Construct: ORF ccsbBroad304_15513

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010929.1_s304c1, BRDN0000404972
Derived from:
ccsbBroadEn_15513
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NUMA1 (4926)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15513
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4926 NUMA1 nuclear mitotic apparatus p... NR_104476.2 74.7% 1_325del;3263_3928del
2 human 4926 NUMA1 nuclear mitotic apparatus p... NM_001286561.1 46.5% 46.5% 1240_4605del
3 human 4926 NUMA1 nuclear mitotic apparatus p... XM_011545066.2 46.5% 46.5% 1240_4605del
4 human 4926 NUMA1 nuclear mitotic apparatus p... XM_017017830.1 46.5% 46.5% 1240_4605del
5 human 4926 NUMA1 nuclear mitotic apparatus p... XM_017017831.1 46.5% 46.5% 1240_4605del
6 human 4926 NUMA1 nuclear mitotic apparatus p... NM_006185.3 46.2% 46.2% 1239_4646del
7 human 4926 NUMA1 nuclear mitotic apparatus p... XM_006718564.2 46.2% 46.2% 1239_4646del
8 human 4926 NUMA1 nuclear mitotic apparatus p... XM_024448556.1 46.2% 46.2% 1239_4646del
9 human 4926 NUMA1 nuclear mitotic apparatus p... XM_011545065.2 46.2% 46.2% 1240_4605del;5173_5226del
10 human 4926 NUMA1 nuclear mitotic apparatus p... XM_011545054.2 45.8% 45.8% 1239_4646del;5215_5268del
11 human 4926 NUMA1 nuclear mitotic apparatus p... XM_011545055.1 45.8% 45.8% 1239_4646del;5215_5268del
12 human 4926 NUMA1 nuclear mitotic apparatus p... XM_011545056.2 45.8% 45.8% 1239_4646del;5215_5268del
13 human 4926 NUMA1 nuclear mitotic apparatus p... XM_011545057.2 45.8% 45.8% 1239_4646del;5215_5268del
14 human 4926 NUMA1 nuclear mitotic apparatus p... XM_011545058.1 45.8% 45.8% 1239_4646del;5215_5268del
15 human 4926 NUMA1 nuclear mitotic apparatus p... XM_011545059.2 45.8% 45.8% 1239_4646del;5215_5268del
16 human 4926 NUMA1 nuclear mitotic apparatus p... XM_011545060.2 45.8% 45.8% 1239_4646del;5215_5268del
17 human 4926 NUMA1 nuclear mitotic apparatus p... XM_011545061.2 45.8% 45.8% 1239_4646del;5215_5268del
18 human 4926 NUMA1 nuclear mitotic apparatus p... XM_011545062.2 45.8% 45.8% 1239_4646del;5215_5268del
19 human 4926 NUMA1 nuclear mitotic apparatus p... XM_011545063.3 45.8% 45.8% 1239_4646del;5215_5268del
20 human 4926 NUMA1 nuclear mitotic apparatus p... XM_011545064.1 45.8% 45.8% 1239_4646del;5215_5268del
21 human 4926 NUMA1 nuclear mitotic apparatus p... XM_024448555.1 45.8% 45.8% 1239_4646del;5215_5268del
22 mouse 101706 Numa1 nuclear mitotic apparatus p... NM_133947.3 39.7% 40.2% (many diffs)
23 mouse 101706 Numa1 nuclear mitotic apparatus p... XM_006507177.2 39.7% 40.2% (many diffs)
24 mouse 101706 Numa1 nuclear mitotic apparatus p... XM_006507169.2 39.5% 39.9% (many diffs)
25 mouse 101706 Numa1 nuclear mitotic apparatus p... XM_006507170.2 39.5% 39.9% (many diffs)
26 mouse 101706 Numa1 nuclear mitotic apparatus p... XM_006507171.3 39.5% 39.9% (many diffs)
27 mouse 101706 Numa1 nuclear mitotic apparatus p... XM_006507172.3 39.5% 39.9% (many diffs)
28 mouse 101706 Numa1 nuclear mitotic apparatus p... XM_006507173.2 39.5% 39.9% (many diffs)
29 mouse 101706 Numa1 nuclear mitotic apparatus p... XM_006507174.1 39.5% 39.9% (many diffs)
30 mouse 101706 Numa1 nuclear mitotic apparatus p... XM_006507175.2 39.5% 39.9% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3003
ORF length:
2937
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgac actccacgcc acccgggggg ctgcactcct ctcttgggtg aacagtctac
121acgtggctga ccctgtggag gctgtgctgc agctccagga ctgcagcatc ttcatcaaga
181tcattgacag aatccatggc actgaagagg gacagcaaat cttgaagcag ccggtgtcag
241agagactgga ctttgtgtgc agttttctgc agaaaaatcg aaaacatccc tcttccccag
301aatgcctggt atctgcacag aaggtgctag agggatcaga gctggaactg gcgaagatga
361ccatgctgct cttataccac tctaccatga gctccaaaag tcccagggac tgggaacagt
421ttgaatataa aattcaggct gagttggctg tcattcttaa atttgtgctg gaccatgagg
481acgggctaaa ccttaatgag gacctagaga acttcctaca gaaagctcct gtgccttcta
541cctgttctag cacattccct gaagagctct ccccacctag ccaccaggcc aagagggaga
601ttcgcttcct agagctacag aaggttgcct cctcttccag tgggaacaac tttctctcag
661gttctccagc ttctcccatg ggtgatatcc tgcagacccc acagttccag atgagacggc
721tgaagaagca gcttgctgat gagagaagta atagggatga gctggagctg gagctagctg
781agaaccgcaa gctcctcacc gagaaggatg cacagatagc catgatgcag cagcgcattg
841accgcctagc cctgctgaat gagaagcagg cggccagccc actggagccc aaggagcttg
901aggagctgcg tgacaagaat gagagcctta ccatgcggct gcatgaaacc ctgaagcagt
961gccaggacct gaagacagag aagagccaga tggatcgcaa aatcaaccag ctttcggagg
1021agaatggaga cctttccttt aagctgcggg agtttgccag tcatctgcag cagctacagg
1081atgccctcaa tgagctgacg gaggagcaca gcaaggccac tcaggagtgg ctagagaagc
1141aggcccagct ggagaaggag ctcagcgcag ccctgcagga caagaaatgc cttgaagaga
1201agaacgaaat ccttcaggga aaactttcac agctggaaga acacttgtcc cagctgcagg
1261ataacccacc ccaggagaag ggcgaggtgc tgggtgatgt cttgcaggtg gaagaactga
1321gtaagaaact ggctgactct gaccaagcca gcaaggtgca gcagcagaag ctgaaggctg
1381tccaggctca gggaggcgag agccagcagg aggcccagcg cctccaggcc cagctgaatg
1441aactgcaagc ccagttgagc cagaaggagc aggcagctga gcactataag ctgcagatgg
1501agaaagccaa aacacattat gatgccaaga agcagcagaa ccaagagctg caggagcagc
1561tgcggagcct ggagcagctg cagaaggaaa acaaagagct gcgagctgaa gctgaacggc
1621tgggccatga gctacagcag gctgggctga agaccaagga ggctgaacag acctgccgcc
1681accttactgc ccaggtgcgc agcctggagg cacaggttgc ccatgcagac cagcagcttc
1741gagacctggg caaattccag gtggcaactg atgctttaaa gagccgtgag ccccaggcta
1801agccccagct ggacttgagt attgacagcc tggatctgag ctgcgaggag gggaccccac
1861tcagtatcac cagcaagctg cctcgtaccc agccagacgg caccagcgtc cctggagaac
1921cagcctcacc tatctcccag cgcctgcccc ccaaggtaga atccctggag agtctctact
1981tcactcccat ccctgctcgg agtcaggccc ccctggagag cagcctggac tccctgggag
2041acgtcttcct ggactcgggt cgtaagaccc gctccgctcg tcggcgcacc acgcagatca
2101tcaacatcac catgaccaag aagctagatg tggaagagcc agacagcgcc aactcatcgt
2161tctacagcac gcggtctgct cctgcttccc aggctagcct gcgagccacc tcctctactc
2221agtctctagc tcgcctgggt tctcccgatt atggcaactc agccctgctc agcttgcctg
2281gctaccgccc caccactcgc agttctgctc gtcgttccca ggccggggtg tccagtgggg
2341cccctccagg aaggaacagc ttctacatgg gcacttgcca ggatgagcct gagcagctgg
2401atgactggaa ccgcattgca gagctgcagc agcgcaatcg agtgtgcccc ccacatctga
2461agacctgcta tcccctggag tccaggcctt ccctgagcct gggcaccatc acagatgagg
2521agatgaaaac tggagacccc caagagaccc tgcgccgagc cagcatgcag ccaatccaga
2581tagccgaggg cactggcatc accacccggc agcagcgcaa acgggtctcc ctagagcccc
2641accagggccc tggaactcct gagtctaaga aggccaccag ctgtttccca cgccccatga
2701ctccccgaga ccgacatgaa gggcgcaaac agagcactac tgaggcccag aagaaagcag
2761ctccagcttc tactaaacag gctgaccggc gccagtcgat ggccttcagc atcctcaaca
2821cacccaagaa gctagggaac agccttctgc ggcggggagc ctcaaagaag gccctgtcca
2881aggcttcccc caacactcgc agtggaaccc gccgttctcc gcgcattgcc accaccacag
2941ccagcgccgc cactgctgcc gccattggtg ccacccctcg agccaagggc aaggcaaagc
3001actnnccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc
3061tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc
3121tctggatta

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