Construct: ORF ccsbBroad304_15922

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016092.1_s304c1, BRDN0000404624
Derived from:
ccsbBroadEn_15922
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GSDMC (56169)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15922
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 56169 GSDMC gasdermin C NM_031415.3 99.8% 99.8% 651A>G;1424T>C;1500G>A
2 human 56169 GSDMC gasdermin C XM_011517158.2 98% 97.8% (many diffs)
3 human 56169 GSDMC gasdermin C XM_011517159.2 96% 96% (many diffs)
4 human 56169 GSDMC gasdermin C XM_011517161.2 90.4% 85.6% (many diffs)
5 human 56169 GSDMC gasdermin C XM_011517162.2 85.6% 85.4% (many diffs)
6 human 56169 GSDMC gasdermin C XM_011517163.3 82.8% 82.8% (many diffs)
7 human 56169 GSDMC gasdermin C XM_017013645.1 70.4% 63.3% (many diffs)
8 human 56169 GSDMC gasdermin C XR_928341.2 57.1% (many diffs)
9 human 56169 GSDMC gasdermin C XM_011517165.1 53.1% 52.5% (many diffs)
10 human 56169 GSDMC gasdermin C XR_928340.2 52.3% (many diffs)
11 human 56169 GSDMC gasdermin C XM_011517166.3 51.4% 51.3% 0_1ins738;686T>C;762G>A
12 human 56169 GSDMC gasdermin C XR_928339.2 50.5% (many diffs)
13 human 56169 GSDMC gasdermin C XM_011517167.2 49.6% 47.9% (many diffs)
14 human 56169 GSDMC gasdermin C XM_011517168.2 49.3% 47.8% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1590
ORF length:
1524
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgcc ctccatgttg gaacgcatta gcaaaaattt ggtcaaagag attggaagca
121aagacctgac acctgtcaaa tacctattga gtgccaccaa attacgtcag tttgttatat
181tacgaaagaa gaaggattct cgttcatcat tttgggaaca atctgactat gttccagttg
241aattctccct caatgacatc ctggagccaa gttcttcagt cctagaaact gttgtgacag
301gaccgttcca cttcagtgac attatgatcc agaagcataa ggctgacatg ggtgtgaatg
361ttggtataga agtgagtgtg tcaggggagg cctctgtgga ccatggatgc tccctcgagt
421ttcaaattgt taccatccca tcaccaaacc tggaagactt tcaaaaaagg aaactgttgg
481atccagagcc atcatttctg aaggagtgcc ggaggagagg ggacaacctg tacgtggtga
541cagaggctgt tgaactgatc aacaatactg tgctgtacga tagcagtagt gtgaatattt
601tagggaaaat tgctctttgg attacctatg gcaagggtca aggccaagga gagagtctca
661gagtgaagaa gaaggcgctg actcttcaga aaggcatggt gatggcttat aagaggaagc
721agctggttat caaggagaaa gccattctca tctcagatga tgatgaacag agaacctttc
781aagatgagta cgaaatttcc gaaatggtag gctactgtgc tgcgaggagt gaggggttgc
841taccatcatt tcataccatc tctccaaccc tcttcaatgc ctcatccaat gatatgaagt
901taaaaccaga gctatttctg acacagcaat ttttgagcgg gcatttgcca aaatacgaac
961aagttcacat cctcccagta ggaagaatag aggaaccctt ctggcaaaat ttcaagcatc
1021tacaagagga ggttttccag aaaataaaga cactggctca gctctcaaag gatgttcagg
1081atgtcatgtt ctacagtatc ctggccatgc tcagagacag aggggctcta caggacctga
1141tgaacatgct ggaattggac agctcaggtc atttggatgg ccctggtggt gccatcctaa
1201agaaacttca acaggattca aaccatgcat ggtttaaccc aaaggacccc attctttatc
1261tccttgaagc cataatggtg ctgagtgact tccaacacga tttgctggcc tgttccatgg
1321agaagaggat cctgcttcag caacaggagc tggtaaggag catcctggag ccaaacttca
1381gatacccctg gagcattccc ttcaccctca aacctgagct cctcgcccca ctccagagtg
1441agggtttggc catcacctat ggcctgctgg aggagtgtgg ccttaggacg gagctggata
1501accccaggtc aacctgggat gtagaagcaa agatgcccct gtctgccctc tatgggactc
1561tctcattgct gcagcagctg gctgaggcct nnccaacttt cttgtacaaa gtggttggta
1621agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc
1681ggtggcgcgt taagtcgaca atcaacctct ggatta

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