Construct: ORF ccsbBroad304_15815

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002730.1_s304c1, BRDN0000399011
Derived from:
ccsbBroadEn_15815
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SPOCK3 (50859)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15815
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 50859 SPOCK3 SPARC (osteonectin), cwcv a... NM_001040159.2 99.9% 100% 147T>C
2 human 50859 SPOCK3 SPARC (osteonectin), cwcv a... XM_017008258.1 99.9% 100% 147T>C
3 human 50859 SPOCK3 SPARC (osteonectin), cwcv a... NM_016950.2 99.2% 99.3% 147T>C;190_198delGAAGTAGAG
4 human 50859 SPOCK3 SPARC (osteonectin), cwcv a... XM_011532018.1 99.2% 99.3% 147T>C;190_198delGAAGTAGAG
5 human 50859 SPOCK3 SPARC (osteonectin), cwcv a... XM_017008257.1 97.2% 97.3% 1_36del;183T>C
6 human 50859 SPOCK3 SPARC (osteonectin), cwcv a... NM_001204356.1 90.6% 90.5% 147T>C;589_590ins120
7 human 50859 SPOCK3 SPARC (osteonectin), cwcv a... NM_001204352.1 87% 85.9% (many diffs)
8 human 50859 SPOCK3 SPARC (osteonectin), cwcv a... NM_001204354.1 79.4% 79.4% 0_1ins267
9 human 50859 SPOCK3 SPARC (osteonectin), cwcv a... NM_001204355.1 78.4% 72.7% 0_1ins118;29T>C;71_72ins161
10 human 50859 SPOCK3 SPARC (osteonectin), cwcv a... NM_001251967.1 77.9% 78% 147T>C;189_190ins285
11 human 50859 SPOCK3 SPARC (osteonectin), cwcv a... NM_001204353.1 72.9% 72.9% 0_1ins351
12 human 50859 SPOCK3 SPARC (osteonectin), cwcv a... NM_001204358.2 71.6% 71.5% (many diffs)
13 mouse 72902 Spock3 sparc/osteonectin, cwcv and... NM_001252621.1 89.3% 90% (many diffs)
14 mouse 72902 Spock3 sparc/osteonectin, cwcv and... XM_006509518.1 89.3% 90% (many diffs)
15 mouse 72902 Spock3 sparc/osteonectin, cwcv and... NM_001252620.1 88.7% 89.4% (many diffs)
16 mouse 72902 Spock3 sparc/osteonectin, cwcv and... NM_023689.3 88.7% 89.4% (many diffs)
17 mouse 72902 Spock3 sparc/osteonectin, cwcv and... XM_006509517.2 86.1% 86.8% (many diffs)
18 mouse 72902 Spock3 sparc/osteonectin, cwcv and... XM_006509516.2 85.6% 86.2% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1365
ORF length:
1299
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgct caaggtgtca gccgtactgt gtgtgtgtgc agccgcttgg tgcagtcagt
121ctctcgcagc tgccgcggcg gtggctgcag ccggggggcg gtcggacggc ggtaattttc
181tggatgataa acaatggctc accacaatct cccagtatga caaggaagtc ggacagtgga
241acaaattccg agacgatgat tatttccgca cttggagtcc aggaaaaccc ttcgatcagg
301ctttagatcc agctaaggat ccatgcttaa agatgaaatg tagtcgccat aaagtatgca
361ttgctcaaga ttctcagact gcagtctgca ttagtcaccg gaggcttaca cacaggatga
421aagaagcagg agtagaccat aggcagtgga ggggtcccat attatccacc tgcaagcagt
481gcccagtggt ctatcccagc cctgtttgtg gttcagatgg tcatacctac tcttttcagt
541gcaaactaga atatcaggca tgtgtcttag gaaaacagat ctcagtcaaa tgtgaaggac
601attgcccatg tccttcagat aagcccacca gtacaagcag aaatgttaag agagcatgca
661gtgacctgga gttcagggaa gtggcaaaca gattgcggga ctggttcaag gcccttcatg
721aaagtggaag tcaaaacaag aagacaaaaa cattgctgag gcctgagaga agcagattcg
781ataccagcat cttgccaatt tgcaaggact cacttggctg gatgtttaac agacttgata
841caaactatga cctgctattg gaccagtcag agctcagaag catttacctt gataagaatg
901aacagtgtac caaggcattc ttcaattctt gtgacacata caaggacagt ttaatatcta
961ataatgagtg gtgctactgc ttccagagac agcaagaccc accttgccag actgagctca
1021gcaatattca gaagcggcaa ggggtaaaga agctcctagg acagtatatc cccctgtgtg
1081atgaagatgg ttactacaag ccaacacaat gtcatggcag tgttggacag tgctggtgtg
1141ttgacagata tggaaatgaa gtcatgggat ccagaataaa tggtgttgca gattgtgcta
1201tagattttga gatctccgga gattttgcta gtggcgattt tcatgaatgg actgatgatg
1261aggatgatga agacgatatt atgaatgatg aagatgaaat tgaagatgat gatgaagatg
1321aaggggatga tgatgatggt ggtgatgacc atgatgtata catttnncca actttcttgt
1381acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat
1441gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a

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