Construct: ORF ccsbBroad304_15887
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018007.1_s304c1, BRDN0000402728
- Derived from:
- ccsbBroadEn_15887
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LRRC8D (55144)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15887
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55144 | LRRC8D | leucine rich repeat contain... | NM_001134479.2 | 16.5% | 16% | (many diffs) |
| 2 | human | 55144 | LRRC8D | leucine rich repeat contain... | NM_018103.5 | 16.5% | 16% | (many diffs) |
| 3 | human | 55144 | LRRC8D | leucine rich repeat contain... | XM_011541689.1 | 16.5% | 16% | (many diffs) |
| 4 | human | 55144 | LRRC8D | leucine rich repeat contain... | XM_017001601.2 | 16.5% | 16% | (many diffs) |
| 5 | human | 55144 | LRRC8D | leucine rich repeat contain... | XM_017001602.1 | 16.5% | 16% | (many diffs) |
| 6 | mouse | 231549 | Lrrc8d | leucine rich repeat contain... | NM_001122768.1 | 14.1% | 16% | (many diffs) |
| 7 | mouse | 231549 | Lrrc8d | leucine rich repeat contain... | NM_178701.3 | 14.1% | 16% | (many diffs) |
| 8 | mouse | 231549 | Lrrc8d | leucine rich repeat contain... | XM_006534893.2 | 14.1% | 16% | (many diffs) |
| 9 | mouse | 231549 | Lrrc8d | leucine rich repeat contain... | XM_006534894.3 | 14.1% | 16% | (many diffs) |
| 10 | mouse | 231549 | Lrrc8d | leucine rich repeat contain... | XM_006534895.3 | 14.1% | 16% | (many diffs) |
| 11 | mouse | 231549 | Lrrc8d | leucine rich repeat contain... | XM_006534896.3 | 14.1% | 16% | (many diffs) |
| 12 | mouse | 231549 | Lrrc8d | leucine rich repeat contain... | XM_011249436.1 | 14.1% | 16% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 495
- ORF length:
- 429
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt cagtagcaac ttttggttca aatatcccaa aacatgctca aaagtagaac 121 attttgtttc aatattagga aagtgctttg aatccccttg gacgacaaaa gcgttgtctg 181 agacagcatg cgaagactca gaggaaaaca agcagagaat aacaggtgcc cagactctac 241 caaagcatgt ttctaccagc agtgatgaag ggagccccag tgccagtaca ccaatgatca 301 ataaaactgg ctttaaattt tcagctgaga agcctgtgat tgaagttccc agcatgacaa 361 tcctggataa aaaggatgga gagcaggcca aagccctgtt tgagaaagtg aggaagttcc 421 gtgcccatgt ggaagatagt gacttgatct ataaactcta tgtggtccaa acagcttctc 481 cctttcccaa ccaatnncca actttcttgt acaaagtggt tggtaagcct atccctaacc 541 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 601 cgacaatcaa cctctggatt a