Construct: ORF ccsbBroad304_15377

Construct Description:

• Construct Type: ORF
• Other Identifiers: ORF001394.1_s304c1, BRDN0000405648
• Derived from: ccsbBroadEn_15377
• DNA Barcode: None
• Epitope Tag: V5
• Notes: No stop codon in insert

Originally Annotated References:

• Gene: CBS (875)

Vector Information:

• Vector Backbone: pLX_304
• Pol II Cassette 1: PGK-BlastR
• Pol II Cassette 2: CMV-ccsbBroad304_15377
• Selection Marker: BlastR
• Visible Reporter: n/a
• Epitope Tag: V5

Current transcripts matched by this ORF:

	Taxon	Gene	Symbol	Description	Transcript	Nuc. Match %	Prot. Match %	Match Diffs
1	human	875	CBS	cystathionine beta-synthase	NM_000071.2	99.9%	99.8%	205G>C
2	human	875	CBS	cystathionine beta-synthase	NM_001178008.2	99.9%	99.8%	205G>C
3	human	875	CBS	cystathionine beta-synthase	NM_001178009.3	99.9%	99.8%	205G>C
4	human	875	CBS	cystathionine beta-synthase	NM_001320298.1	99.9%	99.8%	205G>C
5	human	875	CBS	cystathionine beta-synthase	XM_017028491.2	99.9%	99.8%	205G>C
6	human	102724560	CBSL	cystathionine beta-synthase...	NM_001321073.2	99.8%	99.8%	205G>C;699T>C
7	human	102724560	CBSL	cystathionine beta-synthase...	NM_001354006.1	99.8%	99.8%	205G>C;699T>C
8	human	102724560	CBSL	cystathionine beta-synthase...	NM_001354007.1	99.8%	99.8%	205G>C;699T>C
9	human	102724560	CBSL	cystathionine beta-synthase...	NM_001354008.1	99.8%	99.8%	205G>C;699T>C
10	human	102724560	CBSL	cystathionine beta-synthase...	NM_001354009.1	99.8%	99.8%	205G>C;699T>C
11	human	102724560	CBSL	cystathionine beta-synthase...	NM_001354010.1	99.8%	99.8%	205G>C;699T>C
12	human	102724560	CBSL	cystathionine beta-synthase...	NM_001354012.1	99.8%	99.8%	205G>C;699T>C
13	human	875	CBS	cystathionine beta-synthase	XM_011529777.2	97.4%	97.1%	205G>C;1553_1594del
14	human	102724560	CBSL	cystathionine beta-synthase...	NM_001354014.1	97.4%	97.1%	205G>C;699T>C;1553_1594del
15	human	102724560	CBSL	cystathionine beta-synthase...	NM_001354015.1	97.4%	97.1%	205G>C;699T>C;1553_1594del
16	human	875	CBS	cystathionine beta-synthase	XM_024452137.1	85.3%	77.8%	(many diffs)
17	human	102724560	CBSL	cystathionine beta-synthase...	XM_017028209.1	85.1%	77.8%	(many diffs)
18	human	102724560	CBSL	cystathionine beta-synthase...	XM_017028210.1	85.1%	77.8%	(many diffs)
19	human	875	CBS	cystathionine beta-synthase	XM_011529774.2	83.3%	75.9%	(many diffs)
20	human	875	CBS	cystathionine beta-synthase	XM_024452136.1	83.3%	75.9%	(many diffs)
21	human	875	CBS	cystathionine beta-synthase	NM_001321072.1	80.8%	80.7%	1_1delAins316
22	human	875	CBS	cystathionine beta-synthase	XM_024452138.1	80.8%	80.7%	1_1delAins316
23	human	875	CBS	cystathionine beta-synthase	XM_024452139.1	80.8%	80.7%	1_1delAins316
24	human	875	CBS	cystathionine beta-synthase	XM_024452140.1	80.8%	80.7%	1_1delAins316
25	human	875	CBS	cystathionine beta-synthase	XR_001754917.2	80.8%		(many diffs)
26	human	875	CBS	cystathionine beta-synthase	XM_011529783.2	78.8%	78.5%	1_1delAins316;1238_1279del
27	human	875	CBS	cystathionine beta-synthase	XR_001754916.2	67.8%		(many diffs)
28	human	102724560	CBSL	cystathionine beta-synthase...	NR_148682.1	60.6%		(many diffs)
29	human	875	CBS	cystathionine beta-synthase	XR_001754915.1	53.1%		(many diffs)
30	human	875	CBS	cystathionine beta-synthase	XR_002958634.1	47.8%		(many diffs)

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:

66

ORF end:

1719

ORF length:

1653

Sequence:

1	ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61	ttggcatgcc ttctgagacc ccccaggcag aagtggggcc cacaggctgc ccccaccgct
121	cagggccaca ctcggcgaag gggagcctgg agaaggggtc cccagaggat aaggaagcca
181	aggagcccct gtggatccgg cccgatgctc cgagcaggtg cacctggcag ctgggccggc
241	ctgcctccga gtccccacat caccacactc ccccggcaaa atctccaaaa atcttgccag
301	atattctgaa gaaaatcggg gacaccccta tggtcagaat caacaagatt gggaagaagt
361	tcggcctgaa gtgtgagctc ttggccaagt gtgagttctt caacgcgggc gggagcgtga
421	aggaccgcat cagcctgcgg atgattgagg atgctgagcg cgacgggacg ctgaagcccg
481	gggacacgat tatcgagccg acatccggga acaccgggat cgggctggcc ctggctgcgg
541	cagtgagggg ctatcgctgc atcatcgtga tgccagagaa gatgagctcc gagaaggtgg
601	acgtgctgcg ggcactgggg gctgagattg tgaggacgcc caccaatgcc aggttcgact
661	ccccggagtc acacgtgggg gtggcctggc ggctgaagaa cgaaatcccc aattctcaca
721	tcctagacca gtaccgcaac gccagcaacc ccctggctca ctacgacacc accgctgatg
781	agatcctgca gcagtgtgat gggaagctgg acatgctggt ggcttcagtg ggcacgggcg
841	gcaccatcac gggcattgcc aggaagctga aggagaagtg tcctggatgc aggatcattg
901	gggtggatcc cgaagggtcc atcctcgcag agccggagga gctgaaccag acggagcaga
961	caacctacga ggtggaaggg atcggctacg acttcatccc cacggtgctg gacaggacgg
1021	tggtggacaa gtggttcaag agcaacgatg aggaggcgtt cacctttgcc cgcatgctga
1081	tcgcgcaaga ggggctgctg tgcggtggca gtgctggcag cacggtggcg gtggccgtga
1141	aggccgcgca ggagctgcag gagggccagc gctgcgtggt cattctgccc gactcagtgc
1201	ggaactacat gaccaagttc ctgagcgaca ggtggatgct gcagaagggc tttctgaagg
1261	aggaggacct cacggagaag aagccctggt ggtggcacct ccgtgttcag gagctgggcc
1321	tgtcagcccc gctgaccgtg ctcccgacca tcacctgtgg gcacaccatc gagatcctcc
1381	gggagaaggg cttcgaccag gcgcccgtgg tggatgaggc gggggtaatc ctgggaatgg
1441	tgacgcttgg gaacatgctc tcgtccctgc ttgccgggaa ggtgcagccg tcagaccaag
1501	ttggcaaagt catctacaag cagttcaaac agatccgcct cacggacacg ctgggcaggc
1561	tctcgcacat cctggagatg gaccacttcg ccctggtggt gcacgagcag atccagtacc
1621	acagcaccgg gaagtccagt cagcggcaga tggtgttcgg ggtggtcacc gccattgact
1681	tgctgaactt cgtggccgcc caggagcggg accagaagtn nccaactttc ttgtacaaag
1741	tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta
1801	gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta

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