Construct: ORF ccsbBroad304_16019
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003061.1_s304c1, BRDN0000404480
- Derived from:
- ccsbBroadEn_16019
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TTC25 (83538)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_16019
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83538 | TTC25 | tetratricopeptide repeat do... | NM_001350319.2 | 60.7% | 47.2% | 127C>A;626_1058del;1310_1440del |
| 2 | human | 83538 | TTC25 | tetratricopeptide repeat do... | NM_031421.5 | 43.4% | 33.8% | 127C>A;626_1058del;1310_2016del |
| 3 | human | 83538 | TTC25 | tetratricopeptide repeat do... | NR_110662.3 | 32.2% | 1_107del;234C>A;984_2713del | |
| 4 | human | 83538 | TTC25 | tetratricopeptide repeat do... | NR_146621.2 | 28.5% | (many diffs) | |
| 5 | mouse | 74407 | Ttc25 | tetratricopeptide repeat do... | XM_011249287.2 | 49.5% | 37.1% | (many diffs) |
| 6 | mouse | 74407 | Ttc25 | tetratricopeptide repeat do... | XM_006534365.3 | 41% | 31% | (many diffs) |
| 7 | mouse | 74407 | Ttc25 | tetratricopeptide repeat do... | NM_028918.2 | 40.2% | 30.4% | (many diffs) |
| 8 | mouse | 74407 | Ttc25 | tetratricopeptide repeat do... | XR_388541.3 | 29.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 942
- ORF length:
- 876
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc ggaccccgaa ggcgagacct tgcgaagcac ctttccctct tatatggccg 121 aaggcgagcg gctctacctg tgcggggaat tttctaaagc cgcgcagagc ttcagcaacg 181 ctctttacct taaggatgga gacaagaact gcctggttgc tcgctcaaag tgcttcctga 241 agatgggaga cttggagaga tccctgaagg atgctgaggc ttcgctccag agtgacccag 301 ctttctgtaa ggggattttg caaaaggctg agacactgta caccatggga gactttgagt 361 ttgccttggt attctatcat cgaggctaca agctgaggcc tgatcgggaa ttcagagttg 421 gcattcagaa agcccaggaa gccatcaaca actcagtggg aagtccttct tccattaagc 481 tggagaacaa aggggacctc tccttcttaa gcaagcaggc tgagaatata aaagcccagc 541 agaagcctca gcccatgaaa cacctcttac accccaccaa gggagagccc aagtggaagg 601 cctcgctcaa gagtgagaag actgtccgcc agcttctggg ggagctctac gtggacaaag 661 agtatttgga gaagctccta ttggatgaag tgaccttcct gatgcaaaat cgagagccct 721 tgacaacatt ggcagagttt ttgccagagt tgggaaattc cagcaagcca ttgacacgtg 781 ggaagaaaag atccctctgg caaaaaccac cctggagaag acctggctgt tccacgagat 841 cggccgctgc tacttggagc tggaccaggc ctggcaggcc cagaattatg gcgagaagtc 901 ccagcagtgt gccgaggagg aaggggacat tgagtggcaa ctnnccaact ttcttgtaca 961 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 1021 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta