Construct: ORF ccsbBroad304_16151
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015124.1_s304c1, BRDN0000405340
- Derived from:
- ccsbBroadEn_16151
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DOK7 (285489)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_16151
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285489 | DOK7 | docking protein 7 | NM_173660.5 | 99.9% | 99.8% | 1382G>A |
| 2 | human | 285489 | DOK7 | docking protein 7 | NM_001301071.2 | 82.6% | 82.2% | (many diffs) |
| 3 | human | 285489 | DOK7 | docking protein 7 | XM_011513435.2 | 79% | 78.6% | (many diffs) |
| 4 | human | 285489 | DOK7 | docking protein 7 | NM_001363811.2 | 58.9% | 58.3% | (many diffs) |
| 5 | human | 285489 | DOK7 | docking protein 7 | XM_011513437.2 | 57.3% | 50.5% | (many diffs) |
| 6 | human | 285489 | DOK7 | docking protein 7 | NM_001164673.2 | 50.5% | 37% | 521_522insGTGTGGGTACT;765_766ins736 |
| 7 | human | 285489 | DOK7 | docking protein 7 | NM_001256896.1 | 38.4% | 38.2% | 0_1ins930;452G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1581
- ORF length:
- 1512
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gaccgaggcg gcgctggtgg agggccaggt caagctgcgg gacggcaaga 121 agtggaagag taggtggctg gtgctgcgga agccgtcgcc cgtggcagac tgcctgctga 181 tgctggtcta caaggacaag tcggagcgta tcaagggcct gcgggagcgc agcagcctga 241 cgctagagga catctgcggg ctggagcccg gcctgcccta cgagggcctg gtccacacgc 301 tggccattgt ctgcctgtcc caggccatca tgctgggctt tgacagccac gaggccatgt 361 gtgcgtggga tgcccggatc cgctatgcgc tcggcgaggt gcataggttc catgtgacag 421 tggctccagg caccaagttg gagagcggcc cggctaccct gcacctctgc aatgatgtcc 481 tcgtcttggc cagggacatc cccccggctg tcacggggca gtggaagctg tctgacctcc 541 ggcgctacgg ggccgtgcca agcggattca tctttgaagg cgggaccagg tgtgggtact 601 gggctggcgt cttcttcctg tcctcggccg agggggagca gatcagcttc ctgttcgact 661 gcatcgtccg aggcatctcc cccaccaagg gcccctttgg gctgcggccg gttctaccag 721 acccaagtcc cccgggaccc tcgactgtgg aggagcgtgt ggcccaggaa gccctggaaa 781 ccctacagct ggagaagcgg ctgagcctcc tctcacatgc gggcaggccg ggcagtggag 841 gggatgaccg cagcctgtcc agctcatcct cagaggccag tcacttggac gtcagcgcca 901 gcagccggct caccgcatgg ccagagcaat cctcgtcgtc agccagcacg tcacaggagg 961 ggcctagacc agcagctgcc caggccgccg gggaagccat ggtgggtgcc tcaaggccac 1021 cccccaagcc gctgcgtccg cggcagctgc aggaggttgg ccgccagagc tcctcggaca 1081 gcggcatcgc cactggcagc cactcctctt actccagcag cctctcgtcc tacgcgggca 1141 gcagcctgga cgtgtggcgg gccacagatg aactgggctc actgctcagc ctgccagcag 1201 cgggggcccc cgagcccagc ctgtgcacct gcctgcccgg gacagtcgag taccaggtgc 1261 ccacctccct gcgggcccac tatgacacac cacgcagcct ttgcctggct cctagagacc 1321 acagcccccc ctcacagggc agccccggca acagtgcggc cagggactca ggcggccaga 1381 cgtccgccgg gtgtccctct ggctggctgg gcacgagacg gcggggcctg gtgatggagg 1441 ccccccagga cagcgaggcc acactgcctg gccctgcccc tggcgagccc tgggaagcag 1501 gcggccccca cgcggggcca cccccggctt tcttttcggc atgtccagtc tgtggaggac 1561 tcaaggtaaa cccccctcct tnnccaactt tcttgtacaa agtggttggt aagcctatcc 1621 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 1681 ttaagtcgac aatcaacctc tggatta