Construct: ORF ccsbBroad304_15895

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003500.1_s304c1, BRDN0000408663
Derived from:
ccsbBroadEn_15895
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RHOT1 (55288)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15895
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55288 RHOT1 ras homolog family member T1 NM_018307.4 100% 100%
2 human 55288 RHOT1 ras homolog family member T1 NM_001288755.1 96.6% 96.6% 0_1ins63
3 human 55288 RHOT1 ras homolog family member T1 XM_017024825.1 96.6% 96.6% 0_1ins63
4 human 55288 RHOT1 ras homolog family member T1 XM_011524976.2 95.1% 94.3% (many diffs)
5 human 55288 RHOT1 ras homolog family member T1 NM_001288754.1 95% 95% 1740_1835del
6 human 55288 RHOT1 ras homolog family member T1 XM_017024823.1 94.2% 92.4% (many diffs)
7 human 55288 RHOT1 ras homolog family member T1 NM_001033566.2 93.7% 93.7% 1740_1862del
8 human 55288 RHOT1 ras homolog family member T1 XM_017024821.1 92.4% 90.2% (many diffs)
9 human 55288 RHOT1 ras homolog family member T1 XM_017024819.1 92.1% 91.9% (many diffs)
10 human 55288 RHOT1 ras homolog family member T1 XM_017024824.1 91.8% 91.8% 0_1ins63;1677_1772del
11 human 55288 RHOT1 ras homolog family member T1 XM_011524973.2 91.7% 89.3% (many diffs)
12 human 55288 RHOT1 ras homolog family member T1 XM_011524971.2 90.8% 88.2% (many diffs)
13 human 55288 RHOT1 ras homolog family member T1 XM_017024820.1 90.7% 89.7% (many diffs)
14 human 55288 RHOT1 ras homolog family member T1 XM_017024822.1 90.5% 90.5% 0_1ins63;1677_1799del
15 human 55288 RHOT1 ras homolog family member T1 NM_001033568.2 89.4% 89.4% 1740_1958del
16 human 55288 RHOT1 ras homolog family member T1 XM_011524975.3 86.3% 86.3% 0_1ins63;1677_1895del
17 human 55288 RHOT1 ras homolog family member T1 XM_024450827.1 86.3% 86.3% 0_1ins63;1677_1895del
18 human 55288 RHOT1 ras homolog family member T1 XM_011524969.2 85.6% 83.2% (many diffs)
19 human 55288 RHOT1 ras homolog family member T1 NM_001033567.2 79.4% 79.4% 0_1ins381
20 human 55288 RHOT1 ras homolog family member T1 NM_001288758.1 75.5% 75.5% 0_1ins381;1359_1454del
21 human 55288 RHOT1 ras homolog family member T1 XR_934499.2 63.1% 1_35del;1572_1623del;1942_2938del
22 human 55288 RHOT1 ras homolog family member T1 XR_001752556.1 60.5% (many diffs)
23 human 55288 RHOT1 ras homolog family member T1 XR_001752555.1 59.3% 1_35del;1775_2013del;2129_3125del
24 human 55288 RHOT1 ras homolog family member T1 XR_001752554.1 57% 0_1ins37;1703_2037del;2153_3149del
25 human 55288 RHOT1 ras homolog family member T1 NR_110083.1 54.5% 1_239del;1979_2239del;2355_3401del
26 mouse 59040 Rhot1 ras homolog family member T1 NM_021536.7 90.3% 95.8% (many diffs)
27 mouse 59040 Rhot1 ras homolog family member T1 NM_001163354.1 86% 91.2% (many diffs)
28 mouse 59040 Rhot1 ras homolog family member T1 NM_001163355.1 84.8% 90% (many diffs)
29 mouse 59040 Rhot1 ras homolog family member T1 XM_011249168.2 81% 85.9% (many diffs)
30 mouse 59040 Rhot1 ras homolog family member T1 XM_011249167.2 79.2% 84% (many diffs)
31 mouse 59040 Rhot1 ras homolog family member T1 XM_006533886.3 75.8% 80.4% (many diffs)
32 mouse 59040 Rhot1 ras homolog family member T1 XR_879726.2 69.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1923
ORF length:
1854
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gaagaaagac gtgcggatcc tgctggtggg agaacctaga gttgggaaga
121catcactgat tatgtctctg gtcagtgaag aatttccaga agaggttcct ccccgggcag
181aagaaatcac cattccagct gatgtcaccc cagagagagt tccaacacac attgtagatt
241actcagaagc agaacagagt gatgaacaac ttcatcaaga aatatctcag gctaatgtca
301tctgtatagt gtatgccgtt aacaacaagc attctattga taaggtaaca agtcgatgga
361ttcctctcat aaatgaaaga acagacaaag acagcaggct gcctttaata ttggttggga
421acaaatctga tctggtggaa tatagtagta tggagaccat ccttcctatt atgaaccagt
481atacagaaat agaaacctgt gtggagtgtt cagcgaaaaa cctgaagaac atatcagagc
541tcttttatta cgcacagaaa gctgttcttc atcctacagg gcccctgtac tgcccagagg
601agaaggagat gaaaccagct tgtataaaag cccttactcg tatatttaaa atatctgatc
661aagataatga tggtactctc aatgatgctg aactcaactt ctttcagagg atttgtttca
721acactccatt agctcctcaa gctctggagg atgtcaagaa tgtagtcaga aaacatataa
781gtgatggtgt ggctgacagt gggttgaccc tgaaaggttt tctcttttta cacacacttt
841ttatccagag agggagacac gaaactactt ggactgtgct tcgacgattt ggttatgatg
901atgacctgga tttgacacct gaatatttgt tccccctgct gaaaatacct cctgattgca
961ctactgaatt aaatcatcat gcatatttat ttctccaaag cacctttgac aagcatgatt
1021tggatagaga ctgtgctttg tcacctgatg agcttaaaga tttatttaaa gttttccctt
1081acataccttg ggggccagat gtgaataaca cagtttgtac caatgaaaga ggctggataa
1141cctaccaggg attcctttcc cagtggacgc tcacgactta tttagatgta cagcggtgcc
1201tggaatattt gggctatcta ggctattcaa tattgactga gcaagagtct caagcttcag
1261ctgttacagt gacaagagat aaaaagatag acctgcagaa aaaacaaact caaagaaatg
1321tgttcagatg taatgtaatt ggagtgaaaa actgtgggaa aagtggagtt cttcaggctc
1381ttcttggaag aaacttaatg aggcagaaga aaattcgtga agatcataaa tcctactatg
1441cgattaacac tgtttatgta tatggacaag agaaatactt gttgttgcat gatatctcag
1501aatcggaatt tctaactgaa gctgaaatca tttgtgatgt tgtatgcctg gtatatgatg
1561tcagcaatcc caaatccttt gaatactgtg ccaggatttt taagcaacac tttatggaca
1621gcagaatacc ttgcttaatc gtagctgcaa agtcagacct gcatgaagtt aaacaagaat
1681acagtatttc acctactgat ttctgcagga aacacaaaat gcctccacca caagccttca
1741cttgcaatac tgctgatgcc cccagtaagg atatctttgt taaattgaca acaatggcca
1801tgtatccgca cgtgacacaa gctgacctca agagctccac gttttggctt cgagcaagtt
1861ttggtgctac tgtttttgca gttttgggct ttgctatgta caaagcatta ttgaaacagc
1921gatnnccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc
1981tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc
2041tctggatta

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