Construct: ORF ccsbBroad304_15327

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004110.2_s304c1, BRDN0000459756
Derived from:
ccsbBroadEn_15327
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EIF2AK4 (440275)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15327
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 440275 EIF2AK4 eukaryotic translation init... NM_001013703.4 48.8% 48.8% 1_2529del;3916G>T
2 mouse 27103 Eif2ak4 eukaryotic translation init... XM_017318915.1 48.8% 51.6% (many diffs)
3 mouse 27103 Eif2ak4 eukaryotic translation init... XM_006499627.3 47.5% 50.2% (many diffs)
4 mouse 27103 Eif2ak4 eukaryotic translation init... XM_006499626.3 46.7% 49.3% (many diffs)
5 mouse 27103 Eif2ak4 eukaryotic translation init... NM_001177806.1 46.4% 49% (many diffs)
6 mouse 27103 Eif2ak4 eukaryotic translation init... XM_006499625.3 46.4% 49% (many diffs)
7 mouse 27103 Eif2ak4 eukaryotic translation init... XM_017318909.1 43.9% 46.4% (many diffs)
8 mouse 27103 Eif2ak4 eukaryotic translation init... NM_013719.3 43.2% 45.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2484
ORF length:
2418
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgat tcaccgggat ttgaagcctg tcaacatttt tttggattct gatgaccatg
121tgaaaatagg tgattttggt ttggcgacag accatctagc cttttctgct gacagcaaac
181aagacgatca gacaggagac ttgattaagt cagacccttc aggtcactta actgggatgg
241ttggcactgc tctctatgta agcccagagg tccaaggaag caccaaatct gcatacaacc
301agaaagtgga tctcttcagc ctgggaatta tcttctttga gatgtcctat caccccatgg
361tcacggcttc agaaaggatc tttgttctca accaactcag agatcccact tcgcctaagt
421ttccagaaga ctttgacgat ggagagcatg caaagcagaa atcagtcatc tcctggctgt
481tgaaccacga tccagcaaaa cggcccacag ccacagaact gctcaagagt gagctgctgc
541ccccacccca gatggaggag tcagagctgc atgaagtgct gcaccacacg ctgaccaacg
601tggatgggaa ggcctaccgc accatgatgg cccagatctt ctcgcagcgc atctcccctg
661ccatcgatta cacctatgac agcgacatac tgaagggcaa cttctcaatc cgtacagcca
721agatgcagca gcatgtgtgt gaaaccatca tccgcatctt taaaagacat ggagctgttc
781agttgtgtac tccactactg cttccccgaa acagacaaat atatgagcac aacgaagctg
841ccctattcat ggaccacagc gggatgctgg tgatgcttcc ttttgacctg cggatccctt
901ttgcaagata tgtggcaaga aataatatat tgaatttaaa acgatactgc atagaacgtg
961tgttcaggcc gcgcaagtta gatcgatttc atcccaaaga acttctggag tgtgcatttg
1021atattgtcac ttctaccacc aacagctttc tgcccactgc tgaaattatc tacactatct
1081atgaaatcat ccaagagttt ccagcacttc aggaaagaaa ttacagtatt tatttgaacc
1141ataccatgtt attgaaagca atactcttac actgtgggat cccagaagat aaactcagtc
1201aagtctacat tattctgtat gatgctgtga cagagaagct gacgaggaga gaagtggaag
1261ctaaattttg taatctgtct ttgtcttcta atagtctgtg tcgactctac aagtttattg
1321aacagaaggg agatttgcaa gatcttatgc caacaataaa ttcattaata aaacagaaaa
1381caggtattgc acagttggtg aagtatggct taaaagacct agaggaggtt gttggactgt
1441tgaagaaact ctgcatcaag ttacaggtct tgatcaattt gggcttggtt tacaaggtgc
1501agcagcacaa tggaatcatc ttccagtttg tggctttcat caaacgaagg caaagggctg
1561tacctgaaat cctcgcagct ggaggcagat atgacctgct gattccccag tttagagggc
1621cacaagctct ggggccagtt cccactgcca ttggggtcag catagctata gacaagatat
1681ctgctgctgt cctcaacatg gaggaatctg ttacaataag ctcttgtgac ctcctggttg
1741taagtgttgg ccagatgtct atgtccaggg ccatcaacct aacccagaaa ctctggacag
1801caggcatcac agcagaaatc atgtacgact ggtcacagtc ccaagaggaa ttacaagagt
1861actgcagaca tcatgaaatc acctatgtgg cccttgtctc ggataaagaa ggaagccatg
1921tcaaggttaa gtctttcgag aaggaaaggc agacagagaa gcgtgtgctg gagactgaac
1981ttgtggacca tgtactgcag aaactgagga ctaaagtcac tgatgaaagg aatggcagag
2041aagcttccga taatcttgca gtgcaaaatc tgaaggggtc attttctaat gcttcaggtt
2101tgtttgaaat ccatggagca acagtggttc ccattgtgag tgtgctagcc ccggagaagc
2161tgtcagccag cactaggagg cgctatgaaa ctcaggtaca aactcgactt cagacctccc
2221ttgccaactt acatcagaaa agcagtgaaa ttgaaattct ggctgtggat ctacccaaag
2281aaacaatatt acagttttta tcattagagt gggatgctga tgaacaggca tttaacacaa
2341ctgtgaagca gctgctgtca cgcctgccaa agcaaagata cctcaaatta gtctgtgatg
2401aaatttataa catcaaagta gaaaaaaagg tgtctgtgct atttctgtac agctatagag
2461atgactacta cagaatctta ttttacccaa ctttcttgta caaagtggtt ggtaagccta
2521tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc
2581gcgttaagtc gacaatcaac ctctggatta

Download FASTA (ORF) (Full)