Construct: ORF ccsbBroad304_15618

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015703.1_s304c1, BRDN0000401838
Derived from:
ccsbBroadEn_15618
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
USP4 (7375)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15618
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7375 USP4 ubiquitin specific peptidase 4 NM_003363.4 99.9% 99.8% 1379T>G
2 human 7375 USP4 ubiquitin specific peptidase 4 NM_199443.3 95% 94.9% 695_696ins141;1238T>G
3 human 7375 USP4 ubiquitin specific peptidase 4 NM_001251877.2 29.9% 26.1% (many diffs)
4 mouse 22258 Usp4 ubiquitin specific peptidas... NM_011678.2 87.8% 90.6% (many diffs)
5 mouse 22258 Usp4 ubiquitin specific peptidas... XM_006511696.2 87.3% 90% (many diffs)
6 mouse 22258 Usp4 ubiquitin specific peptidas... NM_001311157.1 83.2% 86.2% (many diffs)
7 mouse 22258 Usp4 ubiquitin specific peptidas... XM_006511697.2 82.8% 85.7% (many diffs)
8 mouse 22258 Usp4 ubiquitin specific peptidas... XM_006511700.3 79.2% 80.9% (many diffs)
9 mouse 22258 Usp4 ubiquitin specific peptidas... XM_006511699.4 78.8% 80.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2958
ORF length:
2889
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggcggaaggt ggaggctgcc gtgagcgacc ggatgcggag actcagaagt
121ccgagcttgg acccttaatg aggaccacac tccaacgcgg ggcgcagtgg tatcttattg
181acagccggtg gttcaagcag tggaagaagt atgtgggctt tgacagctgg gacatgtaca
241atgtgggtga acataaccta tttcctggcc caatagacaa ctctgggcta ttttcagatc
301ctgagagtca gaccttgaaa gaacacttaa ttgatgaatt ggactatgta ttggtcccta
361ccgaggcgtg gaataaacta ctaaactggt acggctgtgt agaaggccag caacccatcg
421tcagaaaagt tgtggagcat ggcctgtttg tcaagcactg caaagtcgag gtgtatttgc
481tggaactgaa gctctgtgag aacagtgacc ccaccaatgt gctgagttgc catttcagca
541aggcagacac cattgcaacc atcgagaaag agatgcggaa gctattcaac atccctgcgg
601agcgtgaaac acggctctgg aacaaataca tgagcaacac ctacgagcag ttgagcaagc
661tagacaacac tgtccaggat gctgggctat accagggtca ggtgctagta attgagcctc
721aaaatgaaga tggcacatgg cccaggcaga ccttgcagtc aaaatcaagc actgcgccta
781gcagaaattt tactacctct ccaaaatcat cagcaagtcc ctattcctca gtgtctgcct
841ctctcattgc aaatggtgat agcactagca cctgtgggat gcacagttcc ggtgtcagca
901ggggtggatc tggcttttct gcttcgtata attgtcagga gccaccatcc tctcatatac
961aacctgggct ctgtggactt ggaaacctgg gaaacacctg cttcatgaac tccgctttgc
1021agtgtttgag caacactgca ccactgactg actactttct caaagatgag tatgaagccg
1081aaatcaacag agacaaccct ctggggatga aaggggaaat tgcagaagcc tatgctgaac
1141tcattaagca gatgtggtct ggaagggacg cccatgtggc acctcgcatg ttcaaaactc
1201aagtaggacg ttttgctcct caattttctg gctaccagca acaagattct caggagctgc
1261tggcctttct tctagatgga ttgcatgaag atctgaaccg ggtaaagaaa aagccctact
1321tggagctgaa ggatgccaat gggcggccag atgcggtggt ggcaaaggaa gcctgggaga
1381atcacaggtt gaggaatgat tctgtgattg tggatacttt ccatggcctc ttcaaatcta
1441ctttgggttg cccagaatgt gctaaggttt ctgtgacctt tgacccattt tgctatctaa
1501cgctgccact gcccttgaag aaagatcgag ttatggaggt tttcctggtt cctgctgacc
1561ctcactgcag acctactcag taccgtgtga ctgtgccgct gatgggggct gtgtccgacc
1621tgtgcgaggc tctctccagg ctgtctggca ttgctgcaga aaatatggtg gtcgcagatg
1681tgtataatca ccgattccac aaaattttcc aaatggatga aggtttaaac cacatcatgc
1741ctcgggatga cattttcgtg tacgaggtct gcagcacttc cgtggatggc tcggaatgtg
1801tcacgcttcc agtctacttc agggagagga agtccaggcc atcaagcact tcctccgcat
1861cagcgctata tgggcagcca ctattgcttt ctgtccccaa gcacaagtta acccttgagt
1921ctttgtacca ggctgtttgt gatcgtatca gccgctatgt gaaacagcct ttacctgatg
1981agtttggcag ctcacccttg gagccagggg cctgcaatgg ctccaggaac agctgtgaag
2041gagaagatga ggaagaaatg gagcatcagg aagaaggcaa agagcagctt tcagaaacag
2101aaggcagtgg ggaagatgag ccaggaaatg accccagtga gaccacccaa aagaagatca
2161aaggccagcc ctgcccaaaa aggcttttta ccttcagtct tgtgaactcc tatggaacag
2221ctgacataaa ttcacttgca gctgatggaa aactacttaa actcaactct cgatctacac
2281tggccatgga ttgggacagt gaaactcgga gactttacta tgatgagcaa gaatctgagg
2341cctacgagaa gcatgtgagc atgttgcagc ctcagaagaa gaagaagacc acagtggccc
2401tgagagactg catcgagctc ttcaccacca tggagaccct tggggagcat gacccctggt
2461actgtcccaa ctgtaagaag catcaacagg ccacaaaaaa gtttgaccta tggtccttgc
2521ccaagatcct ggtggtccac ctcaaacgtt tctcctacaa cagatactgg agggataagc
2581tcgacacagt cgtagaattc ccaatcagag ggctgaacat gtccgagttt gtctgtaacc
2641tgtcagcaag gccttatgtg tacgacctca ttgccgtgtc caatcattat ggagccatgg
2701gggttggcca ctacactgca tatgcgaaga acaaactgaa tggtaaatgg tattactttg
2761atgatagcaa cgtgtccctg gcctctgagg atcagatagt gactaaagca gcttatgtgc
2821tattttacca acgtcgagat gatgaatttt ataagacacc ttcacttagc agttctggtt
2881cctctgatgg agggacacga ccaagcagct ctcagcaggg ctttggggat gatgaggctt
2941gcagcatgga caccaactnn ccaactttct tgtacaaagt ggttggtaag cctatcccta
3001accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta
3061agtcgacaat caacctctgg atta

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