Construct: ORF ccsbBroad304_15370
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017519.1_s304c1, BRDN0000406083
- Derived from:
- ccsbBroadEn_15370
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PTTG1IP (754)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15370
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 754 | PTTG1IP | PTTG1 interacting protein | NM_004339.4 | 99.8% | 100% | 84G>A |
| 2 | human | 754 | PTTG1IP | PTTG1 interacting protein | NR_104597.1 | 15.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 606
- ORF length:
- 540
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc gcccggagtg gcccgcgggc cgacgccgta ctggaggttg cgcctcggtg 121 gcgccgcgct gctcctgctg ctcatcccag tggccgccgc gcaggagcct cccggagctg 181 cttgttctca gaacacaaac aaaacctgtg aagagtgcct gaagaacgtc tcctgtcttt 241 ggtgcaacac taacaaggct tgtctggact acccagttac aagcgtcttg ccaccggctt 301 ccctttgtaa attgagctct gcacgctggg gagtttgttg ggtgaacttt gaggcgctga 361 tcatcaccat gtcggtagtc gggggaaccc tcctcctggg cattgccatc tgctgctgct 421 gctgctgcag gaggaagagg agccggaagc cggacaggag tgaggagaag gccatgcgtg 481 agcgggagga gaggcggata cggcaggagg aacggagagc agagatgaag acaagacatg 541 atgaaatcag aaaaaaatat ggcctgttta aagaagaaaa cccgtatgct agatttgaaa 601 acaactnncc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 661 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 721 acctctggat ta