Construct: ORF ccsbBroad304_15502
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009324.1_s304c1, BRDN0000408784
- Derived from:
- ccsbBroadEn_15502
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MTRR (4552)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15502
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4552 | MTRR | 5-methyltetrahydrofolate-ho... | NM_001364440.2 | 100% | 100% | |
| 2 | human | 4552 | MTRR | 5-methyltetrahydrofolate-ho... | NM_001364441.2 | 100% | 100% | |
| 3 | human | 4552 | MTRR | 5-methyltetrahydrofolate-ho... | NM_001364442.2 | 100% | 100% | |
| 4 | human | 4552 | MTRR | 5-methyltetrahydrofolate-ho... | NM_002454.3 | 100% | 100% | |
| 5 | human | 4552 | MTRR | 5-methyltetrahydrofolate-ho... | NM_024010.4 | 100% | 100% | |
| 6 | human | 4552 | MTRR | 5-methyltetrahydrofolate-ho... | XM_024446064.1 | 100% | 100% | |
| 7 | human | 4552 | MTRR | 5-methyltetrahydrofolate-ho... | XM_024446063.1 | 97.8% | 97.8% | 1_45del |
| 8 | human | 4552 | MTRR | 5-methyltetrahydrofolate-ho... | NR_157168.2 | 64.6% | 1_67del;335_336insGGTATGGGTTACTG;2148_3205del | |
| 9 | human | 4552 | MTRR | 5-methyltetrahydrofolate-ho... | NR_157173.2 | 64.5% | 1_67del;1212_1234del;2185_3242del | |
| 10 | human | 4552 | MTRR | 5-methyltetrahydrofolate-ho... | NR_157177.2 | 64.3% | 1_93del;493_494insGTTTA;2183_3240del | |
| 11 | human | 4552 | MTRR | 5-methyltetrahydrofolate-ho... | NR_134480.2 | 64% | 1_93del;361_362insGGTATGGGTTACTG;2174_3231del | |
| 12 | human | 4552 | MTRR | 5-methyltetrahydrofolate-ho... | NR_157178.2 | 64% | 1_93del;1238_1260del;2211_3268del | |
| 13 | human | 4552 | MTRR | 5-methyltetrahydrofolate-ho... | NR_134481.2 | 61.7% | 1_93del;1146_1147ins89;2099_3156del | |
| 14 | human | 4552 | MTRR | 5-methyltetrahydrofolate-ho... | NR_157175.2 | 61.6% | 1_93del;1420_1571del;2340_3397del | |
| 15 | human | 4552 | MTRR | 5-methyltetrahydrofolate-ho... | XR_001742076.1 | 61.4% | 1_103del;1007_1146del;2338_3406del | |
| 16 | human | 4552 | MTRR | 5-methyltetrahydrofolate-ho... | XR_001742074.1 | 61.3% | 1_103del;1156_1157ins89;2109_3177del | |
| 17 | human | 4552 | MTRR | 5-methyltetrahydrofolate-ho... | XR_001742072.1 | 61.2% | 1_103del;1430_1581del;2350_3418del | |
| 18 | human | 4552 | MTRR | 5-methyltetrahydrofolate-ho... | XR_001742077.1 | 61% | (many diffs) | |
| 19 | human | 4552 | MTRR | 5-methyltetrahydrofolate-ho... | XR_001742071.1 | 60.8% | (many diffs) | |
| 20 | human | 4552 | MTRR | 5-methyltetrahydrofolate-ho... | NR_157169.2 | 60.2% | 1_67del;195_196ins154;2008_3065del | |
| 21 | human | 4552 | MTRR | 5-methyltetrahydrofolate-ho... | NR_157171.2 | 59.8% | (many diffs) | |
| 22 | human | 4552 | MTRR | 5-methyltetrahydrofolate-ho... | NR_134482.2 | 59.7% | 1_93del;221_222ins154;2034_3091del | |
| 23 | human | 4552 | MTRR | 5-methyltetrahydrofolate-ho... | NR_157176.2 | 58.8% | (many diffs) | |
| 24 | human | 4552 | MTRR | 5-methyltetrahydrofolate-ho... | XR_001742075.1 | 58.6% | (many diffs) | |
| 25 | human | 4552 | MTRR | 5-methyltetrahydrofolate-ho... | NR_157170.2 | 57.3% | (many diffs) | |
| 26 | human | 4552 | MTRR | 5-methyltetrahydrofolate-ho... | NR_157174.2 | 57.1% | (many diffs) | |
| 27 | human | 4552 | MTRR | 5-methyltetrahydrofolate-ho... | NR_157172.2 | 57% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2163
- ORF length:
- 2094
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gaggaggttt ctgttactat atgctacaca gcagggacag gcaaaggcca 121 tcgcagaaga aatatgtgag caagctgtgg tacatggatt ttctgcagat cttcactgta 181 ttagtgaatc cgataagtat gacctaaaaa ccgaaacagc tcctcttgtt gttgtggttt 241 ctaccacggg caccggagac ccacccgaca cagcccgcaa gtttgttaag gaaatacaga 301 accaaacact gccggttgat ttctttgctc acctgcggta tgggttactg ggtctcggtg 361 attcagaata cacctacttt tgcaatgggg ggaagataat tgataaacga cttcaagagc 421 ttggagcccg gcatttctat gacactggac atgcagatga ctgtgtaggt ttagaacttg 481 tggttgagcc gtggattgct ggactctggc cagccctcag aaagcatttt aggtcaagca 541 gaggacaaga ggagataagt ggcgcactcc cggtggcatc acctgcatcc tcgaggacag 601 accttgtgaa gtcagagctg ctacacattg aatctcaagt cgagcttctg agattcgatg 661 attcaggaag aaaggattct gaggttttga agcaaaatgc agtgaacagc aaccaatcca 721 atgttgtaat tgaagacttt gagtcctcac ttacccgttc ggtaccccca ctctcacaag 781 cctctctgaa tattcctggt ttacccccag aatatttaca ggtacatctg caggagtctc 841 ttggccagga ggaaagccaa gtatctgtga cttcagcaga tccagttttt caagtgccaa 901 tttcaaaggc agttcaactt actacgaatg atgccataaa aaccactctg ctggtagaat 961 tggacatttc aaatacagac ttttcctatc agcctggaga tgccttcagc gtgatctgcc 1021 ctaacagtga ttctgaggta caaagcctac tccaaagact gcagcttgaa gataaaagag 1081 agcactgcgt ccttttgaaa ataaaggcag acacaaagaa gaaaggagct accttacccc 1141 agcatatacc tgcgggatgt tctctccagt tcatttttac ctggtgtctt gaaatccgag 1201 caattcctaa aaaggcattt ttgcgagccc ttgtggacta taccagtgac agtgctgaaa 1261 agcgcaggct acaggagctg tgcagtaaac aaggggcagc cgattatagc cgctttgtac 1321 gagatgcctg tgcctgcttg ttggatctcc tcctcgcttt cccttcttgc cagccaccac 1381 tcagtctcct gctcgaacat cttcctaaac ttcaacccag accatattcg tgtgcaagct 1441 caagtttatt tcacccagga aagctccatt ttgtcttcaa cattgtggaa tttctgtcta 1501 ctgccacaac agaggttctg cggaagggag tatgtacagg ctggctggcc ttgttggttg 1561 cttcagttct tcagccaaac atacatgcat cccatgaaga cagcgggaaa gccctggctc 1621 ctaagatatc catctctcct cgaacaacaa attctttcca cttaccagat gacccctcaa 1681 tccccatcat aatggtgggt ccaggaaccg gcatagcccc gtttattggg ttcctacaac 1741 atagagagaa actccaagaa caacacccag atggaaattt tggagcaatg tggttgtttt 1801 ttggctgcag gcataaggat agggattatc tattcagaaa agagctcaga catttcctta 1861 agcatgggat cttaactcat ctaaaggttt ccttctcaag agatgctcct gttggggagg 1921 aggaagcccc agcaaagtat gtgcaagaca acatccagct tcatggccag caggtggcga 1981 gaatcctcct ccaggagaac ggccatattt atgtgtgtgg agatgcaaag aatatggcca 2041 aggatgtaca tgatgccctt gtgcaaataa taagcaaaga ggttggagtt gaaaaactag 2101 aagcaatgaa aaccctggcc actttaaaag aagaaaaacg ctaccttcag gatatttggt 2161 catnnccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 2221 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 2281 tctggatta