Construct: ORF ccsbBroad304_16016
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007344.1_s304c1, BRDN0000409013
- Derived from:
- ccsbBroadEn_16016
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LAS1L (81887)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_16016
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81887 | LAS1L | LAS1 like ribosome biogenes... | NM_001170649.1 | 100% | 100% | |
| 2 | human | 81887 | LAS1L | LAS1 like ribosome biogenes... | XM_017029877.2 | 99.8% | 99.8% | 1874_1875insAGA |
| 3 | human | 81887 | LAS1L | LAS1 like ribosome biogenes... | NM_031206.4 | 97.6% | 97.5% | 1043_1093del |
| 4 | human | 81887 | LAS1L | LAS1 like ribosome biogenes... | XM_005262301.2 | 97.5% | 97.4% | 1043_1093del;1925_1926insAGA |
| 5 | human | 81887 | LAS1L | LAS1 like ribosome biogenes... | NM_001170650.1 | 94.1% | 94.1% | 233_234ins126 |
| 6 | human | 81887 | LAS1L | LAS1 like ribosome biogenes... | XM_011531045.2 | 91.9% | 91.8% | 233_234ins126;917_967del |
| 7 | human | 81887 | LAS1L | LAS1 like ribosome biogenes... | XR_001755730.2 | 85.1% | 1_63del;1461_1576del;2331_2526del | |
| 8 | human | 81887 | LAS1L | LAS1 like ribosome biogenes... | XR_244504.2 | 83.4% | (many diffs) | |
| 9 | human | 81887 | LAS1L | LAS1 like ribosome biogenes... | XR_430522.2 | 83.3% | (many diffs) | |
| 10 | human | 81887 | LAS1L | LAS1 like ribosome biogenes... | XR_938412.2 | 72% | (many diffs) | |
| 11 | human | 81887 | LAS1L | LAS1 like ribosome biogenes... | XM_005262304.2 | 67.9% | 60.5% | (many diffs) |
| 12 | human | 81887 | LAS1L | LAS1 like ribosome biogenes... | XM_017029878.2 | 65.5% | 65.1% | (many diffs) |
| 13 | human | 81887 | LAS1L | LAS1 like ribosome biogenes... | XM_011531046.2 | 64.3% | 64.3% | (many diffs) |
| 14 | human | 81887 | LAS1L | LAS1 like ribosome biogenes... | XM_005262306.4 | 64% | 63.4% | (many diffs) |
| 15 | human | 81887 | LAS1L | LAS1 like ribosome biogenes... | XM_005262305.4 | 64% | 63.4% | (many diffs) |
| 16 | human | 81887 | LAS1L | LAS1 like ribosome biogenes... | XM_017029880.2 | 57.8% | 55.2% | 0_1ins796;49_50ins110 |
| 17 | human | 81887 | LAS1L | LAS1 like ribosome biogenes... | XM_017029879.2 | 56.5% | 53.8% | 0_1ins796;49_50ins110;137_187del |
| 18 | human | 81887 | LAS1L | LAS1 like ribosome biogenes... | XR_001755731.2 | 53.1% | (many diffs) | |
| 19 | human | 81887 | LAS1L | LAS1 like ribosome biogenes... | XR_001755733.2 | 53% | 1_63del;1461_3107del;3862_4057del | |
| 20 | human | 81887 | LAS1L | LAS1 like ribosome biogenes... | XR_001755732.2 | 52.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2217
- ORF length:
- 2151
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc gtgggaatcc ggggccgggc caggtctagg ttcccagggg atggatctcg 121 tgtggagtgc gtggtacgga aagtgcgtta aagggaaagg gtcgttgcca ctctcggccc 181 acggcatcgt ggtcgcctgg ctcagcaggg ccgagtggga ccaggtgacg gtttatctgt 241 tctgtgacga ccataagttg cagcggtacg cgcttaaccg catcacggtg tggaggagca 301 ggtcaggcaa cgaactccct ctggcagtgg cttctactgc tgacctgata cgctgtaagc 361 tcttggatgt aactggtggc ttgggcactg atgaacttag actgctctat ggcatggcat 421 tggtcaggtt tgtgaatctt atctcagaga ggaagacaaa gtttgccaag gtccccctca 481 agtgtctggc tcaagaggta aatattccgg attggattgt tgaccttcgc catgagttga 541 cccacaagaa aatgccccat ataaatgact gccgcagagg ctgctacttt gtcctggatt 601 ggctccagaa gacctattgg tgccgccaac tggagaacag cctgagagag acctgggagt 661 tggaggagtt cagggaaggg atagaggaag aggatcaaga ggaagataag aacattgttg 721 ttgatgacat cacagaacag aaaccagagc ctcaggatga tgggaaaagt acggagtcag 781 atgtaaaggc cgatggagac agcaaaggca gcgaagaggt ggattctcat tgcaaaaagg 841 ccctgagtca taaagagcta tatgaaagag cccgagaact gctggtatca tacgaagagg 901 agcagtttac ggtgctggag aaatttaggt atttacctaa ggccattaag gcgtggaata 961 acccgtcccc acgtgtagaa tgtgtcctgg cagagctcaa gggcgttaca tgcgagaaca 1021 gggaggctgt gctggatgct tttctggatg atggcttcct tgtccccaca tttgaacagt 1081 tggcagcttt gcagatagaa tatgaagaaa acgtggactt gaatgacgtc ctggtgccaa 1141 agccgttctc tcagttctgg cagcccctgc tcaggggcct gcactcccag aacttcacgc 1201 aggccctatt ggagaggatg ctctctgaac tgccagcctt ggggatcagc gggatccggc 1261 ctacctacat cctcagatgg accgttgaac tgatcgtggc caacaccaag actggacgga 1321 atgctcgccg attttctgca ggccagtggg aagcaagaag gggctggagg ctgttcaact 1381 gctccgcctc ccttgactgg ccccggatgg ttgagtcctg cttgggctca ccttgctggg 1441 ccagccccca actccttcgg atcatcttca aagccatggg gcagggcctg ccagacgagg 1501 agcaggagaa gctgctgcgc atctgttcca tttataccca gagtggagaa aacagcctgg 1561 tgcaggaggg ctctgaggcc tcccccattg ggaagtctcc atatacacta gacagcctgt 1621 attggagcgt caagccagcc agctccagct tcgggtctga agcaaaggcc cagcaacagg 1681 aggagcaggg cagtgttaat gatgtcaagg aagaggagaa ggaggagaaa gaggtcttgc 1741 cagaccaggt agaggaggag gaagaaaatg atgaccaaga ggaggaagag gaggatgaag 1801 atgatgaaga tgatgaagag gaagacagaa tggaggtggg gcctttctct acagggcaag 1861 agtcccccac tgccgagaat gctaggcttc tggcccagaa aagaggagct ttgcagggct 1921 ctgcatggca ggttagctca gaagacgtgc gatgggacac atttccccta ggccgaatgc 1981 caggtcagac cgaggaccca gcagagctca tgctggagaa ttatgacacc atgtatcttt 2041 tggaccagcc tgtgctagag cagcggctgg aaccctcaac atgcaagact gacaccttgg 2101 gcctgagctg tggtgtcggc agtggcaact gcagcaacag cagcagcagc aacttcgagg 2161 gccttctctg gagccagggg cagctgcatg ggctcaaaac tggcctgcag ctcttctnnc 2221 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 2281 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 2341 tta