Transcript: Human NM_001320317.1

Homo sapiens MYCBP associated and testis expressed 1 (MAATS1), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-09-25
Taxon:
Homo sapiens (human)
Gene:
MAATS1 (89876)
Length:
4372
CDS:
187..2304

Additional Resources:

NCBI RefSeq record:
NM_001320317.1
NBCI Gene record:
MAATS1 (89876)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001320317.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000134935 CCTGATCCATTATCCAAGATA pLKO.1 237 CDS 100% 5.625 7.875 N MAATS1 n/a
2 TRCN0000135140 CAAGGCAGAACCATACACTTT pLKO.1 498 CDS 100% 4.950 6.930 N MAATS1 n/a
3 TRCN0000134634 GTTACTGGAAAGAATCGCTAT pLKO.1 409 CDS 100% 4.050 5.670 N MAATS1 n/a
4 TRCN0000134053 CCAGTAAGGAAGCAACTAAAT pLKO.1 3688 3UTR 100% 13.200 9.240 N MAATS1 n/a
5 TRCN0000135381 GCACAGCGGAAACATATTCTT pLKO.1 2152 CDS 100% 5.625 3.938 N MAATS1 n/a
6 TRCN0000138445 CCAAGGCAGAACCATACACTT pLKO.1 497 CDS 100% 4.950 3.465 N MAATS1 n/a
7 TRCN0000134108 CCATCATAACTGCATACTCAA pLKO.1 3922 3UTR 100% 4.950 3.465 N MAATS1 n/a
8 TRCN0000134107 CCTGATTCAAAGACTGTCAAA pLKO.1 3002 3UTR 100% 4.950 3.465 N MAATS1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001320317.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_16053 pDONR223 0% 91.9% 91.2% None 0_1ins109;14_15ins77 n/a
2 ccsbBroad304_16053 pLX_304 0% 91.9% 91.2% V5 0_1ins109;14_15ins77 n/a
3 TRCN0000466472 ATTCCGTGTCGAAGTCTTCGCCTG pLX_317 18.8% 91.9% 91.2% V5 0_1ins109;14_15ins77 n/a
4 ccsbBroadEn_09280 pDONR223 100% 91.8% 91.1% None (many diffs) n/a
5 ccsbBroad304_09280 pLX_304 0% 91.8% 91.1% V5 (many diffs) n/a
6 TRCN0000474624 CAACTCCTCAGCCCCTTTCTGTTA pLX_317 16% 91.8% 91.1% V5 (many diffs) n/a
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