Transcript: Human XM_024451539.1

PREDICTED: Homo sapiens sirtuin 6 (SIRT6), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
SIRT6 (51548)
Length:
1653
CDS:
150..1145

Additional Resources:

NCBI RefSeq record:
XM_024451539.1
NBCI Gene record:
SIRT6 (51548)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024451539.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000232530 GCTACGTTGACGAGGTCATGA pLKO_005 844 CDS 100% 4.950 6.930 N SIRT6 n/a
2 TRCN0000232531 ACCCGGATCAACGGCTCTATC pLKO_005 990 CDS 100% 3.600 5.040 N SIRT6 n/a
3 TRCN0000368637 CAAGTGTAAGACGCAGTACGT pLKO_005 503 CDS 100% 2.640 3.696 N SIRT6 n/a
4 TRCN0000050476 CACCCGGATCAACGGCTCTAT pLKO.1 989 CDS 100% 1.650 2.310 N SIRT6 n/a
5 TRCN0000378253 CAGTACGTCCGAGACACAGTC pLKO_005 516 CDS 100% 1.350 1.890 N SIRT6 n/a
6 TRCN0000378369 ACGGGAACATGTTTGTGGAAG pLKO_005 475 CDS 100% 4.050 3.240 N SIRT6 n/a
7 TRCN0000232532 CTCCCTGGTCTCCAGCTTAAA pLKO_005 1422 3UTR 100% 13.200 9.240 N SIRT6 n/a
8 TRCN0000232528 GAAGAATGTGCCAAGTGTAAG pLKO_005 492 CDS 100% 10.800 7.560 N SIRT6 n/a
9 TRCN0000358693 CAAGTTCGACACCACCTTTGA pLKO_005 317 CDS 100% 4.950 3.465 N SIRT6 n/a
10 TRCN0000050473 TGGAAGAATGTGCCAAGTGTA pLKO.1 490 CDS 100% 4.950 3.465 N SIRT6 n/a
11 TRCN0000232529 TGTCCATCACGCTGGGTACAT pLKO_005 703 CDS 100% 4.950 3.465 N SIRT6 n/a
12 TRCN0000050474 GCAGTCTTCCAGTGTGGTGTT pLKO.1 1 5UTR 100% 4.050 2.835 N SIRT6 n/a
13 TRCN0000050477 CACGGGAACATGTTTGTGGAA pLKO.1 474 CDS 100% 2.640 1.848 N SIRT6 n/a
14 TRCN0000050475 GCTGGGTACATCGCTGCAGAT pLKO.1 713 CDS 100% 1.350 0.945 N SIRT6 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024451539.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03332 pDONR223 100% 86.9% 78.2% None (many diffs) n/a
2 ccsbBroad304_03332 pLX_304 0% 86.9% 78.2% V5 (many diffs) n/a
3 TRCN0000471239 GCAATTGCAGTTCTACACTCACTC pLX_317 44.8% 86.9% 78.2% V5 (many diffs) n/a
4 ccsbBroadEn_15849 pDONR223 0% 79.5% 71% None (many diffs) n/a
5 ccsbBroad304_15849 pLX_304 0% 79.5% 71% V5 (many diffs) n/a
6 TRCN0000473167 AATCTGCCTAGTCGGATTGATCCC pLX_317 44.3% 79.4% 71% V5 (many diffs) n/a
7 ccsbBroadEn_11997 pDONR223 100% 77.2% 77% None 1_144del;458_538del;673C>T n/a
8 ccsbBroad304_11997 pLX_304 0% 77.2% 77% V5 1_144del;458_538del;673C>T n/a
9 TRCN0000481003 AGGACAACCTCTTTTTGTCCTATG pLX_317 34.1% 77.2% 77% V5 1_144del;458_538del;673C>T n/a
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