Construct: ORF ccsbBroad304_15908
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002745.1_s304c1, BRDN0000410015
- Derived from:
- ccsbBroadEn_15908
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PEX26 (55670)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15908
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55670 | PEX26 | peroxisomal biogenesis fact... | NM_001127649.3 | 99.8% | 99.6% | 911G>A |
| 2 | human | 55670 | PEX26 | peroxisomal biogenesis fact... | NM_017929.6 | 99.8% | 99.6% | 911G>A |
| 3 | human | 55670 | PEX26 | peroxisomal biogenesis fact... | NM_001199319.1 | 83.8% | 83.6% | 665_666ins147;764G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 981
- ORF length:
- 915
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa gagcgattct tcgacctctg cagcccccct cagggggctc gggggacccc 121 tgcgcagcag cgagccggtg cgcgcggtcc cggcccgggc gccggccgtg gaccttctgg 181 aggaggcggc cgacctcctg gtggtgcacc tggacttccg ggcggcgctg gagacctgcg 241 agcgggcctg gcagagtctg gccaaccacg ccgtggcaga ggaacccgcg ggcacctcat 301 tggaggtgaa gtgctccctg tgtgttgtgg ggatccaggc cctggcagaa atggatcggt 361 ggcaagaagt cctctcctgg gtccttcagt attaccaggt ccctgaaaag ctacccccca 421 aagtcctgga gctgtgcatt cttttataca gcaaaatgca agagcctgga gctgtgctgg 481 atgtggtggg tgcctggctc caagacccag ccaatcaaaa ccttccagaa tatggagcct 541 tggcagaatt tcacgtgcag cgggtgctgc tgcctctggg ctgcttatcg gaggctgagg 601 agctagtggt gggctctgca gcctttggtg aggagcggcg actggatgta cttcaggcca 661 ttcacacagc gaggcagcag cagaaacagg aacactcagg ctctgaggag gcccagaagc 721 caaacctgga aggctctgtc tcccacaagt tcctgtcact accgatgttg gttcgccagc 781 tttgggactc tgcggtgagc cacttctttt ctctgccctt caaaaagagt ctcctggctg 841 ccttgatcct ctgtctcctg gtggtgagat ttgatccagc ttccccttcc tccctgcact 901 tcctctacaa gctggcccag ctcttccgct ggatccggaa ggctgcattt tctcgcctct 961 accagctccg catccatgac tnnccaactt tcttgtacaa agtggttggt aagcctatcc 1021 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 1081 ttaagtcgac aatcaacctc tggatta