Construct: ORF ccsbBroad304_15525
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003044.1_s304c1, BRDN0000411452
- Derived from:
- ccsbBroadEn_15525
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PFKL (5211)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15525
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5211 | PFKL | phosphofructokinase, liver ... | XM_011529603.2 | 83.3% | 83.3% | 1_279del |
| 2 | human | 5211 | PFKL | phosphofructokinase, liver ... | XM_005261137.4 | 61.9% | 61.9% | 1_858del |
| 3 | human | 5211 | PFKL | phosphofructokinase, liver ... | XM_017028370.1 | 61.9% | 61.9% | 1_858del |
| 4 | human | 5211 | PFKL | phosphofructokinase, liver ... | XM_017028372.1 | 61.9% | 61.9% | 1_858del |
| 5 | human | 5211 | PFKL | phosphofructokinase, liver ... | XM_017028369.1 | 61.4% | 61.4% | 1_876del |
| 6 | human | 5211 | PFKL | phosphofructokinase, liver ... | NM_002626.6 | 59.6% | 59.6% | 1_945del |
| 7 | human | 5211 | PFKL | phosphofructokinase, liver ... | XM_005261135.3 | 57.9% | 57.9% | 1_1014del |
| 8 | human | 5211 | PFKL | phosphofructokinase, liver ... | NM_001002021.3 | 56% | 56% | 1_1095del |
| 9 | human | 5211 | PFKL | phosphofructokinase, liver ... | XM_024452085.1 | 56% | 56% | 1_1095del |
| 10 | human | 5211 | PFKL | phosphofructokinase, liver ... | XM_006724011.1 | 54.5% | 54.5% | 1_1164del |
| 11 | human | 5211 | PFKL | phosphofructokinase, liver ... | XM_017028368.1 | 54.5% | 54.5% | 1_1164del |
| 12 | mouse | 18641 | Pfkl | phosphofructokinase, liver,... | XM_017313846.1 | 87.5% | 93.1% | (many diffs) |
| 13 | mouse | 18641 | Pfkl | phosphofructokinase, liver,... | NM_008826.4 | 52.2% | 55.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1461
- ORF length:
- 1395
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg catggaggcg gtgatggcgc tgctggaagc cacgcctgac acgccggcct 121 gcgtggtcac cctctcgggg aaccagtcag tgcggctgcc cctcatggag tgcgtgcaga 181 tgaccaagga agtgcagaaa gccatggatg acaagaggtt tgacgaggcc acccagctcc 241 gtggtgggag cttcgagaac aactggaaca tttacaagct cctcgcccac cagaagcccc 301 ccaaggagaa gtctaacttc tccctggcca tcctgaatgt gggggccccg gcggctggca 361 tgaatgcggc cgtgcgctcg gcggtgcgga ccggcatctc ccatggacac acagtatacg 421 tggtgcacga tggcttcgaa ggcctagcca agggtcaggt gcaagaagta ggctggcacg 481 acgtggccgg ctggttgggg cgtggtggct ccatgctggg gaccaagagg accctgccca 541 agggccagct ggagtccatt gtggagaaca tccgcatcta tggtattcac gccctgctgg 601 tggtcggtgg gtttgaggcc tatgaagggg tgctgcagct ggtggaggct cgcgggcgct 661 acgaggagct ctgcatcgtc atgtgtgtca tcccagccac catcagcaac aacgtccctg 721 gcaccgactt cagcctgggc tccgacactg ctgtaaatgc cgccatggag agctgtgacc 781 gcatcaaaca gtctgcctcg gggaccaagc gccgtgtgtt catcgtggag accatggggg 841 gttactgtgg ctacctggcc accgtgactg gcattgctgt gggggccgac gccgcctacg 901 tcttcgagga ccctttcaac atccacgact taaaggtcaa cgtggagcac atgacggaga 961 agatgaagac agacattcag aggggcctgg tgctgcggaa cgagaagtgc catgactact 1021 acaccacgga gttcctgtac aacctgtact catcagaggg caagggcgtc ttcgactgca 1081 ggaccaatgt cctgggccac ctgcagcagg gtggcgctcc aacccccttt gaccggaact 1141 atgggaccaa gctgggggtg aaggccatgc tgtggttgtc ggagaagctg cgcgaggttt 1201 accgcaaggg acgggtgttc gccaatgccc cagactcggc ctgcgtgatc ggcctgaaga 1261 agaaggcggt ggccttcagc cccgtcactg agctcaagaa agacactgat ttcgagcacc 1321 gcatgccacg ggagcagtgg tggctgagcc tgcggctcat gctgaagatg ctggcacaat 1381 accgcatcag tatggccgcc tacgtgtcag gggagctgga gcacgtgacc cgccgcaccc 1441 tgagcatgga caagggcttc tnnccaactt tcttgtacaa agtggttggt aagcctatcc 1501 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 1561 ttaagtcgac aatcaacctc tggatta