Transcript: Human NM_001013742.4

Homo sapiens diacylglycerol kinase kappa (DGKK), mRNA.

Source:
NCBI, updated 2019-08-22
Taxon:
Homo sapiens (human)
Gene:
DGKK (139189)
Length:
7494
CDS:
148..3963

Additional Resources:

NCBI RefSeq record:
NM_001013742.4
NBCI Gene record:
DGKK (139189)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146124 AATATGCTATACATTACCGC pXPR_003 GGG 756 20% 2 1.0448 DGKK DGKK 75844
2 BRDN0001144811 GTGTTCGACTTATTGAAGGG pXPR_003 TGG 1577 41% 9 0.3637 DGKK DGKK 75842
3 BRDN0001145466 ACCGGACATTGCAATAGACA pXPR_003 TGG 540 14% 1 0.2451 DGKK DGKK 75843
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001013742.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000360253 CATCTCAAGTGTTCGACTTAT pLKO_005 1700 CDS 100% 13.200 18.480 N DGKK n/a
2 TRCN0000360254 TCCGCTGCCTGTGGTGTAATT pLKO_005 1430 CDS 100% 13.200 18.480 N DGKK n/a
3 TRCN0000052546 GCAATACAATAGCCGCCTTAA pLKO.1 2796 CDS 100% 10.800 15.120 N DGKK n/a
4 TRCN0000052543 CCTGAATCTATACGCTTCAAA pLKO.1 2686 CDS 100% 5.625 7.875 N DGKK n/a
5 TRCN0000052544 GCCATGAATAAGGAGTTCAAA pLKO.1 3694 CDS 100% 5.625 3.938 N DGKK n/a
6 TRCN0000052545 CCATCTCAAGTGTTCGACTTA pLKO.1 1699 CDS 100% 4.950 3.465 N DGKK n/a
7 TRCN0000052547 GCATTGTTGGTACTCCAGTTA pLKO.1 1128 CDS 100% 4.950 3.465 N DGKK n/a
8 TRCN0000360252 ACCTTAACTTGGATCACTTAC pLKO_005 2657 CDS 100% 10.800 6.480 N DGKK n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001013742.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15250 pDONR223 63% 100% 100% None n/a
2 ccsbBroad304_15250 pLX_304 0% 100% 100% V5 n/a
3 ccsbBroadEn_15251 pDONR223 43.5% 99.5% 18.5% None (many diffs) n/a
4 ccsbBroad304_15251 pLX_304 0% 99.5% 18.5% V5 (not translated due to prior stop codon) (many diffs) n/a
5 TRCN0000470266 TGAGCCCCTGCAGCTATTTTACCA pLX_317 10.8% 99.5% 18.5% V5 (not translated due to prior stop codon) (many diffs) n/a
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