Construct: ORF ccsbBroad304_15521
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002564.1_s304c1, BRDN0000403121
- Derived from:
- ccsbBroadEn_15521
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SERPINF1 (5176)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15521
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5176 | SERPINF1 | serpin family F member 1 | NM_001329903.1 | 99.7% | 99.5% | 215C>T;395C>G;963T>C |
| 2 | human | 5176 | SERPINF1 | serpin family F member 1 | NM_002615.7 | 99.7% | 99.5% | 215C>T;395C>G;963T>C |
| 3 | human | 5176 | SERPINF1 | serpin family F member 1 | NM_001329904.1 | 55.1% | 55.2% | 0_1ins561;402T>C |
| 4 | human | 5176 | SERPINF1 | serpin family F member 1 | NM_001329905.1 | 55.1% | 55.2% | 0_1ins561;402T>C |
| 5 | mouse | 20317 | Serpinf1 | serine (or cysteine) peptid... | NM_011340.3 | 83.7% | 85.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1320
- ORF length:
- 1254
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgca ggccctggtg ctactcctct gcattggagc cctcctcggg cacagcagct 121 gccagaaccc tgccagcccc ccggaggagg gctccccaga ccccgacagc acaggggcgc 181 tggtggagga ggaggatcct ttcttcaaag tccccgtgaa caagctggca gcggctgtct 241 ccaacttcgg ctatgacctg taccgggtgc gatccagcat gagccccacg accaacgtgc 301 tcctgtctcc tctcagtgtg gccacggccc tctcggccct ctcgctggga gcggagcagc 361 gaacagaatc catcattcac cgggctctct actatgactt gatcagcagc ccagacatcc 421 atggtaccta taaggagctc cttgacacgg tcactgcccg ccagaagaac ctcaagagtg 481 cctcccggat cgtctttgag aagaagctgc gcataaaatc cagctttgtg gcacctctgg 541 aaaagtcata tgggaccagg cccagagtcc tgacgggcaa ccctcgcttg gacctgcaag 601 agatcaacaa ctgggtgcag gcgcagatga aagggaagct cgccaggtcc acaaaggaaa 661 ttcccgatga gatcagcatt ctccttctcg gtgtggcgca cttcaagggg cagtgggtaa 721 caaagtttga ctccagaaag acttccctcg aggatttcta cttggatgaa gagaggaccg 781 tgagggtccc catgatgtcg gaccctaagg ctgttttacg ctatggcttg gattcagatc 841 tcagctgcaa gattgcccag ctgcccttga ccggaagcat gagtatcatc ttcttcctgc 901 ccctgaaagt gacccagaat ttgaccttga tagaggagag cctcacctcc gagttcattc 961 atgacataga ccgagaactg aagaccgtgc aggcggtcct cactgtcccc aagctgaagc 1021 tgagttacga aggcgaagtc accaagtccc tgcaggagat gaagctgcaa tccttgtttg 1081 attcaccaga ctttagcaag atcacaggca aacccatcaa gctgactcag gtggaacacc 1141 gggctggctt tgagtggaac gaggatgggg cgggaaccac ccccagccca gggctgcagc 1201 ctgcccacct caccttcccg ctggactatc accttaacca gcctttcatc ttcgtactga 1261 gggacacaga cacaggggcc cttctcttca ttggcaagat tctggacccc aggggcccct 1321 nnccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 1381 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 1441 ggatta