Construct: ORF ccsbBroad304_16141
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000735.1_s304c1, BRDN0000404953
- Derived from:
- ccsbBroadEn_16141
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NSUN5P2 (260294)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_16141
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | NM_001168348.2 | 28.5% | 27.8% | (many diffs) |
| 2 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | NM_018044.4 | 26% | 25.4% | (many diffs) |
| 3 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | NM_148956.4 | 23.9% | 23.3% | (many diffs) |
| 4 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | NM_001168347.3 | 23.7% | 23.1% | (many diffs) |
| 5 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | XR_242255.5 | 20.5% | (many diffs) | |
| 6 | human | 260294 | NSUN5P2 | NSUN5 pseudogene 2 | NR_033323.3 | 19.6% | 1_1112del;1463_1784delinsG | |
| 7 | human | 155400 | NSUN5P1 | NSUN5 pseudogene 1 | NR_033322.3 | 19.4% | (many diffs) | |
| 8 | human | 155400 | NSUN5P1 | NSUN5 pseudogene 1 | NR_104013.1 | 18.4% | (many diffs) | |
| 9 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | XM_005277604.5 | 15.9% | 6.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 417
- ORF length:
- 351
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc gagcagacag ctggaggagc ccggggcagg gacacctagc ccggtgcgtc 121 tgcatgccct ggcagggttc cagcagcgag ccctgtgcca cgcgctcact ttcccttccc 181 tgcagcggct cgtctactcc atgtgctccc tctgccagga ggagaatgaa gacatggtac 241 aagatgcgct gcagcagaac ccgggcgcct tcaggctagc tcccgccctg cctgcccggc 301 cccaccgagg cctgagcacg ttcccgggtg ccgagcactg cctccgggct tcccccaaga 361 ccacgcttag cggtggcttc ttcgttgctg taattgaacg ggtcgagatg ccgacgtnnc 421 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 481 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 541 tta