Construct: ORF ccsbBroad304_15604
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014764.1_s304c1, BRDN0000409011
- Derived from:
- ccsbBroadEn_15604
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TARS1 (6897)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15604
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6897 | TARS1 | threonyl-tRNA synthetase 1 | NM_001258437.1 | 98.2% | 98.2% | 1_36del;1358C>T |
| 2 | human | 6897 | TARS1 | threonyl-tRNA synthetase 1 | NM_152295.4 | 98.2% | 98.2% | 1_36del;1358C>T |
| 3 | human | 6897 | TARS1 | threonyl-tRNA synthetase 1 | NM_001258438.1 | 94% | 93.9% | 1_36del;330_428del;1457C>T |
| 4 | human | 6897 | TARS1 | threonyl-tRNA synthetase 1 | NR_047676.1 | 71.1% | (many diffs) | |
| 5 | human | 6897 | TARS1 | threonyl-tRNA synthetase 1 | NR_047677.1 | 68.5% | (many diffs) | |
| 6 | human | 6897 | TARS1 | threonyl-tRNA synthetase 1 | NR_047678.1 | 67.5% | (many diffs) | |
| 7 | mouse | 110960 | Tars | threonyl-tRNA synthetase | NM_033074.3 | 85.5% | 93.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2199
- ORF length:
- 2133
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg aggcgaggag aagccgattg gtgctggtga agagaagcaa aaggaaggag 121 gcaaaaagaa gaacaaagaa ggatctggag atggaggtcg agctgagttg aatccttggc 181 ctgaatatat ttacacacgt cttgagatgt ataatatact aaaagcagaa catgattcca 241 ttctggcaga aaaggcagaa aaagatagca agccaattaa agtcactttg cctgatggta 301 aacaggttga tgcggaatct tggaaaacta caccatatca aattgcctgt ggaattagtc 361 aaggcctggc cgacaacacc gttattgcta aagtaaataa tgttgtgtgg gacctggacc 421 gccctctgga agaagattgt accttggagc ttctcaagtt tgaggatgag gaagctcagg 481 cagtgtattg gcactctagt gctcacataa tgggtgaagc catggaaaga gtctatggtg 541 gatgtttatg ctacggtccg ccaatagaaa atggattcta ttatgacatg tacctcgaag 601 aagggggtgt gtctagcaat gatttctctt ctctggaggc tttgtgtaag aaaatcatta 661 aagaaaaaca agcttttgaa agactggaag ttaagaaaga aactttactg gcaatgttta 721 agtacaacaa gttcaaatgc cggatattga atgaaaaggt gaatactcca actaccacag 781 tctatagatg tggccctttg atagatctct gccggggtcc tcatgttaga cacacgggca 841 aaattaaggc tttaaaaata cacaaaaatt cctccacgta ctgggaaggc aaagcagata 901 tggagactct ccagagaatt tatggcattt cattcccaga tcctaaaatg ttgaaagagt 961 gggagaagtt ccaagaggaa gctaaaaacc gagatcatag gaaaattggc agggaccaag 1021 aactatattt ctttcatgaa ctcagccctg gaagttgctt ttttctgcca aaaggagcct 1081 acatttataa tgcacttatt gaattcatta ggagcgaata taggaaaaga ggattccagg 1141 aggtagtcac cccaaacatc ttcaacagcc gactctggat gacctcgggc cactggcagc 1201 actacagcga gaacatgttc tcctttgagg tggagaagga gctgtttgcc ctgaaaccca 1261 tgaactgccc aggacactgc cttatgtttg atcatcggcc aaggtcctgg cgagaactgc 1321 ctctgcggct agctgatttt ggggtacttc ataggaacga gctgtctgga gcactcacag 1381 gactcatccg ggtacgaaga ttccaacagg atgatgctca catattctgt gccatggagc 1441 agattgaaga tgaaataaaa ggttgtttgg attttctacg tacggtatat agcgtatttg 1501 gattttcttt taaactaaac ctttctactc gcccggaaaa attccttgga gatatcgaag 1561 tatgggatca agctgagaaa caacttgaaa acagtctgaa tgaatttggt gaaaagtggg 1621 agttaaactc tggagatgga gctttctatg gcccaaagat tgacatacag attaaagatg 1681 cgattgggcg gtaccaccag tgtgcaacca tccagctgga tttccagttg cccatcagat 1741 ttaatcttac ttatgtaagc catgatggtg atgataagaa aaggccagtg attgttcatc 1801 gagccatctt gggatcagtg gaaagaatga ttgctatcct cacagaaaac tatgggggca 1861 aatggccctt ttggctgtcc cctcgccagg taatggtagt tccagtggga ccaacctgtg 1921 atgaatatgc ccaaaaggta cgacaacaat tccacgatgc caaattcatg gcagacattg 1981 atctggatcc aggctgtaca ttgaataaaa agattcgaaa tgcacagtta gcacagtata 2041 acttcatttt agttgttggt gaaaaagaga aaatcagtgg cactgttaat atccgcacaa 2101 gagacaataa ggtccacggg gaacgcacca tttctgaaac tatcgagcgg ctacagcagc 2161 tcaaagagtt ccgcagcaaa caggcagaag aagaattttn nccaactttc ttgtacaaag 2221 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 2281 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta