Construct: ORF ccsbBroad304_16128
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008943.1_s304c1, BRDN0000412037
- Derived from:
- ccsbBroadEn_16128
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC39A12 (221074)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_16128
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 221074 | SLC39A12 | solute carrier family 39 me... | NM_152725.3 | 61.4% | 54.1% | (many diffs) |
| 2 | human | 221074 | SLC39A12 | solute carrier family 39 me... | NM_001282733.1 | 58.2% | 51.3% | (many diffs) |
| 3 | human | 221074 | SLC39A12 | solute carrier family 39 me... | NM_001145195.2 | 58.1% | 51.2% | (many diffs) |
| 4 | human | 221074 | SLC39A12 | solute carrier family 39 me... | NM_001282734.1 | 38.8% | 32.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1281
- ORF length:
- 1212
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtgcttccgg acaaagctct cagtatcctg ggtgccattg tttcttctac 121 tcagccgtgt tttttctact gagacagaca aaccctcagc ccaggacagc agaagccgtg 181 ggagttcagg ccaaccggca gacctgctac aggttctctc tgctggtgac cacccacccc 241 acaaccactc aagaagcctc atcaaaacat tgttggagaa aactgggtgc ccacggagga 301 gaaacggaat gcaaggagat tgcaatctgt gctttgaacc agatgcacta ttactaatag 361 ctggaggaaa ttttgaagat cagcttagag aagaagtggt ccagagagtt tctcttctcc 421 ttctctatta cattattcat caggaagaga tctgttcttc aaagctcaac atgagtaata 481 aagagtataa attttaccta cacagcctac tgagcctcag gcaggatgaa gattcctctt 541 tcctttcaca gaatgagaca gaagatatct tggctttcac caggcagtac tttgacactt 601 ctcaaagcca gtgtatggaa accaaaacgc tgcagaaaaa atctggaata gtgagcagtg 661 aaggtgctaa tgaaagtacg cttcctcagt tggcagccat gatcattact ttgtccctcc 721 agggtgtttg tctgggacaa ggaaacttgc cttcaccaga ctactttaca gaatatattt 781 tcagttcctt gaatcgtacg aatacactcc gcctatcaga actagaccaa ctcctcaaca 841 ctctctggac cagaagtact tgtatcaaaa atgagaaaat ccatcaattt caaaggaaac 901 aaaacaacat aataacccat gatcaggact attctaattt ctcttcatcc atggaaaaag 961 agtctgagga tggtccaatt tcctgggatc agacctgctt ctctgctagg cagcgtgtgg 1021 agatatttct acagaagggc ctctcactca tttctaagga ggactttaag caaatgagtc 1081 cagggatcat ccagcgctgg tccttttcca tagctgtgag gagaactaca ggcttatctt 1141 acagctgttt gtgggcttgg ccgtcgggac actgtctggg gacgctctgc tccaccttat 1201 ccctcaggtt cttggtttac ataagcagga agccccagaa tttgggcatt tccatgaaag 1261 caaaggtcat atttggaaac tnnccaactt tcttgtacaa agtggttggt aagcctatcc 1321 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 1381 ttaagtcgac aatcaacctc tggatta