Transcript: Human NR_028104.2

Homo sapiens DDB1 and CUL4 associated factor 8 (DCAF8), transcript variant 3, non-coding RNA.

Source:
NCBI, updated 2019-07-31
Taxon:
Homo sapiens (human)
Gene:
DCAF8 (50717)
Length:
4125
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_028104.2
NBCI Gene record:
DCAF8 (50717)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_028104.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000242442 TCACTGTTAGAGGCCTAATTT pLKO_005 3050 3UTR 100% 15.000 21.000 N DCAF8 n/a
2 TRCN0000242444 GCCATACTGGTTGTGTCAATA pLKO_005 1031 3UTR 100% 13.200 18.480 N DCAF8 n/a
3 TRCN0000250208 GCCATACTGGTTGTGTCAATA pLKO_005 1031 3UTR 100% 13.200 18.480 N Dcaf8 n/a
4 TRCN0000242446 ACTCTCCCTGTACGTTCTTAT pLKO_005 1340 3UTR 100% 13.200 10.560 N DCAF8 n/a
5 TRCN0000242443 GGCAGAACAGACTTAGCTAAT pLKO_005 487 3UTR 100% 10.800 8.640 N DCAF8 n/a
6 TRCN0000242445 ATGGAGGACACTGGTCATTAC pLKO_005 700 3UTR 100% 10.800 7.560 N DCAF8 n/a
7 TRCN0000172323 CCATCCAACTTCATGCCAGTT pLKO.1 3591 3UTR 100% 4.050 2.835 N DCAF8 n/a
8 TRCN0000167447 GAACAGACTTAGCTAATGGAA pLKO.1 491 3UTR 100% 3.000 2.100 N DCAF8 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_028104.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03151 pDONR223 100% 43.4% None 1_459del;2251_4125del n/a
2 ccsbBroad304_03151 pLX_304 0% 43.4% V5 1_459del;2251_4125del n/a
3 TRCN0000468372 TCTATGGCCACTTGTCGCGATTAA pLX_317 23.2% 43.4% V5 1_459del;2251_4125del n/a
4 ccsbBroadEn_15812 pDONR223 0% 19.2% None (many diffs) n/a
5 ccsbBroad304_15812 pLX_304 0% 19.2% V5 (many diffs) n/a
6 TRCN0000470465 AGCGCATCGCTCAATGGACGGCCG pLX_317 42.6% 19.2% V5 (many diffs) n/a
Download CSV