Construct: ORF ccsbBroad304_15849

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016829.1_s304c1, BRDN0000399574
Derived from:
ccsbBroadEn_15849
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SIRT6 (51548)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15849
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51548 SIRT6 sirtuin 6 NM_001193285.3 99.8% 99.6% 137G>A
2 human 51548 SIRT6 sirtuin 6 NM_016539.4 92.3% 92.1% 137G>A;530_610del
3 human 51548 SIRT6 sirtuin 6 XM_024451539.1 79.5% 71% (many diffs)
4 human 51548 SIRT6 sirtuin 6 NM_001321061.2 78% 78% 0_1ins216
5 human 51548 SIRT6 sirtuin 6 NM_001321059.2 75.1% 74.9% 137G>A;191_192ins183;347_427del
6 human 51548 SIRT6 sirtuin 6 NM_001321058.2 72.1% 72.1% 0_1ins216;314_394del
7 human 51548 SIRT6 sirtuin 6 NM_001321060.2 67% 50% (many diffs)
8 human 51548 SIRT6 sirtuin 6 NM_001321062.2 54.3% 54.3% 0_1ins405;125_205del
9 human 51548 SIRT6 sirtuin 6 NM_001321063.2 49.2% 33.5% (many diffs)
10 human 51548 SIRT6 sirtuin 6 NM_001321064.1 46.1% 30.8% (many diffs)
11 human 51548 SIRT6 sirtuin 6 XM_024451540.1 46.1% 30.8% (many diffs)
12 mouse 50721 Sirt6 sirtuin 6 NM_181586.3 72.2% 75.7% (many diffs)
13 mouse 50721 Sirt6 sirtuin 6 XM_006513861.4 62.5% 65.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1050
ORF length:
984
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtc ggtgaattac gcggcggggc tgtcgccgta cgcggacaag ggcaagtgcg
121gcctcccgga gatcttcgac cccccggagg agctggagcg gaaggtgtgg gaactggcga
181ggctggtctg gcagtcttcc aatgtggtgt tccacacggg tgccggcatc agcactgcct
241ctggcatccc cgacttcagg ggtccccacg gagtctggac catggaggag cgaggtctgg
301cccccaagtt cgacaccacc tttgagagcg cgcggcccac gcagacccac atggcgctgg
361tgcagctgga gcgcgtgggc ctcctccgct tcctggtcag ccagaacgtg gacgggctcc
421atgtgcgctc aggcttcccc agggacaaac tggcagagct ccacgggaac atgtttgtgg
481aagaatgtgc caagtgtaag acgcagtacg tccgagacac agtcgtgggc accatgggcc
541tgaaggccac gggccggctc tgcaccgtgg ctaaggcaag ggggctgcga gcctgcagga
601acgccgacct gtccatcacg ctgggtacat cgctgcagat ccggcccagc gggaacctgc
661cgctggctac caagcgccgg ggaggccgcc tggtcatcgt caacctgcag cccaccaagc
721acgaccgcca tgctgacctc cgcatccatg gctacgttga cgaggtcatg acccggctca
781tgaagcacct ggggctggag atccccgcct gggacggccc ccgtgtgctg gagagggcgc
841tgccacccct gccccgcccg cccaccccca agctggagcc caaggaggaa tctcccaccc
901ggatcaacgg ctctatcccc gccggcccca agcaggagcc ctgcgcccag cacaacggct
961cagagcccgc cagccccaaa cgggagcggc ccaccagccc tgccccccac agacccccca
1021aaagggtgaa ggccaaggcg gtccccagct nnccaacttt cttgtacaaa gtggttggta
1081agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc
1141ggtggcgcgt taagtcgaca atcaacctct ggatta

Download FASTA (ORF) (Full)