Transcript: Mouse XM_006534895.3

PREDICTED: Mus musculus leucine rich repeat containing 8D (Lrrc8d), transcript variant X3, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Lrrc8d (231549)
Length:
4451
CDS:
962..3541

Additional Resources:

NCBI RefSeq record:
XM_006534895.3
NBCI Gene record:
Lrrc8d (231549)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006534895.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000217373 GATGCTCACTTGCAAGTTATT pLKO.1 3596 3UTR 100% 13.200 18.480 N Lrrc8d n/a
2 TRCN0000193605 GCCAAACTCATAATCTTAGAT pLKO.1 4087 3UTR 100% 5.625 7.875 N Lrrc8d n/a
3 TRCN0000194188 GCTGTACTTGATCGGCAATTT pLKO.1 2656 CDS 100% 13.200 9.240 N Lrrc8d n/a
4 TRCN0000217179 CCACGTGAAGAGTAATCTAAC pLKO.1 2743 CDS 100% 10.800 7.560 N Lrrc8d n/a
5 TRCN0000147226 GAAGTCAAAGAGGCATTGAAT pLKO.1 3485 CDS 100% 5.625 3.938 N LRRC8D n/a
6 TRCN0000276209 GAAGTCAAAGAGGCATTGAAT pLKO_005 3485 CDS 100% 5.625 3.938 N LRRC8D n/a
7 TRCN0000193931 CCATCATCCTTATGGTCAGTA pLKO.1 1482 CDS 100% 4.950 3.465 N Lrrc8d n/a
8 TRCN0000175169 GCATTGAATCAAGATGTCAAT pLKO.1 3497 CDS 100% 0.495 0.347 N Lrrc8d n/a
9 TRCN0000174975 GAAGACAGTGACTTGATCTAT pLKO.1 1859 CDS 100% 5.625 3.375 N Lrrc8d n/a
10 TRCN0000091448 CCTCCCAAGTACTGGGATTAA pLKO.1 455 5UTR 100% 1.320 0.660 Y Epb42 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006534895.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15887 pDONR223 0% 14.1% 16% None (many diffs) n/a
2 ccsbBroad304_15887 pLX_304 0% 14.1% 16% V5 (many diffs) n/a
3 TRCN0000473967 CGATAAACCCCATATGAGTACGCC pLX_317 100% 14.1% 16% V5 (many diffs) n/a
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