Construct: ORF ccsbBroad304_16126
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004943.1_s304c1, BRDN0000399858
- Derived from:
- ccsbBroadEn_16126
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM124A (220108)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_16126
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 220108 | FAM124A | family with sequence simila... | NM_001330522.1 | 95.2% | 94.6% | 852_853insTC;863_903del |
| 2 | human | 220108 | FAM124A | family with sequence simila... | XM_017020419.2 | 92.9% | 92.1% | (many diffs) |
| 3 | human | 220108 | FAM124A | family with sequence simila... | XM_011534978.2 | 83.1% | 82.1% | (many diffs) |
| 4 | human | 220108 | FAM124A | family with sequence simila... | NM_001242312.2 | 52.2% | 50.5% | (many diffs) |
| 5 | human | 220108 | FAM124A | family with sequence simila... | NM_145019.4 | 49% | 47.2% | (many diffs) |
| 6 | mouse | 629059 | Fam124a | family with sequence simila... | NM_001243857.1 | 45.8% | 47.4% | (many diffs) |
| 7 | mouse | 629059 | Fam124a | family with sequence simila... | XM_006519347.3 | 40.7% | 42% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 930
- ORF length:
- 864
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga cccaaaggcg ggcggcggcg gcgaggagga cgactgcgtg gactcgggcg 121 ccgagaccgg agggtccgac tacagccacc tgtcctccac gagcagtgag ctttccgttg 181 aagaggcgca ggaccctttc ctggtcagca tccacataat cgcagaccca ggggagtccc 241 agcccctgca ggaggccatc gacaacgtcc tggcgtggat ccaccccgac ctcccgctgt 301 tccgggtgtc cgagaggcgg gcgtcccggc ggcggcggaa gccccccaag ggcgctcagc 361 cagcgctggc tgtggtgctg ttcctgcagg aggagtacgg cgaagagcag atcctgcagc 421 tgcaccgcac actgcagcag ccgccctggc gccaccacca caccgagcag gtgcacggcc 481 ggttcctgcc ctacctgccc tgcagccagg acttcttcac gctggcccct gggacgccgc 541 tttgggccat ccggcccgtg cactacggca aggaaatcgt gcgcttcacc gtctactgtc 601 gctacgacaa ctatgctgac agcctcaggt tctaccagct gattctccgg aggagcccca 661 gccagaagaa agcggacttc tgcatcttcc ctattttttc caacctggat gtggacatcc 721 agttctccct gaaaagactg ccctgtgacc agtgcccggt gcccaccgac tcctccgtgc 781 tggagttccg agtgagggac ataggcgagc tcgtgcctct cctgcccaac ccttgcagcc 841 ccatcagcga ggggcgctgg cagacggagg accatgatgg gaacaagatc ctcctacagg 901 tactgggggg acgcctgtct ctgtctgttt nnccaacttt cttgtacaaa gtggttggta 961 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 1021 ggtggcgcgt taagtcgaca atcaacctct ggatta