Construct: ORF ccsbBroad304_15812

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009272.1_s304c1, BRDN0000406163
Derived from:
ccsbBroadEn_15812
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DCAF8 (50717)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15812
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 50717 DCAF8 DDB1 and CUL4 associated fa... NR_028106.1 54.7% 1_279del;483C>G;1099_1495del
2 human 50717 DCAF8 DDB1 and CUL4 associated fa... NR_028105.1 47.3% 1_512del;716C>G;1332_1728del
3 human 50717 DCAF8 DDB1 and CUL4 associated fa... NM_015726.4 44.3% 41.6% (many diffs)
4 human 50717 DCAF8 DDB1 and CUL4 associated fa... NR_028104.2 19.2% (many diffs)
5 human 50717 DCAF8 DDB1 and CUL4 associated fa... NR_028103.2 18.9% (many diffs)
6 mouse 98193 Dcaf8 DDB1 and CUL4 associated fa... NM_153555.2 39.1% 37.9% (many diffs)
7 mouse 98193 Dcaf8 DDB1 and CUL4 associated fa... XM_006497065.3 39.1% 37.9% (many diffs)
8 mouse 98193 Dcaf8 DDB1 and CUL4 associated fa... XM_011238889.2 39.1% 37.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
885
ORF length:
819
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtc cagcaaaggg agcagcacag atggcagaac agacttagct aatggaagcc
121tgtctagcag tccagaggag atgtctggag ctgaagaggg gagggagaca tcctcaggca
181ttgaagtgga ggcctcagac ctgagtttga gcttgactgg ggatgatggt ggccccaacc
241gcaccagcac agaaagtcga ggcacagaga cagagagctc aggtgaagat aaggactctg
301acagcatgga ggacactggt cattactcca ttaatgatga aaatcgagtc catgaccgct
361cagaggaaga ggaagaggag gaagaagagg aggaagaaga gcagcctcgg cgccgtgtac
421agcgcaagcg ggctaaccgt gaccaggact catcagatga tgagcgggcc ctagaggact
481gggtgtcctc agaaacatca gctctacccc gacctcgctg gcaagccctc cctgcccttc
541gggagcggga gctgggttca agtgcccgct ttgtctatga ggcctgtggg gcaagagtct
601ttgtgcagcg tttccgcctg cagcatgggc ttgagggcca tactggttgt gtcaataccc
661tgcactttaa ccagcgcggc acctggctgg ccagtggcag cgatgacctg aaggtggtgg
721tgtgggattg ggtacggcgg cagccagtac tggactttga gagtggccac aaaagtaatg
781tgttccaggt gaggcaaggg agcataatag caactgagag aatcaggcat gagttagaga
841aatctgaact gcagcatgga ttgggagctg atagtgagtt attgtnncca actttcttgt
901acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat
961gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a

Download FASTA (ORF) (Full)