Construct: ORF ccsbBroad304_16122

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013594.1_s304c1, BRDN0000400358
Derived from:
ccsbBroadEn_16122
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ADAM32 (203102)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_16122
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 203102 ADAM32 ADAM metallopeptidase domai... NM_145004.7 99.8% 99.6% 293A>G;478A>G;1400C>G
2 human 203102 ADAM32 ADAM metallopeptidase domai... XM_006716298.3 98.2% 97.9% (many diffs)
3 human 203102 ADAM32 ADAM metallopeptidase domai... XM_011544433.2 98.2% 97.9% (many diffs)
4 human 203102 ADAM32 ADAM metallopeptidase domai... XM_017013183.2 96.9% 96.6% (many diffs)
5 human 203102 ADAM32 ADAM metallopeptidase domai... XM_017013184.2 94.9% 94.6% (many diffs)
6 human 203102 ADAM32 ADAM metallopeptidase domai... XM_017013185.2 94% 93.7% (many diffs)
7 human 203102 ADAM32 ADAM metallopeptidase domai... XM_024447092.1 93.2% 92.6% (many diffs)
8 human 203102 ADAM32 ADAM metallopeptidase domai... XM_011544434.2 90.5% 90.2% (many diffs)
9 human 203102 ADAM32 ADAM metallopeptidase domai... XM_006716299.2 86.4% 86.1% (many diffs)
10 human 203102 ADAM32 ADAM metallopeptidase domai... XM_017013186.2 83.3% 83.1% (many diffs)
11 human 203102 ADAM32 ADAM metallopeptidase domai... XM_024447093.1 79.7% 79.1% (many diffs)
12 human 203102 ADAM32 ADAM metallopeptidase domai... NM_001313994.1 79.6% 78.9% (many diffs)
13 human 203102 ADAM32 ADAM metallopeptidase domai... XM_017013187.1 64.6% 64.5% 0_1ins831;3_5delGAG;572C>G
14 human 203102 ADAM32 ADAM metallopeptidase domai... XM_017013188.1 59.1% 59% 0_1ins963;437C>G
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2427
ORF length:
2361
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtt ccgcctctgg ttgctgctgg ccgggctctg cggcctcctg gcgtcaagac
121ccggttttca aaattcactt ctacagatcg taattccaga gaaaatccaa acaaatacaa
181atgacagttc agaaatagaa tatgaacaaa tatcctatat tattccaata gatgagaaac
241tgtacactgt gcaccttaaa caaagatatt ttttagcaga taattttatg atctatttgt
301acaatcaagg atctatgaat acttattctt cagatattca gactcaatgc tactatcgag
361gaaatattga aggatatcca gattccatgg tcacactcag cacgtgctct ggactaagag
421gaatactgca atttgaaaat gtttcttatg gaattgagcc tctggaatct gcagttgaat
481ttcagcatgt tctttacaaa ttaaagaatg aagacaatga tattgcaatt tttattgaca
541gaggcctgaa agaacaacca atggatgaca acatttttat aagtgaaaaa tcagaaccag
601ctgttccaga tttatttcct ctttatctag aaatgcatat tgtggtggac aaaactttgt
661atgattactg gggctctgat agcatgatag taacaaataa agtcatcgaa attgttggcc
721ttgcaaattc aatgttcacc caatttaaag ttactattgt gctgtcatca ttggagttat
781ggtcagatga aaataagatt tctacagttg gtgaggcaga tgaattattg caaaaatttt
841tagaatggaa acaatcttat cttaacctaa ggcctcatga tattgcatat ctactaattt
901atatggatta tcctcgttat ttgggagcag tgtttcctgg aacaatgtgt attactcgtt
961attctgcagg agttgcattg taccccaagg agataactct ggaggcattt gcagttattg
1021tcacccagat gctggcactc agtctgggaa tatcatatga cgacccaaag aaatgtcaat
1081gttcagaatc cacctgtata atgaatccag aagttgtgca atccaatggt gtgaagactt
1141ttagcagttg cagtttgagg agctttcaaa atttcatttc aaatgtgggt gtcaaatgtc
1201ttcagaataa gccacaaatg caaaaaaaat ctccgaaacc agtctgtggc aatggcagat
1261tggagggaaa tgaaatctgt gattgtggta ctgaggctca atgtggacct gcaagctgtt
1321gtgattttcg aacttgtgta ctgaaagacg gagcaaaatg ttataaagga ctgtgctgca
1381aagactgtca aattttacaa tcaggcgttg aatgtaggcc gaaagcacat cctgaatgtg
1441acatcgctga aaattgtaat ggaagctcac cagaatgtgg tcctgacata actttaatca
1501atggactttc atgcaaaaat aataagttta tttgttatga cggagactgc catgatctcg
1561atgcacgttg tgagagtgta tttggaaaag gttcaagaaa tgctccattt gcctgctatg
1621aagaaataca atctcaatca gacagatttg ggaactgtgg tagggataga aataacaaat
1681atgtgttctg tggatggagg aatcttatat gtggaagatt agtttgtacc taccctactc
1741gaaagccttt ccatcaagaa aatggtgatg tgatttatgc tttcgtacga gattctgtat
1801gcataactgt agactacaaa ttgcctcgaa cagttccaga tccactggct gtcaaaaatg
1861gctctcagtg tgatattggg agggtttgtg taaatcgtga atgtgtagaa tcaaggataa
1921ttaaggcttc agcacatgtt tgttcacaac agtgttctgg acatggagtg tgtgattcca
1981gaaacaagtg ccattgttcg ccaggctata agcctccaaa ctgccaaata cgttccaaag
2041gattttccat atttcctgag gaagatatgg gttcaatcat ggaaagagca tctgggaaga
2101ctgaaaacac ctggcttcta ggtttcctca ttgctcttcc tattctcatt gtaacaaccg
2161caatagtttt ggcaaggaaa cagttgaaaa agtggttcgc caaggaagag gaattcccaa
2221gtagcgaatc taaatcggaa ggtagcacac agacatatgc cagccaatcc agctcagaag
2281gcagcactca gacatatgcc agccaaacca gatcagaaag cagcagtcaa gctgatacta
2341gcaaatccaa atcagaagat agtgctgaag catatactag cagatccaaa tcacaggaca
2401gtacccaaac acaaagcagt agtaactnnc caactttctt gtacaaagtg gttggtaagc
2461ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt
2521ggcgcgttaa gtcgacaatc aacctctgga tta

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