Transcript: Human NM_001039569.2

Homo sapiens adaptor related protein complex 1 subunit sigma 3 (AP1S3), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-22
Taxon:
Homo sapiens (human)
Gene:
AP1S3 (130340)
Length:
3986
CDS:
133..597

Additional Resources:

NCBI RefSeq record:
NM_001039569.2
NBCI Gene record:
AP1S3 (130340)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001039569.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000059614 CGCTAGAGATTGTGCATCGTT pLKO.1 371 CDS 100% 3.000 4.200 N AP1S3 n/a
2 TRCN0000059617 GACAATGAGCTCTTGACGCTA pLKO.1 355 CDS 100% 2.640 3.696 N AP1S3 n/a
3 TRCN0000059615 CGACAAGGGAAATTACGGCTA pLKO.1 160 CDS 100% 2.160 3.024 N AP1S3 n/a
4 TRCN0000059613 CGGGAAATTGTTCAGATTATT pLKO.1 229 CDS 100% 15.000 12.000 N AP1S3 n/a
5 TRCN0000059616 GTACATCACTCTCCCTGATAA pLKO.1 189 CDS 100% 13.200 9.240 N AP1S3 n/a
6 TRCN0000113119 GTGAGCTGGATATTATCTTTA pLKO.1 428 CDS 100% 13.200 9.240 N Ap1s3 n/a
7 TRCN0000007228 CACCTGTAATCCCAGCACTTT pLKO.1 1744 3UTR 100% 4.950 2.475 Y CFLAR n/a
8 TRCN0000166635 CACCTGTAATCCCAGCACTTT pLKO.1 1744 3UTR 100% 4.950 2.475 Y C19orf31 n/a
9 TRCN0000166201 CATGGTGAAACCCTGTCTCTA pLKO.1 3579 3UTR 100% 4.950 2.475 Y ORAI2 n/a
10 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 3502 3UTR 100% 4.950 2.475 Y ERAP2 n/a
11 TRCN0000140719 GATCACTTGAGGTCAGGAGTT pLKO.1 1783 3UTR 100% 4.050 2.025 Y P3H4 n/a
12 TRCN0000165299 GATCACTTGAGGTCAGGAGTT pLKO.1 1783 3UTR 100% 4.050 2.025 Y ORAI2 n/a
13 TRCN0000352971 GATCACTTGAGGTCAGGAGTT pLKO_005 1783 3UTR 100% 4.050 2.025 Y P3H4 n/a
14 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 3503 3UTR 100% 13.200 6.600 Y LIAS n/a
15 TRCN0000179120 CAACATGGTGAAACCCTGTTT pLKO.1 3576 3UTR 100% 4.950 2.475 Y LOC339059 n/a
16 TRCN0000138772 GCAGGAGAATCGCTTGAACTT pLKO.1 2045 3UTR 100% 4.950 2.475 Y DCAF11 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001039569.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13154 pDONR223 100% 90.9% 87.1% None (many diffs) n/a
2 ccsbBroad304_13154 pLX_304 0% 90.9% 87.1% V5 (many diffs) n/a
3 TRCN0000471180 ACCCTAGACTGGACCGGGACCCCC pLX_317 80.4% 90.9% 87.1% V5 (many diffs) n/a
4 ccsbBroadEn_16090 pDONR223 0% 65.8% 64.2% None (many diffs) n/a
5 ccsbBroad304_16090 pLX_304 0% 65.8% 64.2% V5 (many diffs) n/a
6 TRCN0000474332 AACAACTTCACCACCTTTAGTTCA pLX_317 100% 63.4% 63.6% V5 (not translated due to frame shift) (many diffs) n/a
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