Construct: ORF ccsbBroad304_15742
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011577.1_s304c1, BRDN0000405940
- Derived from:
- ccsbBroadEn_15742
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GABARAPL2 (11345)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15742
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11345 | GABARAPL2 | GABA type A receptor associ... | NM_007285.7 | 99.7% | 100% | 153T>C |
| 2 | mouse | 93739 | Gabarapl2 | gamma-aminobutyric acid (GA... | NM_026693.5 | 93.1% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 417
- ORF length:
- 351
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa gtggatgttc aaggaggacc actcgctgga acacagatgc gtggagtccg 121 cgaagattcg agcgaaatat cccgacaggg ttccggtgat tgtggaaaag gtctcaggct 181 ctcagattgt tgacattgac aaacggaagt acttggtccc atctgatatc actgtggctc 241 agttcatgtg gatcatcagg aaaaggatcc agcttccttc tgaaaaggcg atcttcctgt 301 ttgtggataa gacagtccca cagtccagcc taactatggg acagctttac gagaaggaaa 361 aagatgaaga tggattctta tatgtggcct acagcggaga gaacactttt ggcttctnnc 421 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 481 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 541 tta