Construct: ORF ccsbBroad304_15891
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004502.1_s304c1, BRDN0000405430
- Derived from:
- ccsbBroadEn_15891
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCMTD2 (55251)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15891
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55251 | PCMTD2 | protein-L-isoaspartate (D-a... | NM_018257.3 | 72.2% | 69.5% | 1_164del;203_338del |
| 2 | human | 55251 | PCMTD2 | protein-L-isoaspartate (D-a... | NM_001104925.1 | 64.8% | 62% | 1_164del;203_338del;580_581ins81 |
| 3 | mouse | 245867 | Pcmtd2 | protein-L-isoaspartate (D-a... | XM_006500626.3 | 33.7% | 32.9% | (many diffs) |
| 4 | mouse | 245867 | Pcmtd2 | protein-L-isoaspartate (D-a... | XM_006500627.3 | 33.4% | 32.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 852
- ORF length:
- 783
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaagcatgg aaacattcac ctctcagccc cgtgcatcac tcagatgtga 121 tagagtatgc aaagcagaaa ctggacttct tcatcagaac aagtgatagt tttgacaagt 181 ttgacttctg tgaaccttcc tttgttactg ggaattgcct ggagatttct ccggattgtt 241 ctcagtatga tcgtgtatac tgtggggctg gcgtgcagaa agagcatgaa gagtacatga 301 agaatcttct caaagtggga gggatccttg tcatgccact ggaagagaag ttgactaaga 361 taacacgcac aggtccttca gcttgggaaa ccaaaaagat tcttgctgtt tcttttgctc 421 ctctgatcca gccctgccat tcagagtcag gaaaatcaag acttgtccag ttaccaccag 481 tggcagttcg cagcctccag gacttggctc gcatcgccat ccggggcacc attaaaaaga 541 ttattcatca ggaaactgtg agcaaaaacg gaaacggact aaagaacacc cccaggttta 601 aacgaaggag agttcgccgc cgtcgaatgg aaacgattgt ctttttggac aaagaagtct 661 ttgccagtcg gatttccaac ccctcagatg acaacagctg tgaagacttg gaagaggaac 721 ggagggaaga agaagagaag accccgccgg aaacaaagcc agacccccca gtgaacttcc 781 tacgccagaa ggtcctgagc ctccctctgc cagatcccct gaaatactac ttgctttatt 841 acagagaaaa atnnccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 901 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 961 caatcaacct ctggatta