Construct: ORF ccsbBroad304_15858
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017699.1_s304c1, BRDN0000403882
- Derived from:
- ccsbBroadEn_15858
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CLIC5 (53405)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15858
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 53405 | CLIC5 | chloride intracellular chan... | XR_926258.3 | 4.3% | 1_3390del;3598_4773del | |
| 2 | human | 53405 | CLIC5 | chloride intracellular chan... | NR_045672.1 | 3.8% | 1_3930del;4138_5330del | |
| 3 | human | 53405 | CLIC5 | chloride intracellular chan... | NR_045673.1 | 3.8% | 1_4004del;4212_5404del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 273
- ORF length:
- 207
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc cctgtcctca gcattccctt cttgtgctgg tggggccagt gaagtcttga 121 tcttatcaga aaaaggccac accaagtgcg agttttccca ggctgacttt ccaggccctt 181 atcaaatgaa acaacagaag ctcttcacag ttctgtgccc catggccact ccacagacag 241 acaataccaa gcatcttaga actgtcataa gatnnccaac tttcttgtac aaagtggttg 301 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 361 accggtggcg cgttaagtcg acaatcaacc tctggatta