Construct: ORF ccsbBroad304_15250
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002089.1_s304c1, BRDN0000462414
- Derived from:
- ccsbBroadEn_15250
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DGKK (139189)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15250
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 139189 | DGKK | diacylglycerol kinase kappa | NM_001013742.4 | 100% | 100% | |
| 2 | human | 139189 | DGKK | diacylglycerol kinase kappa | XM_017029268.2 | 97.7% | 97.7% | 3414_3415ins87 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3882
- ORF length:
- 3813
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaccgcgga gctgccgcag cccagggcac tgccccgcct caggatggag 121 agcagcccgc tgagtctcca gagcctccgc cgccttggcc gccgccgcca ccaccaccgg 181 ctccgccgcc ggctccgccg ctgctctccg aggcttcgcc agaacccata ccagagccct 241 gtccagagct tgctccaggt ccctgtccag aggcgacctc agaatcagcc acagaactgt 301 acacagaacc gaccccagaa ccagccacag agccggcctc agaaccggcc ccagaacctg 361 ccacagagcc ggccccagaa cctgccacag agccggcccc agaaccggcc ccagaacctg 421 ccacagagtc tgccccagag ccgactccag aacctgccct agagtcggtc ccagagccgg 481 ccccagagct gactccagaa gttgccccag agctggcccc agagccgacc ccagaacctg 541 tgacagagct ggccccagag ttctgccctg aggcggctcc agagttccgt ccaagtccag 601 caccatgtct attgcaatgt ccggtggaca ctcgagagag aggtctaaag acctcgccat 661 cgccatcgcc atcgccatcg cccagaacgc caatgtcgtg gtccagaata aagaaaatat 721 tgaaggaagg acctatgctg aagaactgta actctttcaa gagatggaag cttagatatt 781 ttctggttca aggacagaag ctctactttg cacaccatcc cgcgtttgca cactttgaaa 841 cgattgatct gtctcaagcc actgtggcag aaagcagctg tagaaacctt tgccacagtt 901 tttgtgttat tacaccacaa cgaaaaatca ctctggctgc acccaaccgg aaagacatgg 961 aagaatggat taacatcata aaaaccatcc aacagggaga aatttataag atacctgcag 1021 cagaaaacaa cccttttctt gttggaatgc attgttggta ctccagttac agccaccgga 1081 cccagcactg caatgtttgt cgagagagca ttcctgcctt atctagagat gccatcatct 1141 gtgaagtgtg caaagtgaaa tctcacagat tgtgtgcttt gagagcaagc aaagactgca 1201 agtggaatac attgtctatc actgatgacc tccttctgcc tgcagatgaa gtaaacatgc 1261 cccatcaatg ggtagaagga aacatgcctg tcagctctca gtgtgcagtg tgtcatgaga 1321 gctgtggcag ttatcaaaga cttcaagact tccgctgcct gtggtgtaat tctacggtgc 1381 atgatgactg taggagacgg ttttccaagg aatgttgctt cagaagccat cgctcatcag 1441 tcattcctcc cactgctcta agcgacccca aaggcgatgg ccaattagta gtatcttcag 1501 acttctggaa tcttgattgg tcatcagcct gttcatgtcc cttgctcatc ttcatcaact 1561 ccaaaagtgg cgatcatcag gggatcgtct tcctccgaaa attcaagcaa taccttaacc 1621 catctcaagt gttcgactta ttgaagggtg gacctgaagc agggctgtct atgttcaaga 1681 actttgctcg ctttcgcatt ctggtttgtg gtggagatgg cagcgtgagc tgggtcttat 1741 ctctgattga tgcctttgga ttacatgaaa agtgtcagtt ggcagtcatc ccacttggaa 1801 ccggcaatga tctggctcgt gttctgggct ggggtgcatt ctggaacaaa agcaagtcac 1861 ctctggacat cctcaacaga gtggagcagg ctagtgtgag gatcctagac agatggagtg 1921 tgatgattcg tgagactccc agacaaaccc cgctgctaaa aggacaggtt gaaatggatg 1981 taccacgatt tgaggctgct gccatccaac acttagaatc tgcagccacc gagttgaaca 2041 aaatcctgaa ggccaagtac cccacagaga tgatcatcgc aaccagattc ttgtgttcag 2101 ctgtggaaga ttttgtggtt gatattgtaa aggcctgggg tcagataaaa cagaacaaca 2161 ctgcaatagt gtctgtgatt ttgaaaagtg acttaatgta tgataggctc agtgtcctga 2221 tcgatgtcct ggctgaggag gcagcagcta cttctgctga aaaaagtgct acagaatatg 2281 cagacagcag caaggcagat aggaagccct tcattcctca aatagaccac atagccaagt 2341 gcaagttgga gctggctaca aaggcccaga gtctccagaa atccttgaaa ctcatcatat 2401 ttcaagttga acaagctctg gatgaggaaa gcagacagac aatatctgtt aagaacttta 2461 gttcaacttt cttcctggaa gatgacccag aagatattaa ccagacaagc ccacgacgcc 2521 gttctcgtcg tggcactttg tcttctatat cttctctcaa aagtgaggac ctggacaacc 2581 ttaacttgga tcacttacat tttacacctg aatctatacg cttcaaagaa aaatgtgtca 2641 tgaacaacta cttcggaatt ggactggatg ctaaaatttc tctggacttc aacaccagaa 2701 gagatgaaca cccagggcaa tacaatagcc gccttaagaa caagatgtgg tatggccttc 2761 tgggaaccaa agaacttttg cagcgctctt acaggaaact ggaagaacga gtgcatttgg 2821 agtgtgatgg agaaaccatc tccttgccaa acctgcaagg cattgtagtg ctcaacatta 2881 ccagctatgc tggaggtatc aacttctggg gaagcaacac agcaaccacg gaatatgagg 2941 ctcctgcaat cgatgatggg aaactggagg tggtggcaat ctttggttct gtgcagatgg 3001 caatgtcccg tatcatcaac ctgcatcatc atcgcattgc ccagtgccat gaggtgatga 3061 taaccattga tggtgaagaa ggtatcccag tgcaggtgga tggggaggcc tggattcaga 3121 gaccaggcct tatcaaaatt agatacaaga acgctgccca gatgctgaca agagatcggg 3181 actttgagaa ctcaatgaaa atgtgggaat acaagcatac tgaaattcaa gctgcccctc 3241 aaccccagct ggacttccag gactctcaag agagcctctc tgacgaggag tatgcccaga 3301 tgcagcactt agctcggctt gcagaaaacc tcatcagcaa acttaatgac ctgagcaaga 3361 tccaccagca tgtgtctgtc ctcatgggtt ctgtgaatgc cagcgctaac atcctgaatg 3421 atatatttta cggccaagac agtggcaatg agatgggtgc agcttcctgt attcccattg 3481 aaactctaag cagaaatgat gccgtagatg ttacatttag tcttaaaggt ctctacgatg 3541 acaccacagc tttcctggat gaaaagctgc tgagaagtgc tgaggatgag actgcactac 3601 aaagcgccct ggatgccatg aataaggagt tcaaaaagct atctgagatt gactggatga 3661 atccaatctt tgttccagag gaaaaatctt cggacactga cagtagaagc ctcaggctga 3721 aaattaagtt ccccaaattg ggaaagaaaa aggtagaaga ggaacgcaag cctaaatcag 3781 gccagagtgt ccagagtttt attggcaatt tatggcaccg cagacatcgt gaagatgaag 3841 cagagggtga tgatcctcta acaccatcga gatctcaact gttgccaact ttcttgtaca 3901 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 3961 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta