Construct: ORF ccsbBroad304_16053
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001543.1_s304c1, BRDN0000401523
- Derived from:
- ccsbBroadEn_16053
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAATS1 (89876)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_16053
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 89876 | MAATS1 | MYCBP associated and testis... | NM_033364.4 | 99.9% | 99.8% | 258T>C;619G>C |
| 2 | human | 89876 | MAATS1 | MYCBP associated and testis... | NM_001320316.1 | 96% | 92.4% | (many diffs) |
| 3 | human | 89876 | MAATS1 | MYCBP associated and testis... | NM_001320317.1 | 91.9% | 91.2% | 0_1ins109;14_15ins77 |
| 4 | human | 89876 | MAATS1 | MYCBP associated and testis... | NM_001320318.1 | 83.5% | 83.5% | 0_1ins378 |
| 5 | human | 89876 | MAATS1 | MYCBP associated and testis... | XM_011513277.1 | 83.5% | 83.4% | 0_1ins378;241G>C |
| 6 | human | 89876 | MAATS1 | MYCBP associated and testis... | XR_924211.1 | 51.4% | (many diffs) | |
| 7 | human | 89876 | MAATS1 | MYCBP associated and testis... | XR_924210.1 | 51% | (many diffs) | |
| 8 | human | 89876 | MAATS1 | MYCBP associated and testis... | XR_001740354.1 | 48.7% | (many diffs) | |
| 9 | human | 89876 | MAATS1 | MYCBP associated and testis... | XR_001740355.1 | 48.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2370
- ORF length:
- 2301
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gagccacgca gtaaccatcg aggagcccca ggcccagccg caggtgtctc 121 aaactcggta ccgggagagg tcgcgggctg ggagccacat ctcctccaat cgagcgtatg 181 attttctgta cgatccattg tttattgtgt caagtgagaa agaccataca caggcaaata 241 tccaagctac cctgattcgc agcagactga gaaaagttcc caggtttaaa accatgttca 301 gtaacctgat ccattatcca agatactctc tatattggag caagtcagat cctgtcccac 361 catttatcag tcgggaatgg aagggacata aggagaaaca cagagaagcc ctccggcagc 421 tcaccacaac tgacgcttct tttcagatgc ctaaagaagt ttatgaagat cctgaagtta 481 ctggaaagaa tcgctataaa tactttgaaa ggccttttct cccatttttt cagcagatgc 541 cattcaatgt tgtttatgcc gtatccaagg cagaaccata cacttttcct cctacttcta 601 ctaagcacct atccatccct tcaaagtcta ctgtgggcac tcagactgat tatcgggatg 661 ctgacgttca aacagatcca tactctccag aatatgtagt atgtcaggac tcaatccctg 721 agctcttgac cctggctacg cttacttggg gtcggggtct cccagcagga caagctgagg 781 tggagatgat agaaagagcc cgcgagaagc gtgcttggga agcctctctc cccgctctga 841 gtgacacctc ccagtttgag aagaggagga aaatgatgaa tgaaatggag aggaaggagt 901 gggccttcag agagcaggag attgaaaaac tgcaggagat tcgcctggaa gttctaaaag 961 agctgttgag gaagcgtgaa gagaatcaga atgaagtgaa tatgaagcac ttgaatgccc 1021 ggtggtctaa actgcaggag ggaaaagagg caaaaatggc aaaaattcag cgcacgcatg 1081 tatcaacaat cagaaaactt gtaggaaaga gaaagaatat agaagggaag ttggagagaa 1141 gaaatatcat caaggattat tctgattatg catcacaggt ctatggacct ctgtctcgtc 1201 ttgggtgttt cccagacaac aactcagagg actttgtagt aaaaaactac tatctcaaca 1261 cctatgaagg attagtggaa cttgagtcat gtctcccaga ttttgtgaca caaccccaaa 1321 tcagagctcc aaaacctaaa gtcattacca ccaaagctgg ttttctgaag agggcagcaa 1381 ggttggacta tgagttggca gaggttcata aggcactgtt ggataagaag aataaagttc 1441 ttgaagtaaa gaaaccccct cgcttccttc aaagaaaccc aatacctcaa cctcggcttc 1501 caactccaac cttggaaatg acgtccaatg aagaagaaga aatggaaatg gctgtgatct 1561 accttcaaaa gttactccgg ggcagagtcg ttcagaacat gatgtttgaa gggaaagaaa 1621 agcgactgga gttgatccag gagttgcgca cctgccacgc actacaagaa gatgaaaagc 1681 tggtgaaaaa agccgagaag caagtgaccc tggccttaca gcggcagagg aacttgcatg 1741 agcacaaggt gtcactggtt gaaaaccatt tggccggact ggaaggaagg gcactagcag 1801 acatgtttga cttcctgtcc aaagagctgg tgagactgca ggaggagagg aggatccatg 1861 cctttgtcat gctggctgag cgccagcggc gggtacgaga ggctgaagag agtggtcggc 1921 gccaggtgga aaaacagcgc ctgcgggagg aggacgagat atttaaggag gtggttaaag 1981 ttcaccatag tactataagc tcctacctag aagacataat actgaatacc gaagcgaata 2041 ctgcagaaga acaagccagg gcagaaatag agaagatggc tgagaaaatc aatgacattg 2101 cttatgaaat ggaaagccgc cgaacctatc ttcagtcaga ggagattgtt gctgagttgg 2161 tttatagttt tctgatccca gaggtgcaaa aatactttgt caaagaaaaa gtgaggaacg 2221 cacagcggaa acatattctt gcagcccatc agatcatcca cagttacacg gaaagcatgg 2281 ttcaaaagaa attaactgag ggagagcaag atgaggcctc aaatgctgcc atgttacttg 2341 agaaagaaac tcaaaatgag aacaacagct nnccaacttt cttgtacaaa gtggttggta 2401 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 2461 ggtggcgcgt taagtcgaca atcaacctct ggatta