Construct: ORF ccsbBroad304_15670
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001779.1_s304c1, BRDN0000407542
- Derived from:
- ccsbBroadEn_15670
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BAG3 (9531)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15670
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9531 | BAG3 | BAG cochaperone 3 | NM_004281.3 | 99.9% | 100% | 1296A>G |
| 2 | human | 9531 | BAG3 | BAG cochaperone 3 | XM_005270287.2 | 99.7% | 99.8% | 906_907insCAG;1293A>G |
| 3 | mouse | 29810 | Bag3 | BCL2-associated athanogene 3 | NM_013863.5 | 84.2% | 84.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1791
- ORF length:
- 1725
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag cgccgccacc cactcgccca tgatgcaggt ggcgtccggc aacggtgacc 121 gcgacccttt gccccccgga tgggagatca agatcgaccc gcagaccggc tggcccttct 181 tcgtggacca caacagccgc accactacgt ggaacgaccc gcgcgtgccc tctgagggcc 241 ccaaggagac tccatcctct gccaatggcc cttcccggga gggctctagg ctgccgcctg 301 ctagggaagg ccaccctgtg tacccccagc tccgaccagg ctacattccc attcctgtgc 361 tccatgaagg cgctgagaac cggcaggtgc accctttcca tgtctatccc cagcctggga 421 tgcagcgatt ccgaactgag gcggcagcag cggctcctca gaggtcccag tcacctctgc 481 ggggcatgcc agaaaccact cagccagata aacagtgtgg acaggtggca gcggcggcgg 541 cagcccagcc cccagcctcc cacggacctg agcggtccca gtctccagct gcctctgact 601 gctcatcctc atcctcctcg gccagcctgc cttcctccgg caggagcagc ctgggcagtc 661 accagctccc gcgggggtac atctccattc cggtgataca cgagcagaac gttacccggc 721 cagcagccca gccctccttc caccaagccc agaagacgca ctacccagcg cagcaggggg 781 agtaccagac ccaccagcct gtgtaccaca agatccaggg ggatgactgg gagccccggc 841 ccctgcgggc ggcatccccg ttcaggtcat ctgtccaggg tgcatcgagc cgggagggct 901 caccagccag gagcagcacg ccactccact ccccctcgcc catccgtgtg cacaccgtgg 961 tcgacaggcc tcagcagccc atgacccatc gagaaactgc acctgtttcc cagcctgaaa 1021 acaaaccaga aagtaagcca ggcccagttg gaccagaact ccctcctgga cacatcccaa 1081 ttcaagtgat ccgcaaagag gtggattcta aacctgtttc ccagaagccc ccacctccct 1141 ctgagaaggt agaggtgaaa gttccccctg ctccagttcc ttgtcctcct cccagccctg 1201 gcccttctgc tgtcccctct tcccccaaga gtgtggctac agaagagagg gcagccccca 1261 gcactgcccc tgcagaagct acacctccaa aaccaggaga agccgaggct cccccaaaac 1321 atccaggagt gctgaaagtg gaagccatcc tggagaaggt gcaggggctg gagcaggctg 1381 tagacaactt tgaaggcaag aagactgaca aaaagtacct gatgatcgaa gagtatttga 1441 ccaaagagct gctggccctg gattcagtgg accccgaggg acgagccgat gtgcgtcagg 1501 ccaggagaga cggtgtcagg aaggttcaga ccatcttgga aaaacttgaa cagaaagcca 1561 ttgatgtccc aggtcaagtc caggtctatg aactccagcc cagcaacctt gaagcagatc 1621 agccactgca ggcaatcatg gagatgggtg ccgtggcagc agacaagggc aagaaaaatg 1681 ctggaaatgc agaagatccc cacacagaaa cccagcagcc agaagccaca gcagcagcga 1741 cttcaaaccc cagcagcatg acagacaccc ctggtaaccc agcagcaccg tnnccaactt 1801 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 1861 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta