Construct: ORF ccsbBroad304_15574
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015310.1_s304c1, BRDN0000410865
- Derived from:
- ccsbBroadEn_15574
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPL37A (6168)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15574
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6168 | RPL37A | ribosomal protein L37a | NM_000998.5 | 77.8% | 77.1% | 216_276delinsG |
| 2 | mouse | 19981 | Rpl37a | ribosomal protein L37a | NM_009084.4 | 71.7% | 77.1% | (many diffs) |
| 3 | mouse | 70873 | Cnbd2 | cyclic nucleotide binding d... | XM_017319256.1 | 32.9% | 34.5% | (many diffs) |
| 4 | mouse | 751556 | Mir682 | microRNA 682 | NR_030451.1 | 23.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 282
- ORF length:
- 216
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc caaacgtacc aagaaagtcg ggatcgtcgg taaatacggg acccgctatg 121 gggcctccct ccggaaaatg gtgaagaaaa ttgaaatcag ccagcacgcc aagtacactt 181 gctctttctg tggcaaaacc aagatgaaga gacgagctgt ggggatctgg cactgtggtt 241 cctgcatgaa gacagtggct ggcggtgcct ggacgtacaa gtnnccaact ttcttgtaca 301 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 361 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta