Construct: ORF ccsbBroad304_15513
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010929.1_s304c1, BRDN0000404972
- Derived from:
- ccsbBroadEn_15513
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NUMA1 (4926)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15513
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4926 | NUMA1 | nuclear mitotic apparatus p... | NR_104476.2 | 74.7% | 1_325del;3263_3928del | |
| 2 | human | 4926 | NUMA1 | nuclear mitotic apparatus p... | NM_001286561.1 | 46.5% | 46.5% | 1240_4605del |
| 3 | human | 4926 | NUMA1 | nuclear mitotic apparatus p... | XM_011545066.2 | 46.5% | 46.5% | 1240_4605del |
| 4 | human | 4926 | NUMA1 | nuclear mitotic apparatus p... | XM_017017830.1 | 46.5% | 46.5% | 1240_4605del |
| 5 | human | 4926 | NUMA1 | nuclear mitotic apparatus p... | XM_017017831.1 | 46.5% | 46.5% | 1240_4605del |
| 6 | human | 4926 | NUMA1 | nuclear mitotic apparatus p... | NM_006185.3 | 46.2% | 46.2% | 1239_4646del |
| 7 | human | 4926 | NUMA1 | nuclear mitotic apparatus p... | XM_006718564.2 | 46.2% | 46.2% | 1239_4646del |
| 8 | human | 4926 | NUMA1 | nuclear mitotic apparatus p... | XM_024448556.1 | 46.2% | 46.2% | 1239_4646del |
| 9 | human | 4926 | NUMA1 | nuclear mitotic apparatus p... | XM_011545065.2 | 46.2% | 46.2% | 1240_4605del;5173_5226del |
| 10 | human | 4926 | NUMA1 | nuclear mitotic apparatus p... | XM_011545054.2 | 45.8% | 45.8% | 1239_4646del;5215_5268del |
| 11 | human | 4926 | NUMA1 | nuclear mitotic apparatus p... | XM_011545055.1 | 45.8% | 45.8% | 1239_4646del;5215_5268del |
| 12 | human | 4926 | NUMA1 | nuclear mitotic apparatus p... | XM_011545056.2 | 45.8% | 45.8% | 1239_4646del;5215_5268del |
| 13 | human | 4926 | NUMA1 | nuclear mitotic apparatus p... | XM_011545057.2 | 45.8% | 45.8% | 1239_4646del;5215_5268del |
| 14 | human | 4926 | NUMA1 | nuclear mitotic apparatus p... | XM_011545058.1 | 45.8% | 45.8% | 1239_4646del;5215_5268del |
| 15 | human | 4926 | NUMA1 | nuclear mitotic apparatus p... | XM_011545059.2 | 45.8% | 45.8% | 1239_4646del;5215_5268del |
| 16 | human | 4926 | NUMA1 | nuclear mitotic apparatus p... | XM_011545060.2 | 45.8% | 45.8% | 1239_4646del;5215_5268del |
| 17 | human | 4926 | NUMA1 | nuclear mitotic apparatus p... | XM_011545061.2 | 45.8% | 45.8% | 1239_4646del;5215_5268del |
| 18 | human | 4926 | NUMA1 | nuclear mitotic apparatus p... | XM_011545062.2 | 45.8% | 45.8% | 1239_4646del;5215_5268del |
| 19 | human | 4926 | NUMA1 | nuclear mitotic apparatus p... | XM_011545063.3 | 45.8% | 45.8% | 1239_4646del;5215_5268del |
| 20 | human | 4926 | NUMA1 | nuclear mitotic apparatus p... | XM_011545064.1 | 45.8% | 45.8% | 1239_4646del;5215_5268del |
| 21 | human | 4926 | NUMA1 | nuclear mitotic apparatus p... | XM_024448555.1 | 45.8% | 45.8% | 1239_4646del;5215_5268del |
| 22 | mouse | 101706 | Numa1 | nuclear mitotic apparatus p... | NM_133947.3 | 39.7% | 40.2% | (many diffs) |
| 23 | mouse | 101706 | Numa1 | nuclear mitotic apparatus p... | XM_006507177.2 | 39.7% | 40.2% | (many diffs) |
| 24 | mouse | 101706 | Numa1 | nuclear mitotic apparatus p... | XM_006507169.2 | 39.5% | 39.9% | (many diffs) |
| 25 | mouse | 101706 | Numa1 | nuclear mitotic apparatus p... | XM_006507170.2 | 39.5% | 39.9% | (many diffs) |
| 26 | mouse | 101706 | Numa1 | nuclear mitotic apparatus p... | XM_006507171.3 | 39.5% | 39.9% | (many diffs) |
| 27 | mouse | 101706 | Numa1 | nuclear mitotic apparatus p... | XM_006507172.3 | 39.5% | 39.9% | (many diffs) |
| 28 | mouse | 101706 | Numa1 | nuclear mitotic apparatus p... | XM_006507173.2 | 39.5% | 39.9% | (many diffs) |
| 29 | mouse | 101706 | Numa1 | nuclear mitotic apparatus p... | XM_006507174.1 | 39.5% | 39.9% | (many diffs) |
| 30 | mouse | 101706 | Numa1 | nuclear mitotic apparatus p... | XM_006507175.2 | 39.5% | 39.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3003
- ORF length:
- 2937
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgac actccacgcc acccgggggg ctgcactcct ctcttgggtg aacagtctac 121 acgtggctga ccctgtggag gctgtgctgc agctccagga ctgcagcatc ttcatcaaga 181 tcattgacag aatccatggc actgaagagg gacagcaaat cttgaagcag ccggtgtcag 241 agagactgga ctttgtgtgc agttttctgc agaaaaatcg aaaacatccc tcttccccag 301 aatgcctggt atctgcacag aaggtgctag agggatcaga gctggaactg gcgaagatga 361 ccatgctgct cttataccac tctaccatga gctccaaaag tcccagggac tgggaacagt 421 ttgaatataa aattcaggct gagttggctg tcattcttaa atttgtgctg gaccatgagg 481 acgggctaaa ccttaatgag gacctagaga acttcctaca gaaagctcct gtgccttcta 541 cctgttctag cacattccct gaagagctct ccccacctag ccaccaggcc aagagggaga 601 ttcgcttcct agagctacag aaggttgcct cctcttccag tgggaacaac tttctctcag 661 gttctccagc ttctcccatg ggtgatatcc tgcagacccc acagttccag atgagacggc 721 tgaagaagca gcttgctgat gagagaagta atagggatga gctggagctg gagctagctg 781 agaaccgcaa gctcctcacc gagaaggatg cacagatagc catgatgcag cagcgcattg 841 accgcctagc cctgctgaat gagaagcagg cggccagccc actggagccc aaggagcttg 901 aggagctgcg tgacaagaat gagagcctta ccatgcggct gcatgaaacc ctgaagcagt 961 gccaggacct gaagacagag aagagccaga tggatcgcaa aatcaaccag ctttcggagg 1021 agaatggaga cctttccttt aagctgcggg agtttgccag tcatctgcag cagctacagg 1081 atgccctcaa tgagctgacg gaggagcaca gcaaggccac tcaggagtgg ctagagaagc 1141 aggcccagct ggagaaggag ctcagcgcag ccctgcagga caagaaatgc cttgaagaga 1201 agaacgaaat ccttcaggga aaactttcac agctggaaga acacttgtcc cagctgcagg 1261 ataacccacc ccaggagaag ggcgaggtgc tgggtgatgt cttgcaggtg gaagaactga 1321 gtaagaaact ggctgactct gaccaagcca gcaaggtgca gcagcagaag ctgaaggctg 1381 tccaggctca gggaggcgag agccagcagg aggcccagcg cctccaggcc cagctgaatg 1441 aactgcaagc ccagttgagc cagaaggagc aggcagctga gcactataag ctgcagatgg 1501 agaaagccaa aacacattat gatgccaaga agcagcagaa ccaagagctg caggagcagc 1561 tgcggagcct ggagcagctg cagaaggaaa acaaagagct gcgagctgaa gctgaacggc 1621 tgggccatga gctacagcag gctgggctga agaccaagga ggctgaacag acctgccgcc 1681 accttactgc ccaggtgcgc agcctggagg cacaggttgc ccatgcagac cagcagcttc 1741 gagacctggg caaattccag gtggcaactg atgctttaaa gagccgtgag ccccaggcta 1801 agccccagct ggacttgagt attgacagcc tggatctgag ctgcgaggag gggaccccac 1861 tcagtatcac cagcaagctg cctcgtaccc agccagacgg caccagcgtc cctggagaac 1921 cagcctcacc tatctcccag cgcctgcccc ccaaggtaga atccctggag agtctctact 1981 tcactcccat ccctgctcgg agtcaggccc ccctggagag cagcctggac tccctgggag 2041 acgtcttcct ggactcgggt cgtaagaccc gctccgctcg tcggcgcacc acgcagatca 2101 tcaacatcac catgaccaag aagctagatg tggaagagcc agacagcgcc aactcatcgt 2161 tctacagcac gcggtctgct cctgcttccc aggctagcct gcgagccacc tcctctactc 2221 agtctctagc tcgcctgggt tctcccgatt atggcaactc agccctgctc agcttgcctg 2281 gctaccgccc caccactcgc agttctgctc gtcgttccca ggccggggtg tccagtgggg 2341 cccctccagg aaggaacagc ttctacatgg gcacttgcca ggatgagcct gagcagctgg 2401 atgactggaa ccgcattgca gagctgcagc agcgcaatcg agtgtgcccc ccacatctga 2461 agacctgcta tcccctggag tccaggcctt ccctgagcct gggcaccatc acagatgagg 2521 agatgaaaac tggagacccc caagagaccc tgcgccgagc cagcatgcag ccaatccaga 2581 tagccgaggg cactggcatc accacccggc agcagcgcaa acgggtctcc ctagagcccc 2641 accagggccc tggaactcct gagtctaaga aggccaccag ctgtttccca cgccccatga 2701 ctccccgaga ccgacatgaa gggcgcaaac agagcactac tgaggcccag aagaaagcag 2761 ctccagcttc tactaaacag gctgaccggc gccagtcgat ggccttcagc atcctcaaca 2821 cacccaagaa gctagggaac agccttctgc ggcggggagc ctcaaagaag gccctgtcca 2881 aggcttcccc caacactcgc agtggaaccc gccgttctcc gcgcattgcc accaccacag 2941 ccagcgccgc cactgctgcc gccattggtg ccacccctcg agccaagggc aaggcaaagc 3001 actnnccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 3061 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 3121 tctggatta