Construct: ORF ccsbBroad304_16090
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003057.1_s304c1, BRDN0000405898
- Derived from:
- ccsbBroadEn_16090
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AP1S3 (130340)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_16090
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 130340 | AP1S3 | adaptor related protein com... | XM_011510600.3 | 91.2% | 89.1% | (many diffs) |
| 2 | human | 130340 | AP1S3 | adaptor related protein com... | NM_001039569.2 | 65.8% | 64.2% | (many diffs) |
| 3 | human | 130340 | AP1S3 | adaptor related protein com... | NR_110905.2 | 7.7% | 1_132del;445_4025del | |
| 4 | human | 130340 | AP1S3 | adaptor related protein com... | NR_110906.2 | 7.2% | (many diffs) | |
| 5 | mouse | 252903 | Ap1s3 | adaptor-related protein com... | NM_183027.2 | 58.9% | 60.3% | (many diffs) |
| 6 | mouse | 252903 | Ap1s3 | adaptor-related protein com... | XR_373542.1 | 18.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 378
- ORF length:
- 312
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgat acatttcata ttgctcttca gtcgacaagg gaaattacgg ctacagaaat 121 ggtacatcac tctccctgat aaagagagga agaagatcac ccgggaaatt gttcagatta 181 ttctctcccg tggtcacagg acaagcagtt ttgttgactg gaaggagcta aaacttgttt 241 ataaaaggta tgctagttta tatttttgct gtgcaataga aaatcaggac aatgagctct 301 tgacgctaga gattgtgcat cgttacgtgg agctgctgga caaatatttt ggaaatactt 361 ggccttttgc aagagcctnn ccaactttct tgtacaaagt ggttggtaag cctatcccta 421 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 481 agtcgacaat caacctctgg atta